Antigen characterization of vector-borne and cultured metacyclic trypomastigotes of Trypanosoma cruzi

• Published in: Revista do Instituto de Medicina Tropical de São Paulo
• Main Authors: N. Yoshida, M. M. G. Teixeira, C. A. Sbravate
• Format: Article in Journal/Newspaper
• Language: English
• Published: Universidade de São Paulo (USP) 1986
• Subjects: Chagas'disease; vector borne; Trypanosoma cruzi; cultured metacyclic tripomastigotes; Arctic medicine. Tropical medicine; RC955-962; Infectious and parasitic diseases; RC109-216; Arctic
• Online Access: https://doi.org/10.1590/S0036-46651986000200003; https://doaj.org/article/42ac70db27df4742b4e806c7f95f39a9

Metacyclic trypomastigotes ol the CL strain of Trypanosoma cruzi obtained from triatomid vectors and from axenic cultures were comparatively analysed as to their antigen make-up and immunogenic characteristics. They were found to be similar by the various parameters examined. Thus, sera of mice immunized with either one of the two metacyclic types precipitated a 82Kd surface protein from 131I-labeled culture metacyclics. Sera of mice protected against acute T. cruzi infection by immunization with killed culture metacyclics of a different strain (G) recognized, by immunoblotting, a 77Kd protein in both types of CL strain metacyclics. A monoclonal antibody raised against G strain metacyclics, and specific for metacyclic stages of this strain, reacted with both CL strain metacyclic types. Both metacyclic forms were similarly Iysed by various anti-T. cruzi sera, in a complement-mediated reaction.