A study of ticks and tick-borne livestock pathogens in Pakistan.
As obligate blood-feeding arthropods, ticks transmit pathogens to humans and domestic animals more often than other arthropod vectors. Livestock farming plays a vital role in the rural economy of Pakistan, and tick infestation causes serious problems with it. However, research on tick species divers...
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ftdoajarticles:oai:doaj.org/article:4231813a5a0a4dc19431be9e77fd7131 2023-05-15T15:12:27+02:00 A study of ticks and tick-borne livestock pathogens in Pakistan. Shahid Karim Khemraj Budachetri Nabanita Mukherjee Jaclyn Williams Asma Kausar Muhammad Jawadul Hassan Steven Adamson Scot E Dowd Dmitry Apanskevich Abdullah Arijo Zia Uddin Sindhu Muhammad Azam Kakar Raja Muhammad Dilpazir Khan Shafiq Ullah Muhammad Sohail Sajid Abid Ali Zafar Iqbal 2017-06-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0005681 https://doaj.org/article/4231813a5a0a4dc19431be9e77fd7131 EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC5501686?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0005681 https://doaj.org/article/4231813a5a0a4dc19431be9e77fd7131 PLoS Neglected Tropical Diseases, Vol 11, Iss 6, p e0005681 (2017) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2017 ftdoajarticles https://doi.org/10.1371/journal.pntd.0005681 2022-12-31T14:07:43Z As obligate blood-feeding arthropods, ticks transmit pathogens to humans and domestic animals more often than other arthropod vectors. Livestock farming plays a vital role in the rural economy of Pakistan, and tick infestation causes serious problems with it. However, research on tick species diversity and tick-borne pathogens has rarely been conducted in Pakistan. In this study, a systematic investigation of the tick species infesting livestock in different ecological regions of Pakistan was conducted to determine the microbiome and pathobiome diversity in the indigenous ticks.A total of 3,866 tick specimens were morphologically identified as 19 different tick species representing three important hard ticks, Rhipicephalus, Haemaphysalis and Hyalomma, and two soft ticks, Ornithodorus and Argas. The bacterial diversity across these tick species was assessed by bacterial 16S rRNA gene sequencing using a 454-sequencing platform on 10 of the different tick species infesting livestock. The notable genera detected include Ralstonia, Clostridium, Staphylococcus, Rickettsia, Lactococcus, Lactobacillus, Corynebacterium, Enterobacter, and Enterococcus. A survey of Spotted fever group rickettsia from 514 samples from the 13 different tick species generated rickettsial-specific amplicons in 10% (54) of total ticks tested. Only three tick species Rhipicephalus microplus, Hyalomma anatolicum, and H. dromedarii had evidence of infection with "Candidatus Rickettsia amblyommii" a result further verified using a rompB gene-specific quantitative PCR (qPCR) assay. The Hyalomma ticks also tested positive for the piroplasm, Theileria annulata, using a qPCR assay.This study provides information about tick diversity in Pakistan, and pathogenic bacteria in different tick species. Our results showed evidence for Candidatus R. amblyommii infection in Rhipicephalus microplus, H. anatolicum, and H. dromedarii ticks, which also carried T. annulata. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Argas ENVELOPE(126.620,126.620,63.964,63.964) PLOS Neglected Tropical Diseases 11 6 e0005681 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
spellingShingle |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Shahid Karim Khemraj Budachetri Nabanita Mukherjee Jaclyn Williams Asma Kausar Muhammad Jawadul Hassan Steven Adamson Scot E Dowd Dmitry Apanskevich Abdullah Arijo Zia Uddin Sindhu Muhammad Azam Kakar Raja Muhammad Dilpazir Khan Shafiq Ullah Muhammad Sohail Sajid Abid Ali Zafar Iqbal A study of ticks and tick-borne livestock pathogens in Pakistan. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
