Biochemical characterization of a phospholipase A2 homologue from the venom of the social wasp Polybia occidentalis
Abstract Background: Wasp venoms constitute a molecular reservoir of new pharmacological substances such as peptides and proteins, biological property holders, many of which are yet to be identified. Exploring these sources may lead to the discovery of molecules hitherto unknown. This study describe...
Published in: | Journal of Venomous Animals and Toxins including Tropical Diseases |
---|---|
Main Authors: | , , , , , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
SciELO
2018
|
Subjects: | |
Online Access: | https://doi.org/10.1186/s40409-018-0143-1 https://doaj.org/article/426dcbb15370457d818b3af54a870f26 |
id |
ftdoajarticles:oai:doaj.org/article:426dcbb15370457d818b3af54a870f26 |
---|---|
record_format |
openpolar |
spelling |
ftdoajarticles:oai:doaj.org/article:426dcbb15370457d818b3af54a870f26 2023-05-15T15:07:43+02:00 Biochemical characterization of a phospholipase A2 homologue from the venom of the social wasp Polybia occidentalis Rafaela Diniz-Sousa Anderson M. Kayano Cleópatra A. Caldeira Rodrigo Simões-Silva Marta C. Monteiro Leandro S. Moreira-Dill Fernando P. Grabner Leonardo A. Calderon Juliana P. Zuliani Rodrigo G. Stábeli Andreimar M. Soares 2018-03-01T00:00:00Z https://doi.org/10.1186/s40409-018-0143-1 https://doaj.org/article/426dcbb15370457d818b3af54a870f26 EN eng SciELO http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992018000100302&lng=en&tlng=en https://doaj.org/toc/1678-9199 1678-9199 doi:10.1186/s40409-018-0143-1 https://doaj.org/article/426dcbb15370457d818b3af54a870f26 Journal of Venomous Animals and Toxins including Tropical Diseases, Vol 24, Iss 0 (2018) Wasp Polybia occidentalis PocTX PLA2 homologue Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 article 2018 ftdoajarticles https://doi.org/10.1186/s40409-018-0143-1 2022-12-31T04:12:06Z Abstract Background: Wasp venoms constitute a molecular reservoir of new pharmacological substances such as peptides and proteins, biological property holders, many of which are yet to be identified. Exploring these sources may lead to the discovery of molecules hitherto unknown. This study describes, for the first time in hymenopteran venoms, the identification of an enzymatically inactive phospholipase A2 (PLA2) from the venom of the social wasp Polybia occidentalis. Methods: P. occidentalis venom was fractioned by molecular exclusion and reverse phase chromatography. For the biochemical characterization of the protein, 1D and 2D SDS-PAGE were performed, along with phospholipase activity assays on synthetic substrates, MALDI-TOF mass spectrometry and sequencing by Edman degradation. Results: The protein, called PocTX, was isolated using two chromatographic steps. Based on the phospholipase activity assay, electrophoresis and mass spectrometry, the protein presented a high degree of purity, with a mass of 13,896. 47 Da and a basic pI. After sequencing by the Edman degradation method, it was found that the protein showed a high identity with snake venom PLA2 homologues. Conclusion: This is the first report of an enzymatically inactive PLA2 isolated from wasp venom, similar to snake PLA2 homologues. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Journal of Venomous Animals and Toxins including Tropical Diseases 24 1 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Wasp Polybia occidentalis PocTX PLA2 homologue Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 |
spellingShingle |
Wasp Polybia occidentalis PocTX PLA2 homologue Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 Rafaela Diniz-Sousa Anderson M. Kayano Cleópatra A. Caldeira Rodrigo Simões-Silva Marta C. Monteiro Leandro S. Moreira-Dill Fernando P. Grabner Leonardo A. Calderon Juliana P. Zuliani Rodrigo G. Stábeli Andreimar M. Soares Biochemical characterization of a phospholipase A2 homologue from the venom of the social wasp Polybia occidentalis |
topic_facet |
Wasp Polybia occidentalis PocTX PLA2 homologue Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 |
description |
Abstract Background: Wasp venoms constitute a molecular reservoir of new pharmacological substances such as peptides and proteins, biological property holders, many of which are yet to be identified. Exploring these sources may lead to the discovery of molecules hitherto unknown. This study describes, for the first time in hymenopteran venoms, the identification of an enzymatically inactive phospholipase A2 (PLA2) from the venom of the social wasp Polybia occidentalis. Methods: P. occidentalis venom was fractioned by molecular exclusion and reverse phase chromatography. For the biochemical characterization of the protein, 1D and 2D SDS-PAGE were performed, along with phospholipase activity assays on synthetic substrates, MALDI-TOF mass spectrometry and sequencing by Edman degradation. Results: The protein, called PocTX, was isolated using two chromatographic steps. Based on the phospholipase activity assay, electrophoresis and mass spectrometry, the protein presented a high degree of purity, with a mass of 13,896. 47 Da and a basic pI. After sequencing by the Edman degradation method, it was found that the protein showed a high identity with snake venom PLA2 homologues. Conclusion: This is the first report of an enzymatically inactive PLA2 isolated from wasp venom, similar to snake PLA2 homologues. |
format |
Article in Journal/Newspaper |
author |
Rafaela Diniz-Sousa Anderson M. Kayano Cleópatra A. Caldeira Rodrigo Simões-Silva Marta C. Monteiro Leandro S. Moreira-Dill Fernando P. Grabner Leonardo A. Calderon Juliana P. Zuliani Rodrigo G. Stábeli Andreimar M. Soares |
author_facet |
Rafaela Diniz-Sousa Anderson M. Kayano Cleópatra A. Caldeira Rodrigo Simões-Silva Marta C. Monteiro Leandro S. Moreira-Dill Fernando P. Grabner Leonardo A. Calderon Juliana P. Zuliani Rodrigo G. Stábeli Andreimar M. Soares |
author_sort |
Rafaela Diniz-Sousa |
title |
Biochemical characterization of a phospholipase A2 homologue from the venom of the social wasp Polybia occidentalis |
title_short |
Biochemical characterization of a phospholipase A2 homologue from the venom of the social wasp Polybia occidentalis |
title_full |
Biochemical characterization of a phospholipase A2 homologue from the venom of the social wasp Polybia occidentalis |
title_fullStr |
Biochemical characterization of a phospholipase A2 homologue from the venom of the social wasp Polybia occidentalis |
title_full_unstemmed |
Biochemical characterization of a phospholipase A2 homologue from the venom of the social wasp Polybia occidentalis |
title_sort |
biochemical characterization of a phospholipase a2 homologue from the venom of the social wasp polybia occidentalis |
publisher |
SciELO |
publishDate |
2018 |
url |
https://doi.org/10.1186/s40409-018-0143-1 https://doaj.org/article/426dcbb15370457d818b3af54a870f26 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Journal of Venomous Animals and Toxins including Tropical Diseases, Vol 24, Iss 0 (2018) |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992018000100302&lng=en&tlng=en https://doaj.org/toc/1678-9199 1678-9199 doi:10.1186/s40409-018-0143-1 https://doaj.org/article/426dcbb15370457d818b3af54a870f26 |
op_doi |
https://doi.org/10.1186/s40409-018-0143-1 |
container_title |
Journal of Venomous Animals and Toxins including Tropical Diseases |
container_volume |
24 |
container_issue |
1 |
_version_ |
1766339155840729088 |