Molecular epidemiology of HIV-1 strains in the south-east and east of Turkey
Objective: To detect the subtype characterization and drug-resistant mutations in HIV-1 strains after the refugee movement from Syria to Turkey between 2011 and 2014 in south east border lines. Methods: A total of 65 patients were included in this study, of which 57 (88%) patients were antiretrovira...
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Wolters Kluwer Medknow Publications
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ftdoajarticles:oai:doaj.org/article:4363f40052254543aaf3441173502884 2023-05-15T15:13:18+02:00 Molecular epidemiology of HIV-1 strains in the south-east and east of Turkey Mustafa Kemal Çelen Murat Sayan Tuba Dal Celal Ayaz Alicem Tekin Tuncer Özekinci Suda Tekin Koruk Tunga Barcin Recep Tekin Mehmet Sinan Dal Sevgi Kalkanlı 2015-09-01T00:00:00Z https://doi.org/10.1016/j.apjtb.2015.07.009 https://doaj.org/article/4363f40052254543aaf3441173502884 EN eng Wolters Kluwer Medknow Publications http://www.sciencedirect.com/science/article/pii/S222116911500146X https://doaj.org/toc/2221-1691 2221-1691 doi:10.1016/j.apjtb.2015.07.009 https://doaj.org/article/4363f40052254543aaf3441173502884 Asian Pacific Journal of Tropical Biomedicine, Vol 5, Iss 9, Pp 773-777 (2015) HIV-1 Turkey Molecular epidemiology Refugee movement Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 article 2015 ftdoajarticles https://doi.org/10.1016/j.apjtb.2015.07.009 2022-12-31T15:22:15Z Objective: To detect the subtype characterization and drug-resistant mutations in HIV-1 strains after the refugee movement from Syria to Turkey between 2011 and 2014 in south east border lines. Methods: A total of 65 patients were included in this study, of which 57 (88%) patients were antiretroviral therapy-naive patients. HIV-1 RNA was detected and quantified by real-time PCR assay. HIV-1 subtypes and circulating recombinant forms (CRFs) were identified by phylogenetic analysis (neighbor-joining method), and drug-resistant mutations were analyzed. Results: Three major HIV groups were indicated. Two of these groups were located in subtype B. The other group showed heterogeneity. Subtype B (48/65, 73.8%), followed by CRFs (12/65, 18.5%) was the most common strain. Subtype of CRFs consisted of CRF01_AE (9/65, 13.8%) and CRF02_AG (3/65, 4.6%). Subtype C (1/65, 1.5%), sub-subtypes A1 (2/65, 3.1%) and F1 (2/65, 3.1%) were also detected with low prevalence. The rate of overall primary antiretroviral resistance was 4.9% (3/61). Drug-resistant rate for non-nucleoside reverse transcriptase inhibitors was 4.9%. The thymidine analogue mutation rate was 13.1% (8/61). Conclusions: HIV molecular epidemiology studies are necessary to determine transmission patterns and spread. Subtype B and CRF01_AE, CRF02_AG are the most prevalent strains in the south-east of Turkey. However, subtype C, sub-subtypes A1 and F1 are of low prevalence but persist in the south-east of Turkey. In the near future, changing of HIV epidemiology will be possible in Turkey due to migration movement in border lines and resistance testing will play an important role in HIV management. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Asian Pacific Journal of Tropical Biomedicine 5 9 773 777 |
institution |
Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
HIV-1 Turkey Molecular epidemiology Refugee movement Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 |
spellingShingle |
HIV-1 Turkey Molecular epidemiology Refugee movement Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 Mustafa Kemal Çelen Murat Sayan Tuba Dal Celal Ayaz Alicem Tekin Tuncer Özekinci Suda Tekin Koruk Tunga Barcin Recep Tekin Mehmet Sinan Dal Sevgi Kalkanlı Molecular epidemiology of HIV-1 strains in the south-east and east of Turkey |
