Neutralization of pharmacological and toxic activities of Bothrops jararacussu snake venom and isolated myotoxins by Serjania erecta methanolic extract and its fractions
Most of the snakebites recorded in Brazil are caused by the Bothrops genus. Given that the local tissue damage caused by this genus cannot be treated by antivenom therapy, numerous studies are focusing on supplementary alternatives, such as the use of medicinal plants. Serjania erecta has already de...
Published in: | Journal of Venomous Animals and Toxins including Tropical Diseases |
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ftdoajarticles:oai:doaj.org/article:437f453f16694a48bcbfacbe9c4f56ac 2023-05-15T15:09:28+02:00 Neutralization of pharmacological and toxic activities of Bothrops jararacussu snake venom and isolated myotoxins by Serjania erecta methanolic extract and its fractions RS Fernandes TR Costa S Marcussi CP Bernardes DL Menaldo II Rodriguéz Gonzaléz PS Pereira AM Soares 2011-01-01T00:00:00Z https://doi.org/10.1590/S1678-91992011000100011 https://doaj.org/article/437f453f16694a48bcbfacbe9c4f56ac EN eng SciELO http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992011000100011 https://doaj.org/toc/1678-9199 doi:10.1590/S1678-91992011000100011 1678-9199 https://doaj.org/article/437f453f16694a48bcbfacbe9c4f56ac Journal of Venomous Animals and Toxins including Tropical Diseases, Vol 17, Iss 1, Pp 85-93 (2011) medicinal plants antiophidian properties Serjania erecta Bothrops jararacussu snake venom myotoxins Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 article 2011 ftdoajarticles https://doi.org/10.1590/S1678-91992011000100011 2022-12-31T15:58:31Z Most of the snakebites recorded in Brazil are caused by the Bothrops genus. Given that the local tissue damage caused by this genus cannot be treated by antivenom therapy, numerous studies are focusing on supplementary alternatives, such as the use of medicinal plants. Serjania erecta has already demonstrated anti-inflammatory, antiseptic and healing properties. In the current study, the aerial parts of S. erecta were extracted with methanol, then submitted to chromatographic fractionation on a Sephadex LH20 column and eluted with methanol, which resulted in four main fractions. The crude extract and fractions neutralized the toxic activities of Bothrops jararacussu snake venom and isolated myotoxins (BthTX-I and II). Results showed that phospholipase A2, fibrinogenolytic, myotoxic and hemorrhagic activities were inhibited by the extract. Moreover, the myotoxic and edematous activities induced by BthTX-I, and phospholipase A2 activity induced by BthTX-II, were inhibited by the extract of S. erecta and its fraction. The clotting time on bovine plasma was significantly prolonged by the inhibitory action of fractions SF3 and SF4. This extract is a promising source of natural inhibitors, such as flavonoids and tannins, which act by forming complexes with metal ions and proteins, inhibiting the action of serineproteases, metalloproteases and phospholipases A2. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Journal of Venomous Animals and Toxins including Tropical Diseases 17 1 85 93 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
medicinal plants antiophidian properties Serjania erecta Bothrops jararacussu snake venom myotoxins Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 |
spellingShingle |
medicinal plants antiophidian properties Serjania erecta Bothrops jararacussu snake venom myotoxins Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 RS Fernandes TR Costa S Marcussi CP Bernardes DL Menaldo II Rodriguéz Gonzaléz PS Pereira AM Soares Neutralization of pharmacological and toxic activities of Bothrops jararacussu snake venom and isolated myotoxins by Serjania erecta methanolic extract and its fractions |
topic_facet |
medicinal plants antiophidian properties Serjania erecta Bothrops jararacussu snake venom myotoxins Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 |
description |
Most of the snakebites recorded in Brazil are caused by the Bothrops genus. Given that the local tissue damage caused by this genus cannot be treated by antivenom therapy, numerous studies are focusing on supplementary alternatives, such as the use of medicinal plants. Serjania erecta has already demonstrated anti-inflammatory, antiseptic and healing properties. In the current study, the aerial parts of S. erecta were extracted with methanol, then submitted to chromatographic fractionation on a Sephadex LH20 column and eluted with methanol, which resulted in four main fractions. The crude extract and fractions neutralized the toxic activities of Bothrops jararacussu snake venom and isolated myotoxins (BthTX-I and II). Results showed that phospholipase A2, fibrinogenolytic, myotoxic and hemorrhagic activities were inhibited by the extract. Moreover, the myotoxic and edematous activities induced by BthTX-I, and phospholipase A2 activity induced by BthTX-II, were inhibited by the extract of S. erecta and its fraction. The clotting time on bovine plasma was significantly prolonged by the inhibitory action of fractions SF3 and SF4. This extract is a promising source of natural inhibitors, such as flavonoids and tannins, which act by forming complexes with metal ions and proteins, inhibiting the action of serineproteases, metalloproteases and phospholipases A2. |
format |
Article in Journal/Newspaper |
author |
RS Fernandes TR Costa S Marcussi CP Bernardes DL Menaldo II Rodriguéz Gonzaléz PS Pereira AM Soares |
author_facet |
RS Fernandes TR Costa S Marcussi CP Bernardes DL Menaldo II Rodriguéz Gonzaléz PS Pereira AM Soares |
author_sort |
RS Fernandes |
title |
Neutralization of pharmacological and toxic activities of Bothrops jararacussu snake venom and isolated myotoxins by Serjania erecta methanolic extract and its fractions |
title_short |
Neutralization of pharmacological and toxic activities of Bothrops jararacussu snake venom and isolated myotoxins by Serjania erecta methanolic extract and its fractions |
title_full |
Neutralization of pharmacological and toxic activities of Bothrops jararacussu snake venom and isolated myotoxins by Serjania erecta methanolic extract and its fractions |
title_fullStr |
Neutralization of pharmacological and toxic activities of Bothrops jararacussu snake venom and isolated myotoxins by Serjania erecta methanolic extract and its fractions |
title_full_unstemmed |
Neutralization of pharmacological and toxic activities of Bothrops jararacussu snake venom and isolated myotoxins by Serjania erecta methanolic extract and its fractions |
title_sort |
neutralization of pharmacological and toxic activities of bothrops jararacussu snake venom and isolated myotoxins by serjania erecta methanolic extract and its fractions |
publisher |
SciELO |
publishDate |
2011 |
url |
https://doi.org/10.1590/S1678-91992011000100011 https://doaj.org/article/437f453f16694a48bcbfacbe9c4f56ac |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Journal of Venomous Animals and Toxins including Tropical Diseases, Vol 17, Iss 1, Pp 85-93 (2011) |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992011000100011 https://doaj.org/toc/1678-9199 doi:10.1590/S1678-91992011000100011 1678-9199 https://doaj.org/article/437f453f16694a48bcbfacbe9c4f56ac |
op_doi |
https://doi.org/10.1590/S1678-91992011000100011 |
container_title |
Journal of Venomous Animals and Toxins including Tropical Diseases |
container_volume |
17 |
container_issue |
1 |
container_start_page |
85 |
op_container_end_page |
93 |
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