Antibacterial, antioxidant, and sun protection potential of selected ethno medicinal plants used for skin infections in Uganda
Abstract Background Rural populations in Uganda rely heavily on medicinal plants for the treatment of bacterial skin infections. However, the efficacy of these medicinal plants for their pharmacological action is not known. The study aimed at evaluating the antibacterial, antioxidant, and sun protec...
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ftdoajarticles:oai:doaj.org/article:437ed5af9f844b9d9e7abebbc311671e 2023-05-15T15:14:42+02:00 Antibacterial, antioxidant, and sun protection potential of selected ethno medicinal plants used for skin infections in Uganda Jane Namukobe Peter Sekandi Robert Byamukama Moses Murungi Jennifer Nambooze Yeremiah Ekyibetenga Christine Betty Nagawa Savina Asiimwe 2021-06-01T00:00:00Z https://doi.org/10.1186/s41182-021-00342-y https://doaj.org/article/437ed5af9f844b9d9e7abebbc311671e EN eng BMC https://doi.org/10.1186/s41182-021-00342-y https://doaj.org/toc/1349-4147 doi:10.1186/s41182-021-00342-y 1349-4147 https://doaj.org/article/437ed5af9f844b9d9e7abebbc311671e Tropical Medicine and Health, Vol 49, Iss 1, Pp 1-10 (2021) Antibacterial Antioxidant Toxicity Sun protection Medicinal plants Skin infections Arctic medicine. Tropical medicine RC955-962 article 2021 ftdoajarticles https://doi.org/10.1186/s41182-021-00342-y 2022-12-31T11:43:22Z Abstract Background Rural populations in Uganda rely heavily on medicinal plants for the treatment of bacterial skin infections. However, the efficacy of these medicinal plants for their pharmacological action is not known. The study aimed at evaluating the antibacterial, antioxidant, and sun protection potential of Spermacoce princeae, Psorospermum febrifugum, Plectranthus caespitosus, and Erlangea tomentosa extracts. Methods The plant samples were extracted by maceration sequentially using hexane, dichloromethane, ethyl acetate, methanol, and distilled water. Antibacterial activity of each extract was carried out using an agar well diffusion assay against Pseudomonas aeruginosa, Staphylococcus aureus, Escherichia coli, Klebsiella pneumonie, Streptococcus pyogenes, and Salmonella typhi. Acute dermal toxicity of the aqueous extract of S. princeae and P. febrifugum, and E. tomentosa was assessed in young adult healthy Wistar albino rats at a dose of 8000 and 10,000 mg/kg body weight. The antioxidant activity of each extract was carried out using a 1,1-diphenyl-2-picryl-hydrazyl (DPPH) radical scavenging assay. The sun protection factor was determined using Shimadzu UltraViolet-Visible double beam spectrophotometer between 290 and 320 nm. Results The plant extracts showed good antibacterial activity against the tested bacterial strains with minimum inhibitory concentration (MIC) ranging between 3.12 and 12.5 mg/ml. There was no significant change in the levels of creatinine, alanine aminotransferase, and aspartate aminotransferase in the rats even at a higher dose of 10,000 mg/kg, which was related to the results of biochemical analysis of the blood samples from the treated and control groups. The aqueous and methanol extracts of S. princeae showed potential antioxidant properties, with half maximal inhibitory concentration (IC50) values of 59.82 and 61.20 μg/ml respectively. The organic and aqueous extracts of P. caespitosus showed high levels of protection against Ultraviolet light with sun protection potential ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Tropical Medicine and Health 49 1 |
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Open Polar |
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ftdoajarticles |
language |
English |
topic |
Antibacterial Antioxidant Toxicity Sun protection Medicinal plants Skin infections Arctic medicine. Tropical medicine RC955-962 |
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Antibacterial Antioxidant Toxicity Sun protection Medicinal plants Skin infections Arctic medicine. Tropical medicine RC955-962 Jane Namukobe Peter Sekandi Robert Byamukama Moses Murungi Jennifer Nambooze Yeremiah Ekyibetenga Christine Betty Nagawa Savina Asiimwe Antibacterial, antioxidant, and sun protection potential of selected ethno medicinal plants used for skin infections in Uganda |
