Kinetic modeling of Shewanella baltica KB30 growth on different substrates through respirometry

• Published in: Microbial Cell Factories
• Main Authors: Juan Carlos Leyva-Díaz, José Manuel Poyatos, Paolo Barghini, Susanna Gorrasi, Massimiliano Fenice
• Format: Article in Journal/Newspaper
• Language: English
• Published: BMC 2017
• Subjects: Carbon utilization; Kinetics; Modeling; Shewanella baltica; Microbiology; QR1-502; White Sea; Kandalaksha; Moser
• Online Access: https://doi.org/10.1186/s12934-017-0805-7; https://doaj.org/article/42c32d151a0047b9a77884f2e13bbfe4

Abstract Background Shewanella baltica KB30 was isolated from seawater collected in Kandalaksha Bay, White Sea (Russia). This strain is known for its ability to grow on a pool of different substrates, including carbohydrates, carboxylic and amino acids, and lipids. However, no data are available on its metabolic efficiency in relation to the use of different carbon sources typologies. This work represents the first attempt to characterize S. baltica by its heterotrophic kinetic performance. Results Growth and substrate consumption, during the biodegradation of sodium acetate, glucose, tween 80 and peptone, were analyzed through a respirometric method. To find the model best fitting the experimental data and to obtain the kinetic parameters, the equations of Monod, Moser, Contois and Tessier were applied. The kinetic behavior of S. baltica was fitted to Monod model for sodium acetate and tween 80, while it was adjusted to Contois model for glucose and peptone. In this regard, peptone was consumed faster than the other substrates, as indicated by the highest values of substrate degradation rate, which exceeded 60 mg O2 L−1 h−1. Conclusions Proteolytic metabolism was favored than lipidic and glucidic metabolism, which could contribute much more to mineralization and recycling of proteins than lipids and carbohydrates.