topic_facet |
HIV-1 Turkey Molecular epidemiology Refugee movement Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 |
description |
Objective: To detect the subtype characterization and drug-resistant mutations in HIV-1 strains after the refugee movement from Syria to Turkey between 2011 and 2014 in south east border lines. Methods: A total of 65 patients were included in this study, of which 57 (88%) patients were antiretroviral therapy-naive patients. HIV-1 RNA was detected and quantified by real-time PCR assay. HIV-1 subtypes and circulating recombinant forms (CRFs) were identified by phylogenetic analysis (neighbor-joining method), and drug-resistant mutations were analyzed. Results: Three major HIV groups were indicated. Two of these groups were located in subtype B. The other group showed heterogeneity. Subtype B (48/65, 73.8%), followed by CRFs (12/65, 18.5%) was the most common strain. Subtype of CRFs consisted of CRF01_AE (9/65, 13.8%) and CRF02_AG (3/65, 4.6%). Subtype C (1/65, 1.5%), sub-subtypes A1 (2/65, 3.1%) and F1 (2/65, 3.1%) were also detected with low prevalence. The rate of overall primary antiretroviral resistance was 4.9% (3/61). Drug-resistant rate for non-nucleoside reverse transcriptase inhibitors was 4.9%. The thymidine analogue mutation rate was 13.1% (8/61). Conclusions: HIV molecular epidemiology studies are necessary to determine transmission patterns and spread. Subtype B and CRF01_AE, CRF02_AG are the most prevalent strains in the south-east of Turkey. However, subtype C, sub-subtypes A1 and F1 are of low prevalence but persist in the south-east of Turkey. In the near future, changing of HIV epidemiology will be possible in Turkey due to migration movement in border lines and resistance testing will play an important role in HIV management. |
format |
Article in Journal/Newspaper |
author |
Mustafa Kemal Çelen Murat Sayan Tuba Dal Celal Ayaz Alicem Tekin Tuncer Özekinci Suda Tekin Koruk Tunga Barcin Recep Tekin Mehmet Sinan Dal Sevgi Kalkanlı |
author_facet |
Mustafa Kemal Çelen Murat Sayan Tuba Dal Celal Ayaz Alicem Tekin Tuncer Özekinci Suda Tekin Koruk Tunga Barcin Recep Tekin Mehmet Sinan Dal Sevgi Kalkanlı |
author_sort |
Mustafa Kemal Çelen |
title |
Molecular epidemiology of HIV-1 strains in the south-east and east of Turkey |
title_short |
Molecular epidemiology of HIV-1 strains in the south-east and east of Turkey |
title_full |
Molecular epidemiology of HIV-1 strains in the south-east and east of Turkey |
title_fullStr |
Molecular epidemiology of HIV-1 strains in the south-east and east of Turkey |
title_full_unstemmed |
Molecular epidemiology of HIV-1 strains in the south-east and east of Turkey |
title_sort |
molecular epidemiology of hiv-1 strains in the south-east and east of turkey |
publisher |
Wolters Kluwer Medknow Publications |
publishDate |
2015 |
url |
https://doi.org/10.1016/j.apjtb.2015.07.009 https://doaj.org/article/4363f40052254543aaf3441173502884 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Asian Pacific Journal of Tropical Biomedicine, Vol 5, Iss 9, Pp 773-777 (2015) |
op_relation |
http://www.sciencedirect.com/science/article/pii/S222116911500146X https://doaj.org/toc/2221-1691 2221-1691 doi:10.1016/j.apjtb.2015.07.009 https://doaj.org/article/4363f40052254543aaf3441173502884 |
op_doi |
https://doi.org/10.1016/j.apjtb.2015.07.009 |
container_title |
Asian Pacific Journal of Tropical Biomedicine |
container_volume |
5 |
container_issue |
9 |
container_start_page |
773 |
op_container_end_page |
777 |
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1766343867117862912 |