Genetic characterization of clinically relevant class 1 integrons carried by multidrug resistant bacteria (MDRB) isolated from the gut microbiota of highly antibiotic treated Salmo salar
ABSTRACT: Objectives: The main objective of this study was the genetic characterization of clinically relevant class 1 integrons carried by multidrug resistant bacteria isolated from the intestinal microbiota of aquaculture salmon treated with high concentrations of antibiotics. Methods: In 82 multi...
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ftdoajarticles:oai:doaj.org/article:42cf8b69c20c4dbea94d90a336bb2bd3 2023-05-15T18:09:58+02:00 Genetic characterization of clinically relevant class 1 integrons carried by multidrug resistant bacteria (MDRB) isolated from the gut microbiota of highly antibiotic treated Salmo salar Felipe Vásquez-Ponce Sebastián Higuera-Llantén Juan Parás-Silva Nicolás Gamboa-Acuña Jimena Cortés Andrés Opazo-Capurro Juan A. Ugalde Manuel Alcalde-Rico Jorge Olivares-Pacheco 2022-06-01T00:00:00Z https://doi.org/10.1016/j.jgar.2022.02.003 https://doaj.org/article/42cf8b69c20c4dbea94d90a336bb2bd3 EN eng Elsevier http://www.sciencedirect.com/science/article/pii/S2213716522000364 https://doaj.org/toc/2213-7165 2213-7165 doi:10.1016/j.jgar.2022.02.003 https://doaj.org/article/42cf8b69c20c4dbea94d90a336bb2bd3 Journal of Global Antimicrobial Resistance, Vol 29, Iss , Pp 55-62 (2022) Antimicrobial resistance Class 1 integron Salmon farms Gut microbiota Microbiology QR1-502 article 2022 ftdoajarticles https://doi.org/10.1016/j.jgar.2022.02.003 2022-12-31T02:55:57Z ABSTRACT: Objectives: The main objective of this study was the genetic characterization of clinically relevant class 1 integrons carried by multidrug resistant bacteria isolated from the intestinal microbiota of aquaculture salmon treated with high concentrations of antibiotics. Methods: In 82 multidrug resistant bacterial isolates, the prevalence of both the conserved elements of the integrons, qacEΔ1 and sul1 genes, and the variable region (VR) was determined. Further, whole genome sequencing and complete genetic analysis was performed in VR-positive isolates. Results: Despite the fact that 100% of the bacterial isolates presented the intI1 gene, only 12.3% carried the qacEΔ1 and sul1 genes and only two (2.4%) presented a VR with gene cassettes. In the Pseudomonas baetica 25P2F9 isolate, a VR carrying aac(6’)31, qacH, and blaOXA-2 gene cassettes was described, whereas the VR of Aeromonas salmonicida 30PB8 isolate showed a dfrA14 gene cassette. The array of gene cassettes found in the Pseudomonas isolate appears with high frequency in clinically relevant pathogens such as Pseudomonas aeruginosa or Escherichia coli. Additionally, it was possible to determine that these integrons are contained in plasmids and coul be easily transferred. Resistome analysis demonstrated that both isolates carried a great diversity of antibiotic resistance genes, including many β-lactamases. Even in the Aeromonas isolate a new oxacillin-hydrolyzing beta-lactamase gene was described (blaOXA-956). Conclusion: The presence of multidrug resistant bacteria and clinically relevant genetic elements in the salmon intestinal microbiota make the aquaculture a hotspot in the phenomenon of antibiotic resistance; therefore, the control of antibiotics used in this activity is a key point to avoid its escalation. Article in Journal/Newspaper Salmo salar Directory of Open Access Journals: DOAJ Articles Journal of Global Antimicrobial Resistance 29 55 62 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Antimicrobial resistance Class 1 integron Salmon farms Gut microbiota Microbiology QR1-502 |
spellingShingle |
Antimicrobial resistance Class 1 integron Salmon farms Gut microbiota Microbiology QR1-502 Felipe Vásquez-Ponce Sebastián Higuera-Llantén Juan Parás-Silva Nicolás Gamboa-Acuña Jimena Cortés Andrés Opazo-Capurro Juan A. Ugalde Manuel Alcalde-Rico Jorge Olivares-Pacheco Genetic characterization of clinically relevant class 1 integrons carried by multidrug resistant bacteria (MDRB) isolated from the gut microbiota of highly antibiotic treated Salmo salar |
topic_facet |
Antimicrobial resistance Class 1 integron Salmon farms Gut microbiota Microbiology QR1-502 |
