Enzyme-treated date plum leave extract ameliorates atopic dermatitis-like skin lesion in hairless mice
Objective: To evaluate the effect of different extracts of Diospyros lotus leaves in atopic dermatitis Methods: Diospyros lotus leaves were extracted in ethanol and treated with or without hydrochloric acid or α-rhamnosidase to obtain three different extracts-ethanol, acid-hydrolyzed, and enzyme-hyd...
Published in: | Asian Pacific Journal of Tropical Biomedicine |
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Wolters Kluwer Medknow Publications
2020
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Online Access: | https://doi.org/10.4103/2221-1691.283937 https://doaj.org/article/422a7a56c75d42d0b81ee36cc825ef9f |
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ftdoajarticles:oai:doaj.org/article:422a7a56c75d42d0b81ee36cc825ef9f 2023-05-15T15:14:42+02:00 Enzyme-treated date plum leave extract ameliorates atopic dermatitis-like skin lesion in hairless mice Byoung Ok Cho Jae Young Shin Ji-Su Kim Denis Nchang Che Hyun Ju Kang Hyeon Jin Kang Hyeonhwa Oh Young-Soo Kim Seon Il Jang 2020-01-01T00:00:00Z https://doi.org/10.4103/2221-1691.283937 https://doaj.org/article/422a7a56c75d42d0b81ee36cc825ef9f EN eng Wolters Kluwer Medknow Publications http://www.apjtb.org/article.asp?issn=2221-1691;year=2020;volume=10;issue=6;spage=239;epage=247;aulast=Cho https://doaj.org/toc/2221-1691 https://doaj.org/toc/2588-9222 2221-1691 2588-9222 doi:10.4103/2221-1691.283937 https://doaj.org/article/422a7a56c75d42d0b81ee36cc825ef9f Asian Pacific Journal of Tropical Biomedicine, Vol 10, Iss 6, Pp 239-247 (2020) date plum leaves enzyme atopic dermatitis inflammation cytokine Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 article 2020 ftdoajarticles https://doi.org/10.4103/2221-1691.283937 2022-12-31T12:41:29Z Objective: To evaluate the effect of different extracts of Diospyros lotus leaves in atopic dermatitis Methods: Diospyros lotus leaves were extracted in ethanol and treated with or without hydrochloric acid or α-rhamnosidase to obtain three different extracts-ethanol, acid-hydrolyzed, and enzyme-hydrolyzed leaf extracts of date plum. The myricitrin content in all samples was measured using HPLC analysis. In vitro antioxidant and anti-inflammatory activities of the extracts were determined by measuring DPPH radical scavenging activities and nitric oxide production in RAW264.7 cells, respectively. Seven- week-old male hairless mice were used to evaluate the anti-atopic dermatitis effects of three extracts in vivo. Splenocytes and mast cells were used to further determine the anti-atopic dermatitis effects of the major compound in the ethanol leaf extract. Results: Enzyme-hydrolyzed leaf extract showed significant in vitro antioxidant and anti-inflammatory activities, and attenuated atopic dermatitis-like skin symptoms and clinical signs more significantly than ethanol and acid-hydrolyzed leaf extracts in 1-fluoro-2,4- dinitrobenzene and house dust mite antigen-treated hairless mice. Enzyme-hydrolyzed leaf extract also suppressed the serum level of immunoglobulin E, interleukin (IL)-4, tumor necrosis factor (TNF)-α, interferon (IFN)-γ, thymic stromal lymphopoietin, and thymus and activation-regulated chemokine in mice with atopic dermatitis. Furthermore, histological analysis revealed that enzyme- hydrolyzed leaf extract suppressed the increased epidermal thickness, dermal infiltration of inflammatory cells, and infiltration and degranulation of mast cells more markedly than the other two extracts in atopic dermatitis-like skin lesions. In addition, this extract effectively inhibited the production of IFN-γ, IL-4,and thymus and activation-regulated chemokine compared with the other two extracts in concanavalin A-stimulated splenocytes. Myricitrin, a major compound of enzyme-hydrolyzed leaf extract, suppressed ... Article in Journal/Newspaper Arctic Mite Directory of Open Access Journals: DOAJ Articles Arctic Asian Pacific Journal of Tropical Biomedicine 10 6 239 |
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topic |
date plum leaves enzyme atopic dermatitis inflammation cytokine Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 |