topic_facet |
Antibacterial Antioxidant Toxicity Sun protection Medicinal plants Skin infections Arctic medicine. Tropical medicine RC955-962 |
description |
Abstract Background Rural populations in Uganda rely heavily on medicinal plants for the treatment of bacterial skin infections. However, the efficacy of these medicinal plants for their pharmacological action is not known. The study aimed at evaluating the antibacterial, antioxidant, and sun protection potential of Spermacoce princeae, Psorospermum febrifugum, Plectranthus caespitosus, and Erlangea tomentosa extracts. Methods The plant samples were extracted by maceration sequentially using hexane, dichloromethane, ethyl acetate, methanol, and distilled water. Antibacterial activity of each extract was carried out using an agar well diffusion assay against Pseudomonas aeruginosa, Staphylococcus aureus, Escherichia coli, Klebsiella pneumonie, Streptococcus pyogenes, and Salmonella typhi. Acute dermal toxicity of the aqueous extract of S. princeae and P. febrifugum, and E. tomentosa was assessed in young adult healthy Wistar albino rats at a dose of 8000 and 10,000 mg/kg body weight. The antioxidant activity of each extract was carried out using a 1,1-diphenyl-2-picryl-hydrazyl (DPPH) radical scavenging assay. The sun protection factor was determined using Shimadzu UltraViolet-Visible double beam spectrophotometer between 290 and 320 nm. Results The plant extracts showed good antibacterial activity against the tested bacterial strains with minimum inhibitory concentration (MIC) ranging between 3.12 and 12.5 mg/ml. There was no significant change in the levels of creatinine, alanine aminotransferase, and aspartate aminotransferase in the rats even at a higher dose of 10,000 mg/kg, which was related to the results of biochemical analysis of the blood samples from the treated and control groups. The aqueous and methanol extracts of S. princeae showed potential antioxidant properties, with half maximal inhibitory concentration (IC50) values of 59.82 and 61.20 μg/ml respectively. The organic and aqueous extracts of P. caespitosus showed high levels of protection against Ultraviolet light with sun protection potential ... |
format |
Article in Journal/Newspaper |
author |
Jane Namukobe Peter Sekandi Robert Byamukama Moses Murungi Jennifer Nambooze Yeremiah Ekyibetenga Christine Betty Nagawa Savina Asiimwe |
author_facet |
Jane Namukobe Peter Sekandi Robert Byamukama Moses Murungi Jennifer Nambooze Yeremiah Ekyibetenga Christine Betty Nagawa Savina Asiimwe |
author_sort |
Jane Namukobe |
title |
Antibacterial, antioxidant, and sun protection potential of selected ethno medicinal plants used for skin infections in Uganda |
title_short |
Antibacterial, antioxidant, and sun protection potential of selected ethno medicinal plants used for skin infections in Uganda |
title_full |
Antibacterial, antioxidant, and sun protection potential of selected ethno medicinal plants used for skin infections in Uganda |
title_fullStr |
Antibacterial, antioxidant, and sun protection potential of selected ethno medicinal plants used for skin infections in Uganda |
title_full_unstemmed |
Antibacterial, antioxidant, and sun protection potential of selected ethno medicinal plants used for skin infections in Uganda |
title_sort |
antibacterial, antioxidant, and sun protection potential of selected ethno medicinal plants used for skin infections in uganda |
publisher |
BMC |
publishDate |
2021 |
url |
https://doi.org/10.1186/s41182-021-00342-y https://doaj.org/article/437ed5af9f844b9d9e7abebbc311671e |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Tropical Medicine and Health, Vol 49, Iss 1, Pp 1-10 (2021) |
op_relation |
https://doi.org/10.1186/s41182-021-00342-y https://doaj.org/toc/1349-4147 doi:10.1186/s41182-021-00342-y 1349-4147 https://doaj.org/article/437ed5af9f844b9d9e7abebbc311671e |
op_doi |
https://doi.org/10.1186/s41182-021-00342-y |
container_title |
Tropical Medicine and Health |
container_volume |
49 |
container_issue |
1 |
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1766345129015115776 |