description |
ABSTRACT: Objectives: The main objective of this study was the genetic characterization of clinically relevant class 1 integrons carried by multidrug resistant bacteria isolated from the intestinal microbiota of aquaculture salmon treated with high concentrations of antibiotics. Methods: In 82 multidrug resistant bacterial isolates, the prevalence of both the conserved elements of the integrons, qacEΔ1 and sul1 genes, and the variable region (VR) was determined. Further, whole genome sequencing and complete genetic analysis was performed in VR-positive isolates. Results: Despite the fact that 100% of the bacterial isolates presented the intI1 gene, only 12.3% carried the qacEΔ1 and sul1 genes and only two (2.4%) presented a VR with gene cassettes. In the Pseudomonas baetica 25P2F9 isolate, a VR carrying aac(6’)31, qacH, and blaOXA-2 gene cassettes was described, whereas the VR of Aeromonas salmonicida 30PB8 isolate showed a dfrA14 gene cassette. The array of gene cassettes found in the Pseudomonas isolate appears with high frequency in clinically relevant pathogens such as Pseudomonas aeruginosa or Escherichia coli. Additionally, it was possible to determine that these integrons are contained in plasmids and coul be easily transferred. Resistome analysis demonstrated that both isolates carried a great diversity of antibiotic resistance genes, including many β-lactamases. Even in the Aeromonas isolate a new oxacillin-hydrolyzing beta-lactamase gene was described (blaOXA-956). Conclusion: The presence of multidrug resistant bacteria and clinically relevant genetic elements in the salmon intestinal microbiota make the aquaculture a hotspot in the phenomenon of antibiotic resistance; therefore, the control of antibiotics used in this activity is a key point to avoid its escalation. |
format |
Article in Journal/Newspaper |
author |
Felipe Vásquez-Ponce Sebastián Higuera-Llantén Juan Parás-Silva Nicolás Gamboa-Acuña Jimena Cortés Andrés Opazo-Capurro Juan A. Ugalde Manuel Alcalde-Rico Jorge Olivares-Pacheco |
author_facet |
Felipe Vásquez-Ponce Sebastián Higuera-Llantén Juan Parás-Silva Nicolás Gamboa-Acuña Jimena Cortés Andrés Opazo-Capurro Juan A. Ugalde Manuel Alcalde-Rico Jorge Olivares-Pacheco |
author_sort |
Felipe Vásquez-Ponce |
title |
Genetic characterization of clinically relevant class 1 integrons carried by multidrug resistant bacteria (MDRB) isolated from the gut microbiota of highly antibiotic treated Salmo salar |
title_short |
Genetic characterization of clinically relevant class 1 integrons carried by multidrug resistant bacteria (MDRB) isolated from the gut microbiota of highly antibiotic treated Salmo salar |
title_full |
Genetic characterization of clinically relevant class 1 integrons carried by multidrug resistant bacteria (MDRB) isolated from the gut microbiota of highly antibiotic treated Salmo salar |
title_fullStr |
Genetic characterization of clinically relevant class 1 integrons carried by multidrug resistant bacteria (MDRB) isolated from the gut microbiota of highly antibiotic treated Salmo salar |
title_full_unstemmed |
Genetic characterization of clinically relevant class 1 integrons carried by multidrug resistant bacteria (MDRB) isolated from the gut microbiota of highly antibiotic treated Salmo salar |
title_sort |
genetic characterization of clinically relevant class 1 integrons carried by multidrug resistant bacteria (mdrb) isolated from the gut microbiota of highly antibiotic treated salmo salar |
publisher |
Elsevier |
publishDate |
2022 |
url |
https://doi.org/10.1016/j.jgar.2022.02.003 https://doaj.org/article/42cf8b69c20c4dbea94d90a336bb2bd3 |
genre |
Salmo salar |
genre_facet |
Salmo salar |
op_source |
Journal of Global Antimicrobial Resistance, Vol 29, Iss , Pp 55-62 (2022) |
op_relation |
http://www.sciencedirect.com/science/article/pii/S2213716522000364 https://doaj.org/toc/2213-7165 2213-7165 doi:10.1016/j.jgar.2022.02.003 https://doaj.org/article/42cf8b69c20c4dbea94d90a336bb2bd3 |
op_doi |
https://doi.org/10.1016/j.jgar.2022.02.003 |
container_title |
Journal of Global Antimicrobial Resistance |
container_volume |
29 |
container_start_page |
55 |
op_container_end_page |
62 |
_version_ |
1766182677095907328 |