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date plum leaves enzyme atopic dermatitis inflammation cytokine Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 Byoung Ok Cho Jae Young Shin Ji-Su Kim Denis Nchang Che Hyun Ju Kang Hyeon Jin Kang Hyeonhwa Oh Young-Soo Kim Seon Il Jang Enzyme-treated date plum leave extract ameliorates atopic dermatitis-like skin lesion in hairless mice |
topic_facet |
date plum leaves enzyme atopic dermatitis inflammation cytokine Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 |
description |
Objective: To evaluate the effect of different extracts of Diospyros lotus leaves in atopic dermatitis Methods: Diospyros lotus leaves were extracted in ethanol and treated with or without hydrochloric acid or α-rhamnosidase to obtain three different extracts-ethanol, acid-hydrolyzed, and enzyme-hydrolyzed leaf extracts of date plum. The myricitrin content in all samples was measured using HPLC analysis. In vitro antioxidant and anti-inflammatory activities of the extracts were determined by measuring DPPH radical scavenging activities and nitric oxide production in RAW264.7 cells, respectively. Seven- week-old male hairless mice were used to evaluate the anti-atopic dermatitis effects of three extracts in vivo. Splenocytes and mast cells were used to further determine the anti-atopic dermatitis effects of the major compound in the ethanol leaf extract. Results: Enzyme-hydrolyzed leaf extract showed significant in vitro antioxidant and anti-inflammatory activities, and attenuated atopic dermatitis-like skin symptoms and clinical signs more significantly than ethanol and acid-hydrolyzed leaf extracts in 1-fluoro-2,4- dinitrobenzene and house dust mite antigen-treated hairless mice. Enzyme-hydrolyzed leaf extract also suppressed the serum level of immunoglobulin E, interleukin (IL)-4, tumor necrosis factor (TNF)-α, interferon (IFN)-γ, thymic stromal lymphopoietin, and thymus and activation-regulated chemokine in mice with atopic dermatitis. Furthermore, histological analysis revealed that enzyme- hydrolyzed leaf extract suppressed the increased epidermal thickness, dermal infiltration of inflammatory cells, and infiltration and degranulation of mast cells more markedly than the other two extracts in atopic dermatitis-like skin lesions. In addition, this extract effectively inhibited the production of IFN-γ, IL-4,and thymus and activation-regulated chemokine compared with the other two extracts in concanavalin A-stimulated splenocytes. Myricitrin, a major compound of enzyme-hydrolyzed leaf extract, suppressed ... |
format |
Article in Journal/Newspaper |
author |
Byoung Ok Cho Jae Young Shin Ji-Su Kim Denis Nchang Che Hyun Ju Kang Hyeon Jin Kang Hyeonhwa Oh Young-Soo Kim Seon Il Jang |
author_facet |
Byoung Ok Cho Jae Young Shin Ji-Su Kim Denis Nchang Che Hyun Ju Kang Hyeon Jin Kang Hyeonhwa Oh Young-Soo Kim Seon Il Jang |
author_sort |
Byoung Ok Cho |
title |
Enzyme-treated date plum leave extract ameliorates atopic dermatitis-like skin lesion in hairless mice |
title_short |
Enzyme-treated date plum leave extract ameliorates atopic dermatitis-like skin lesion in hairless mice |
title_full |
Enzyme-treated date plum leave extract ameliorates atopic dermatitis-like skin lesion in hairless mice |
title_fullStr |
Enzyme-treated date plum leave extract ameliorates atopic dermatitis-like skin lesion in hairless mice |
title_full_unstemmed |
Enzyme-treated date plum leave extract ameliorates atopic dermatitis-like skin lesion in hairless mice |
title_sort |
enzyme-treated date plum leave extract ameliorates atopic dermatitis-like skin lesion in hairless mice |
publisher |
Wolters Kluwer Medknow Publications |
publishDate |
2020 |
url |
https://doi.org/10.4103/2221-1691.283937 https://doaj.org/article/422a7a56c75d42d0b81ee36cc825ef9f |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic Mite |
genre_facet |
Arctic Mite |
op_source |
Asian Pacific Journal of Tropical Biomedicine, Vol 10, Iss 6, Pp 239-247 (2020) |
op_relation |
http://www.apjtb.org/article.asp?issn=2221-1691;year=2020;volume=10;issue=6;spage=239;epage=247;aulast=Cho https://doaj.org/toc/2221-1691 https://doaj.org/toc/2588-9222 2221-1691 2588-9222 doi:10.4103/2221-1691.283937 https://doaj.org/article/422a7a56c75d42d0b81ee36cc825ef9f |
op_doi |
https://doi.org/10.4103/2221-1691.283937 |
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Asian Pacific Journal of Tropical Biomedicine |
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239 |
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