The first clawed lobster virus Homarus gammarus nudivirus (HgNV n. sp.) expands the diversity of the Nudiviridae
Abstract Viral diseases of crustaceans are increasingly recognised as challenges to shellfish farms and fisheries. Here we describe the first naturally-occurring virus reported in any clawed lobster species. Hypertrophied nuclei with emarginated chromatin, characteristic histopathological lesions of...
Published in: | Scientific Reports |
---|---|
Main Authors: | , , , , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Nature Portfolio
2019
|
Subjects: | |
Online Access: | https://doi.org/10.1038/s41598-019-46008-y https://doaj.org/article/42e57a828ebd4c5d88a67ffd3dddb29d |
id |
ftdoajarticles:oai:doaj.org/article:42e57a828ebd4c5d88a67ffd3dddb29d |
---|---|
record_format |
openpolar |
spelling |
ftdoajarticles:oai:doaj.org/article:42e57a828ebd4c5d88a67ffd3dddb29d 2023-05-15T16:34:42+02:00 The first clawed lobster virus Homarus gammarus nudivirus (HgNV n. sp.) expands the diversity of the Nudiviridae Corey C. Holt Michelle Stone David Bass Kelly S. Bateman Ronny van Aerle Carly L. Daniels Mark van der Giezen Stuart H. Ross Chantelle Hooper Grant D. Stentiford 2019-07-01T00:00:00Z https://doi.org/10.1038/s41598-019-46008-y https://doaj.org/article/42e57a828ebd4c5d88a67ffd3dddb29d EN eng Nature Portfolio https://doi.org/10.1038/s41598-019-46008-y https://doaj.org/toc/2045-2322 doi:10.1038/s41598-019-46008-y 2045-2322 https://doaj.org/article/42e57a828ebd4c5d88a67ffd3dddb29d Scientific Reports, Vol 9, Iss 1, Pp 1-15 (2019) Medicine R Science Q article 2019 ftdoajarticles https://doi.org/10.1038/s41598-019-46008-y 2022-12-31T13:56:24Z Abstract Viral diseases of crustaceans are increasingly recognised as challenges to shellfish farms and fisheries. Here we describe the first naturally-occurring virus reported in any clawed lobster species. Hypertrophied nuclei with emarginated chromatin, characteristic histopathological lesions of DNA virus infection, were observed within the hepatopancreatic epithelial cells of juvenile European lobsters (Homarus gammarus). Transmission electron microscopy revealed infection with a bacilliform virus containing a rod shaped nucleocapsid enveloped in an elliptical membrane. Assembly of PCR-free shotgun metagenomic sequencing produced a circular genome of 107,063 bp containing 97 open reading frames, the majority of which share sequence similarity with a virus infecting the black tiger shrimp: Penaeus monodon nudivirus (PmNV). Multiple phylogenetic analyses confirm the new virus to be a novel member of the Nudiviridae: Homarus gammarus nudivirus (HgNV). Evidence of occlusion body formation, characteristic of PmNV and its closest relatives, was not observed, questioning the horizontal transmission strategy of HgNV outside of the host. We discuss the potential impacts of HgNV on juvenile lobster growth and mortality and present HgNV-specific primers to serve as a diagnostic tool for monitoring the virus in wild and farmed lobster stocks. Article in Journal/Newspaper Homarus gammarus Directory of Open Access Journals: DOAJ Articles Scientific Reports 9 1 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Medicine R Science Q |
spellingShingle |
Medicine R Science Q Corey C. Holt Michelle Stone David Bass Kelly S. Bateman Ronny van Aerle Carly L. Daniels Mark van der Giezen Stuart H. Ross Chantelle Hooper Grant D. Stentiford The first clawed lobster virus Homarus gammarus nudivirus (HgNV n. sp.) expands the diversity of the Nudiviridae |
topic_facet |
Medicine R Science Q |
description |
Abstract Viral diseases of crustaceans are increasingly recognised as challenges to shellfish farms and fisheries. Here we describe the first naturally-occurring virus reported in any clawed lobster species. Hypertrophied nuclei with emarginated chromatin, characteristic histopathological lesions of DNA virus infection, were observed within the hepatopancreatic epithelial cells of juvenile European lobsters (Homarus gammarus). Transmission electron microscopy revealed infection with a bacilliform virus containing a rod shaped nucleocapsid enveloped in an elliptical membrane. Assembly of PCR-free shotgun metagenomic sequencing produced a circular genome of 107,063 bp containing 97 open reading frames, the majority of which share sequence similarity with a virus infecting the black tiger shrimp: Penaeus monodon nudivirus (PmNV). Multiple phylogenetic analyses confirm the new virus to be a novel member of the Nudiviridae: Homarus gammarus nudivirus (HgNV). Evidence of occlusion body formation, characteristic of PmNV and its closest relatives, was not observed, questioning the horizontal transmission strategy of HgNV outside of the host. We discuss the potential impacts of HgNV on juvenile lobster growth and mortality and present HgNV-specific primers to serve as a diagnostic tool for monitoring the virus in wild and farmed lobster stocks. |
format |
Article in Journal/Newspaper |
author |
Corey C. Holt Michelle Stone David Bass Kelly S. Bateman Ronny van Aerle Carly L. Daniels Mark van der Giezen Stuart H. Ross Chantelle Hooper Grant D. Stentiford |
author_facet |
Corey C. Holt Michelle Stone David Bass Kelly S. Bateman Ronny van Aerle Carly L. Daniels Mark van der Giezen Stuart H. Ross Chantelle Hooper Grant D. Stentiford |
author_sort |
Corey C. Holt |
title |
The first clawed lobster virus Homarus gammarus nudivirus (HgNV n. sp.) expands the diversity of the Nudiviridae |
title_short |
The first clawed lobster virus Homarus gammarus nudivirus (HgNV n. sp.) expands the diversity of the Nudiviridae |
title_full |
The first clawed lobster virus Homarus gammarus nudivirus (HgNV n. sp.) expands the diversity of the Nudiviridae |
title_fullStr |
The first clawed lobster virus Homarus gammarus nudivirus (HgNV n. sp.) expands the diversity of the Nudiviridae |
title_full_unstemmed |
The first clawed lobster virus Homarus gammarus nudivirus (HgNV n. sp.) expands the diversity of the Nudiviridae |
title_sort |
first clawed lobster virus homarus gammarus nudivirus (hgnv n. sp.) expands the diversity of the nudiviridae |
publisher |
Nature Portfolio |
publishDate |
2019 |
url |
https://doi.org/10.1038/s41598-019-46008-y https://doaj.org/article/42e57a828ebd4c5d88a67ffd3dddb29d |
genre |
Homarus gammarus |
genre_facet |
Homarus gammarus |
op_source |
Scientific Reports, Vol 9, Iss 1, Pp 1-15 (2019) |
op_relation |
https://doi.org/10.1038/s41598-019-46008-y https://doaj.org/toc/2045-2322 doi:10.1038/s41598-019-46008-y 2045-2322 https://doaj.org/article/42e57a828ebd4c5d88a67ffd3dddb29d |
op_doi |
https://doi.org/10.1038/s41598-019-46008-y |
container_title |
Scientific Reports |
container_volume |
9 |
container_issue |
1 |
_version_ |
1766024674123186176 |