As obligate blood-feeding arthropods, ticks transmit pathogens to humans and domestic animals more often than other arthropod vectors. Livestock farming plays a vital role in the rural economy of Pakistan, and tick infestation causes serious problems with it. However, research on tick species diversity and tick-borne pathogens has rarely been conducted in Pakistan. In this study, a systematic investigation of the tick species infesting livestock in different ecological regions of Pakistan was conducted to determine the microbiome and pathobiome diversity in the indigenous ticks.A total of 3,866 tick specimens were morphologically identified as 19 different tick species representing three important hard ticks, Rhipicephalus, Haemaphysalis and Hyalomma, and two soft ticks, Ornithodorus and Argas. The bacterial diversity across these tick species was assessed by bacterial 16S rRNA gene sequencing using a 454-sequencing platform on 10 of the different tick species infesting livestock. The notable genera detected include Ralstonia, Clostridium, Staphylococcus, Rickettsia, Lactococcus, Lactobacillus, Corynebacterium, Enterobacter, and Enterococcus. A survey of Spotted fever group rickettsia from 514 samples from the 13 different tick species generated rickettsial-specific amplicons in 10% (54) of total ticks tested. Only three tick species Rhipicephalus microplus, Hyalomma anatolicum, and H. dromedarii had evidence of infection with "Candidatus Rickettsia amblyommii" a result further verified using a rompB gene-specific quantitative PCR (qPCR) assay. The Hyalomma ticks also tested positive for the piroplasm, Theileria annulata, using a qPCR assay.This study provides information about tick diversity in Pakistan, and pathogenic bacteria in different tick species. Our results showed evidence for Candidatus R. amblyommii infection in Rhipicephalus microplus, H. anatolicum, and H. dromedarii ticks, which also carried T. annulata. |
format |
Article in Journal/Newspaper |
author |
Shahid Karim Khemraj Budachetri Nabanita Mukherjee Jaclyn Williams Asma Kausar Muhammad Jawadul Hassan Steven Adamson Scot E Dowd Dmitry Apanskevich Abdullah Arijo Zia Uddin Sindhu Muhammad Azam Kakar Raja Muhammad Dilpazir Khan Shafiq Ullah Muhammad Sohail Sajid Abid Ali Zafar Iqbal |
author_facet |
Shahid Karim Khemraj Budachetri Nabanita Mukherjee Jaclyn Williams Asma Kausar Muhammad Jawadul Hassan Steven Adamson Scot E Dowd Dmitry Apanskevich Abdullah Arijo Zia Uddin Sindhu Muhammad Azam Kakar Raja Muhammad Dilpazir Khan Shafiq Ullah Muhammad Sohail Sajid Abid Ali Zafar Iqbal |
author_sort |
Shahid Karim |
title |
A study of ticks and tick-borne livestock pathogens in Pakistan. |
title_short |
A study of ticks and tick-borne livestock pathogens in Pakistan. |
title_full |
A study of ticks and tick-borne livestock pathogens in Pakistan. |
title_fullStr |
A study of ticks and tick-borne livestock pathogens in Pakistan. |
title_full_unstemmed |
A study of ticks and tick-borne livestock pathogens in Pakistan. |
title_sort |
study of ticks and tick-borne livestock pathogens in pakistan. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2017 |
url |
https://doi.org/10.1371/journal.pntd.0005681 https://doaj.org/article/4231813a5a0a4dc19431be9e77fd7131 |
long_lat |
ENVELOPE(126.620,126.620,63.964,63.964) |
geographic |
Arctic Argas |
geographic_facet |
Arctic Argas |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 11, Iss 6, p e0005681 (2017) |
op_relation |
http://europepmc.org/articles/PMC5501686?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0005681 https://doaj.org/article/4231813a5a0a4dc19431be9e77fd7131 |
op_doi |
https://doi.org/10.1371/journal.pntd.0005681 |
container_title |
PLOS Neglected Tropical Diseases |
container_volume |
11 |
container_issue |
6 |
container_start_page |
e0005681 |
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1766343130650509312 |