In vivo antiplasmodial and in vitro antioxidant properties of stem bark extracts of Haematostaphis barteri
Objective: To evaluate the antimalarial and antioxidant properties of stem bark extracts of Haematostaphis barteri (H. barteri). Methods: The prophylactic activity of the plant was performed by dosing mice with sulfadoxine-pyrimethamine (1.2 mg/kg), aqueous extract (30, 100, 300 mg/kg) and dichlorom...
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Wolters Kluwer Medknow Publications
2015
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ftdoajarticles:oai:doaj.org/article:425c01445c3c4f61863e8f4abfc7ff17 2023-05-15T15:13:27+02:00 In vivo antiplasmodial and in vitro antioxidant properties of stem bark extracts of Haematostaphis barteri Johnson Nyarko Boampong Akua Afryie Karikari Elvis Ofori Ameyaw 2015-06-01T00:00:00Z https://doi.org/10.1016/j.apjtb.2015.02.002 https://doaj.org/article/425c01445c3c4f61863e8f4abfc7ff17 EN eng Wolters Kluwer Medknow Publications http://www.sciencedirect.com/science/article/pii/S2221169115000684 https://doaj.org/toc/2221-1691 2221-1691 doi:10.1016/j.apjtb.2015.02.002 https://doaj.org/article/425c01445c3c4f61863e8f4abfc7ff17 Asian Pacific Journal of Tropical Biomedicine, Vol 5, Iss 6, Pp 446-450 (2015) Malaria Parasitemia Haematostaphis barteri Antioxidants Phytochemicals Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 article 2015 ftdoajarticles https://doi.org/10.1016/j.apjtb.2015.02.002 2022-12-31T03:51:33Z Objective: To evaluate the antimalarial and antioxidant properties of stem bark extracts of Haematostaphis barteri (H. barteri). Methods: The prophylactic activity of the plant was performed by dosing mice with sulfadoxine-pyrimethamine (1.2 mg/kg), aqueous extract (30, 100, 300 mg/kg) and dichloromethane/methanol (D/M) (30, 100, 300 mg/kg) extracts of H. barteri for 3 days. On the 4th day, the mice were inoculated with Plasmodium berghei. The parasite density was estimated for each mouse 72 h post-parasite inoculation. The curative activity of the plant was also performed by inoculating mice with Plasmodium berghei. Three days later, they were treated with artemether-lumefantrine (4 mg/kg), aqueous and D/M extracts of H. barteri stem bark for 5 days. The in vitro antioxidant property of the aqueous extract was determined by using the reducing power, nitric oxide and total antioxidant capacity assays. Results: The aqueous extract exerted significant (P < 0.05) curative and prophylactic antimalarial activities. The D/M extract exhibited significant curative (P < 0.05) but not prophylactic antiplasmodial effect. The aqueous extract exhibited in vitro antioxidant property with IC50's of (0.930 ± 0.021) mg/mL, (0.800 ± 0.001) mg/mL and (0.22 ± 0.05) mg/mL in the total antioxidant capacity, reducing power and nitric oxide assays. Histological assessment of the liver of aqueous and D/M treated animals did not reveal any sign of toxicity. Conclusions: H. barteri is not toxic which exerted significant curative antiplasmodial effects but the prophylactic property was however fraction dependent. The mechanism of the antiplasmodial activity of H. barteri may partly be mediated by its antioxidant property. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Asian Pacific Journal of Tropical Biomedicine 5 6 446 450 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Malaria Parasitemia Haematostaphis barteri Antioxidants Phytochemicals Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 |
spellingShingle |
Malaria Parasitemia Haematostaphis barteri Antioxidants Phytochemicals Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 Johnson Nyarko Boampong Akua Afryie Karikari Elvis Ofori Ameyaw In vivo antiplasmodial and in vitro antioxidant properties of stem bark extracts of Haematostaphis barteri |
topic_facet |
Malaria Parasitemia Haematostaphis barteri Antioxidants Phytochemicals Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 |
description |
Objective: To evaluate the antimalarial and antioxidant properties of stem bark extracts of Haematostaphis barteri (H. barteri). Methods: The prophylactic activity of the plant was performed by dosing mice with sulfadoxine-pyrimethamine (1.2 mg/kg), aqueous extract (30, 100, 300 mg/kg) and dichloromethane/methanol (D/M) (30, 100, 300 mg/kg) extracts of H. barteri for 3 days. On the 4th day, the mice were inoculated with Plasmodium berghei. The parasite density was estimated for each mouse 72 h post-parasite inoculation. The curative activity of the plant was also performed by inoculating mice with Plasmodium berghei. Three days later, they were treated with artemether-lumefantrine (4 mg/kg), aqueous and D/M extracts of H. barteri stem bark for 5 days. The in vitro antioxidant property of the aqueous extract was determined by using the reducing power, nitric oxide and total antioxidant capacity assays. Results: The aqueous extract exerted significant (P < 0.05) curative and prophylactic antimalarial activities. The D/M extract exhibited significant curative (P < 0.05) but not prophylactic antiplasmodial effect. The aqueous extract exhibited in vitro antioxidant property with IC50's of (0.930 ± 0.021) mg/mL, (0.800 ± 0.001) mg/mL and (0.22 ± 0.05) mg/mL in the total antioxidant capacity, reducing power and nitric oxide assays. Histological assessment of the liver of aqueous and D/M treated animals did not reveal any sign of toxicity. Conclusions: H. barteri is not toxic which exerted significant curative antiplasmodial effects but the prophylactic property was however fraction dependent. The mechanism of the antiplasmodial activity of H. barteri may partly be mediated by its antioxidant property. |
format |
Article in Journal/Newspaper |
author |
Johnson Nyarko Boampong Akua Afryie Karikari Elvis Ofori Ameyaw |
author_facet |
Johnson Nyarko Boampong Akua Afryie Karikari Elvis Ofori Ameyaw |
author_sort |
Johnson Nyarko Boampong |
title |
In vivo antiplasmodial and in vitro antioxidant properties of stem bark extracts of Haematostaphis barteri |
title_short |
In vivo antiplasmodial and in vitro antioxidant properties of stem bark extracts of Haematostaphis barteri |
title_full |
In vivo antiplasmodial and in vitro antioxidant properties of stem bark extracts of Haematostaphis barteri |
title_fullStr |
In vivo antiplasmodial and in vitro antioxidant properties of stem bark extracts of Haematostaphis barteri |
title_full_unstemmed |
In vivo antiplasmodial and in vitro antioxidant properties of stem bark extracts of Haematostaphis barteri |
title_sort |
in vivo antiplasmodial and in vitro antioxidant properties of stem bark extracts of haematostaphis barteri |
publisher |
Wolters Kluwer Medknow Publications |
publishDate |
2015 |
url |
https://doi.org/10.1016/j.apjtb.2015.02.002 https://doaj.org/article/425c01445c3c4f61863e8f4abfc7ff17 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Asian Pacific Journal of Tropical Biomedicine, Vol 5, Iss 6, Pp 446-450 (2015) |
op_relation |
http://www.sciencedirect.com/science/article/pii/S2221169115000684 https://doaj.org/toc/2221-1691 2221-1691 doi:10.1016/j.apjtb.2015.02.002 https://doaj.org/article/425c01445c3c4f61863e8f4abfc7ff17 |
op_doi |
https://doi.org/10.1016/j.apjtb.2015.02.002 |
container_title |
Asian Pacific Journal of Tropical Biomedicine |
container_volume |
5 |
container_issue |
6 |
container_start_page |
446 |
op_container_end_page |
450 |
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1766344000240877568 |