The use of fluorescence enhancement to improve the microscopic diagnosis of falciparum malaria
Abstract Background Giemsa staining of thick blood smears remains the "gold standard" for detecting malaria. However, this method is not very good for diagnosing low-level infections. A method for the simultaneous staining of Plasmodium -parasitized culture and blood smears for both bright...
Published in: | Malaria Journal |
---|---|
Main Authors: | , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
BMC
2007
|
Subjects: | |
Online Access: | https://doi.org/10.1186/1475-2875-6-89 https://doaj.org/article/43802c63e50d4fa09b49f909453d2095 |
id |
ftdoajarticles:oai:doaj.org/article:43802c63e50d4fa09b49f909453d2095 |
---|---|
record_format |
openpolar |
spelling |
ftdoajarticles:oai:doaj.org/article:43802c63e50d4fa09b49f909453d2095 2023-05-15T15:09:22+02:00 The use of fluorescence enhancement to improve the microscopic diagnosis of falciparum malaria Liu Paul Guy Rebecca Pennefather Peter Crandall Ian 2007-07-01T00:00:00Z https://doi.org/10.1186/1475-2875-6-89 https://doaj.org/article/43802c63e50d4fa09b49f909453d2095 EN eng BMC http://www.malariajournal.com/content/6/1/89 https://doaj.org/toc/1475-2875 doi:10.1186/1475-2875-6-89 1475-2875 https://doaj.org/article/43802c63e50d4fa09b49f909453d2095 Malaria Journal, Vol 6, Iss 1, p 89 (2007) Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 2007 ftdoajarticles https://doi.org/10.1186/1475-2875-6-89 2022-12-31T08:47:50Z Abstract Background Giemsa staining of thick blood smears remains the "gold standard" for detecting malaria. However, this method is not very good for diagnosing low-level infections. A method for the simultaneous staining of Plasmodium -parasitized culture and blood smears for both bright field and fluorescence was developed and its ability to improve detection efficiency tested. Methods A total of 22 nucleic acid-specific fluorescent dyes were tested for their ability to provide easily observable staining of Plasmodium falciparum -parasitized red blood cells following Giemsa staining. Results Of the 14 dyes that demonstrated intense fluorescence staining, only SYBR Green 1, YOYO-1 and ethidum homodimer-2 could be detected using fluorescent microscopy, when cells were first stained with Giemsa. Giemsa staining was not effective when applied after the fluorescent dyes. SYBR Green 1 provided the best staining in the presence of Giemsa, as a very high percentage of the parasitized cells were simultaneously stained. When blood films were screened using fluorescence microscopy the parasites were more readily detectable due to the sharp contrast between the dark background and the specific, bright fluorescence produced by the parasites. Conclusion The dual staining method reported here allows fluorescence staining, which enhances the reader's ability to detect parasites under low parasitaemia conditions, coupled with the ability to examine the same cell under bright field conditions to detect the characteristic morphology of Plasmodium species that is observed with Giemsa staining. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Yoyo ENVELOPE(-121.470,-121.470,58.917,58.917) Malaria Journal 6 1 89 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
spellingShingle |
Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 Liu Paul Guy Rebecca Pennefather Peter Crandall Ian The use of fluorescence enhancement to improve the microscopic diagnosis of falciparum malaria |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
Abstract Background Giemsa staining of thick blood smears remains the "gold standard" for detecting malaria. However, this method is not very good for diagnosing low-level infections. A method for the simultaneous staining of Plasmodium -parasitized culture and blood smears for both bright field and fluorescence was developed and its ability to improve detection efficiency tested. Methods A total of 22 nucleic acid-specific fluorescent dyes were tested for their ability to provide easily observable staining of Plasmodium falciparum -parasitized red blood cells following Giemsa staining. Results Of the 14 dyes that demonstrated intense fluorescence staining, only SYBR Green 1, YOYO-1 and ethidum homodimer-2 could be detected using fluorescent microscopy, when cells were first stained with Giemsa. Giemsa staining was not effective when applied after the fluorescent dyes. SYBR Green 1 provided the best staining in the presence of Giemsa, as a very high percentage of the parasitized cells were simultaneously stained. When blood films were screened using fluorescence microscopy the parasites were more readily detectable due to the sharp contrast between the dark background and the specific, bright fluorescence produced by the parasites. Conclusion The dual staining method reported here allows fluorescence staining, which enhances the reader's ability to detect parasites under low parasitaemia conditions, coupled with the ability to examine the same cell under bright field conditions to detect the characteristic morphology of Plasmodium species that is observed with Giemsa staining. |
format |
Article in Journal/Newspaper |
author |
Liu Paul Guy Rebecca Pennefather Peter Crandall Ian |
author_facet |
Liu Paul Guy Rebecca Pennefather Peter Crandall Ian |
author_sort |
Liu Paul |
title |
The use of fluorescence enhancement to improve the microscopic diagnosis of falciparum malaria |
title_short |
The use of fluorescence enhancement to improve the microscopic diagnosis of falciparum malaria |
title_full |
The use of fluorescence enhancement to improve the microscopic diagnosis of falciparum malaria |
title_fullStr |
The use of fluorescence enhancement to improve the microscopic diagnosis of falciparum malaria |
title_full_unstemmed |
The use of fluorescence enhancement to improve the microscopic diagnosis of falciparum malaria |
title_sort |
use of fluorescence enhancement to improve the microscopic diagnosis of falciparum malaria |
publisher |
BMC |
publishDate |
2007 |
url |
https://doi.org/10.1186/1475-2875-6-89 https://doaj.org/article/43802c63e50d4fa09b49f909453d2095 |
long_lat |
ENVELOPE(-121.470,-121.470,58.917,58.917) |
geographic |
Arctic Yoyo |
geographic_facet |
Arctic Yoyo |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Malaria Journal, Vol 6, Iss 1, p 89 (2007) |
op_relation |
http://www.malariajournal.com/content/6/1/89 https://doaj.org/toc/1475-2875 doi:10.1186/1475-2875-6-89 1475-2875 https://doaj.org/article/43802c63e50d4fa09b49f909453d2095 |
op_doi |
https://doi.org/10.1186/1475-2875-6-89 |
container_title |
Malaria Journal |
container_volume |
6 |
container_issue |
1 |
container_start_page |
89 |
_version_ |
1766340581392384000 |