Synthesis of Candida Antarctica Lipase B (CALB) enzyme-powered magnetite nanomotor based on PCL/Chitosan Janus nanostructure
Abstract In this work, we report the design and synthesis of internal energy-driven Janus nanomotors (JNMs), which are composed of certain reactive materials that are capable of converting chemical energy in the backbone of nanomotors into kinetic energy. For this purpose, superparamagnetic iron oxi...
Published in: | Scientific Reports |
---|---|
Main Authors: | , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Nature Portfolio
2022
|
Subjects: | |
Online Access: | https://doi.org/10.1038/s41598-022-16777-0 https://doaj.org/article/4352b54e75714bd5b495393c9af3e5de |
id |
ftdoajarticles:oai:doaj.org/article:4352b54e75714bd5b495393c9af3e5de |
---|---|
record_format |
openpolar |
spelling |
ftdoajarticles:oai:doaj.org/article:4352b54e75714bd5b495393c9af3e5de 2023-05-15T13:40:17+02:00 Synthesis of Candida Antarctica Lipase B (CALB) enzyme-powered magnetite nanomotor based on PCL/Chitosan Janus nanostructure Fariba Mafakheri Sepideh Khoee 2022-07-01T00:00:00Z https://doi.org/10.1038/s41598-022-16777-0 https://doaj.org/article/4352b54e75714bd5b495393c9af3e5de EN eng Nature Portfolio https://doi.org/10.1038/s41598-022-16777-0 https://doaj.org/toc/2045-2322 doi:10.1038/s41598-022-16777-0 2045-2322 https://doaj.org/article/4352b54e75714bd5b495393c9af3e5de Scientific Reports, Vol 12, Iss 1, Pp 1-12 (2022) Medicine R Science Q article 2022 ftdoajarticles https://doi.org/10.1038/s41598-022-16777-0 2022-12-31T00:47:25Z Abstract In this work, we report the design and synthesis of internal energy-driven Janus nanomotors (JNMs), which are composed of certain reactive materials that are capable of converting chemical energy in the backbone of nanomotors into kinetic energy. For this purpose, superparamagnetic iron oxide nanoparticles (SPIONs) with the anisotropic surface were obtained via a Pickering emulsion. Modified chitosan (as hydrophilic polymer) and functionalized polycaprolactone (as hydrophobic domain) were covalently linked to the surface of bi-functional SPIONs to produce Janus nanoparticles (JNPs). Then, the CALB enzyme was loaded in the PCL hemisphere of JNPs to form the Janus nanomotor. When nanomotors are placed in the phosphate-buffered saline solution, the driving force for motion is provided by the decomposition of polyester into monomers and oligomers on one side of the JNMs. The trajectories of the nanomotors were recorded under different circumstances by a video microscope and analyzed by the mean squared displacement. The results show that the velocity of JNMs increases with an increasing percentage of the loaded enzyme. In addition, the diffusion coefficient enhances up to 87.67% in compared with nanoparticles without enzyme. Controlling the motion direction of JNMs by an external magnetic field is also possible, due to the presence of SPIONs. Article in Journal/Newspaper Antarc* Antarctica Directory of Open Access Journals: DOAJ Articles Janus ENVELOPE(163.100,163.100,-71.067,-71.067) Scientific Reports 12 1 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Medicine R Science Q |
spellingShingle |
Medicine R Science Q Fariba Mafakheri Sepideh Khoee Synthesis of Candida Antarctica Lipase B (CALB) enzyme-powered magnetite nanomotor based on PCL/Chitosan Janus nanostructure |
topic_facet |
Medicine R Science Q |
description |
Abstract In this work, we report the design and synthesis of internal energy-driven Janus nanomotors (JNMs), which are composed of certain reactive materials that are capable of converting chemical energy in the backbone of nanomotors into kinetic energy. For this purpose, superparamagnetic iron oxide nanoparticles (SPIONs) with the anisotropic surface were obtained via a Pickering emulsion. Modified chitosan (as hydrophilic polymer) and functionalized polycaprolactone (as hydrophobic domain) were covalently linked to the surface of bi-functional SPIONs to produce Janus nanoparticles (JNPs). Then, the CALB enzyme was loaded in the PCL hemisphere of JNPs to form the Janus nanomotor. When nanomotors are placed in the phosphate-buffered saline solution, the driving force for motion is provided by the decomposition of polyester into monomers and oligomers on one side of the JNMs. The trajectories of the nanomotors were recorded under different circumstances by a video microscope and analyzed by the mean squared displacement. The results show that the velocity of JNMs increases with an increasing percentage of the loaded enzyme. In addition, the diffusion coefficient enhances up to 87.67% in compared with nanoparticles without enzyme. Controlling the motion direction of JNMs by an external magnetic field is also possible, due to the presence of SPIONs. |
format |
Article in Journal/Newspaper |
author |
Fariba Mafakheri Sepideh Khoee |
author_facet |
Fariba Mafakheri Sepideh Khoee |
author_sort |
Fariba Mafakheri |
title |
Synthesis of Candida Antarctica Lipase B (CALB) enzyme-powered magnetite nanomotor based on PCL/Chitosan Janus nanostructure |
title_short |
Synthesis of Candida Antarctica Lipase B (CALB) enzyme-powered magnetite nanomotor based on PCL/Chitosan Janus nanostructure |
title_full |
Synthesis of Candida Antarctica Lipase B (CALB) enzyme-powered magnetite nanomotor based on PCL/Chitosan Janus nanostructure |
title_fullStr |
Synthesis of Candida Antarctica Lipase B (CALB) enzyme-powered magnetite nanomotor based on PCL/Chitosan Janus nanostructure |
title_full_unstemmed |
Synthesis of Candida Antarctica Lipase B (CALB) enzyme-powered magnetite nanomotor based on PCL/Chitosan Janus nanostructure |
title_sort |
synthesis of candida antarctica lipase b (calb) enzyme-powered magnetite nanomotor based on pcl/chitosan janus nanostructure |
publisher |
Nature Portfolio |
publishDate |
2022 |
url |
https://doi.org/10.1038/s41598-022-16777-0 https://doaj.org/article/4352b54e75714bd5b495393c9af3e5de |
long_lat |
ENVELOPE(163.100,163.100,-71.067,-71.067) |
geographic |
Janus |
geographic_facet |
Janus |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_source |
Scientific Reports, Vol 12, Iss 1, Pp 1-12 (2022) |
op_relation |
https://doi.org/10.1038/s41598-022-16777-0 https://doaj.org/toc/2045-2322 doi:10.1038/s41598-022-16777-0 2045-2322 https://doaj.org/article/4352b54e75714bd5b495393c9af3e5de |
op_doi |
https://doi.org/10.1038/s41598-022-16777-0 |
container_title |
Scientific Reports |
container_volume |
12 |
container_issue |
1 |
_version_ |
1766131814733185024 |