TAXONOMIC STRUCTURE OF PROKARYOTIC COMMUNITIES IN SOILS OF DIFFERENT BIOCLIMATIC ZONES

• Published in: Dokuchaev Soil Bulletin
• Main Authors: I. A. Tikhonovich, T. I. Chernov, A. D. Zhelezova, A. K. Tkhakakhova, E. E. Andronov, O. V. Kutovaya
• Format: Article in Journal/Newspaper
• Language: Russian
• Published: V.V. Dokuchaev Soil Science Institute 2018
• Subjects: next-generation sequencing; profile distribution of microorganisms; bacteria; archaea; sod-podzolic soil; dark gray soil; chernozem; brown soil; chestnut soil; solonets; Agriculture (General); S1-972; taiga
• Online Access: https://doi.org/10.19047/0136-1694-2018-95-125-153; https://doaj.org/article/42d070ece3db468eb8824a4d0424c6b0

Using high-throughput sequencing, we performed a full-profile analysis of the taxonomic structure and diversity of prokaryotic communities of zonal soil series: sod-podzolic, dark gray, typical chernozem, brown soil, meadow-chestnut soil and solonets. Phyla Acidobacteria, Actinobacteria, Bacteroidetes, Chloroflexi, Firmicutes, Gemmatimonadetes, Planctomycetes, Proteobacteria and Verrucomicrobia formed up to 95% of prokaryotic communities in all studied soil horizons. Phyla Proteobacteria and Actinobacteria and archaeal phylum Thaumarchaeota dominated in all soils. We revealed the trends of changes in the representation of bacterial phyla in the soils of the zonal range from taiga to dry-steppe: a decrease of Acidobacteria and an increase of Actinobacteria in the upper horizons. The diversity indices of the prokaryotic communities of different genetic horizons of the studied soils were evaluated. In all soils, a decrease in diversity indices with the depth of the horizon was revealed. In all soils, a decrease in diversity indices with depth of the horizon was found, with a rare and slight increase in some lower horizons: structural metamorphic (BM), textural (BT) or accumulative carbonate (BCA) horizons. When examining the prokaryotic communities of soils with different genesis, no determining influence of pH and organic matter contents on the diversity indices was found. Differences in the taxonomic structure and diversity of prokaryotic communities of soils of different bioclimatic zones were presented due to a combination of factors: the difference in chemical and physical properties of soils, as well as in water and temperature conditions.