Dual real-time PCR method for rapid detection of sole fish and Pangasius bocourti-derived components in fish products
ObjectiveTo establish a dual real-time PCR rapid detection method for sole fish and Pangasius bocourti-derived components.MethodsUniversal TaqMan primers and probe sets were designed according to the mitochondrial 16S rRNA gene sequence of 13 species of sole fish. TaqMan primers and probe sets were...
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ftdoajarticles:oai:doaj.org/article:41fefbada3e04b07941ac117b8c220d3 2023-05-15T15:27:17+02:00 Dual real-time PCR method for rapid detection of sole fish and Pangasius bocourti-derived components in fish products LI Jie QIAN Yunkai WANG Jianchang 2022-05-01T00:00:00Z https://doi.org/10.13590/j.cjfh.2022.03.022 https://doaj.org/article/41fefbada3e04b07941ac117b8c220d3 ZH chi The Editorial Office of Chinese Journal of Food Hygiene http://www.zgspws.com/zgspwszz/article/abstract/202203022?st=article_issue https://doaj.org/toc/1004-8456 1004-8456 doi:10.13590/j.cjfh.2022.03.022 https://doaj.org/article/41fefbada3e04b07941ac117b8c220d3 Zhongguo shipin weisheng zazhi, Vol 34, Iss 3, Pp 539-545 (2022) sole fish pangasius bocourti cynogloddus real-time pcr fish products adulteration detection Food processing and manufacture TP368-456 Nutrition. Foods and food supply TX341-641 article 2022 ftdoajarticles https://doi.org/10.13590/j.cjfh.2022.03.022 2022-12-30T19:26:53Z ObjectiveTo establish a dual real-time PCR rapid detection method for sole fish and Pangasius bocourti-derived components.MethodsUniversal TaqMan primers and probe sets were designed according to the mitochondrial 16S rRNA gene sequence of 13 species of sole fish. TaqMan primers and probe sets were designed according to the mitochondrial cytb gene sequence of Pangasius bocourti, and the amplification reaction system was optimized for real-time PCR to achieve the purpose of rapid detection of products.ResultsThis method had good specificity, and the sensitivity could reach 10-3 ng sole fish DNA. Sole fish 16S rRNA gene could be detected in fish products mixed with carp meal, and the mass fraction sensitivity could reach 1%. The sensitivity could reach 10-4 ng Pangasius bocourti DNA. Pangasius bocourti cytb gene could be detected in fish products mixed with sole fish, atlantic cod meal and rice noodles, the mass fraction sensitivity of sole fish and atlantic cod meal could reach 0.1%, and the mass fraction sensitivity of rice noodles could reach 0.001%.ConclusionThis method had high specificity, high speed and high sensitivity, and could meet the detection requirements of sole fish authenticity and Pangasius bocourti adulteration in fish meat products. Article in Journal/Newspaper atlantic cod Directory of Open Access Journals: DOAJ Articles |
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Directory of Open Access Journals: DOAJ Articles |
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Chinese |
topic |
sole fish pangasius bocourti cynogloddus real-time pcr fish products adulteration detection Food processing and manufacture TP368-456 Nutrition. Foods and food supply TX341-641 |
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sole fish pangasius bocourti cynogloddus real-time pcr fish products adulteration detection Food processing and manufacture TP368-456 Nutrition. Foods and food supply TX341-641 LI Jie QIAN Yunkai WANG Jianchang Dual real-time PCR method for rapid detection of sole fish and Pangasius bocourti-derived components in fish products |
topic_facet |
sole fish pangasius bocourti cynogloddus real-time pcr fish products adulteration detection Food processing and manufacture TP368-456 Nutrition. Foods and food supply TX341-641 |
description |
ObjectiveTo establish a dual real-time PCR rapid detection method for sole fish and Pangasius bocourti-derived components.MethodsUniversal TaqMan primers and probe sets were designed according to the mitochondrial 16S rRNA gene sequence of 13 species of sole fish. TaqMan primers and probe sets were designed according to the mitochondrial cytb gene sequence of Pangasius bocourti, and the amplification reaction system was optimized for real-time PCR to achieve the purpose of rapid detection of products.ResultsThis method had good specificity, and the sensitivity could reach 10-3 ng sole fish DNA. Sole fish 16S rRNA gene could be detected in fish products mixed with carp meal, and the mass fraction sensitivity could reach 1%. The sensitivity could reach 10-4 ng Pangasius bocourti DNA. Pangasius bocourti cytb gene could be detected in fish products mixed with sole fish, atlantic cod meal and rice noodles, the mass fraction sensitivity of sole fish and atlantic cod meal could reach 0.1%, and the mass fraction sensitivity of rice noodles could reach 0.001%.ConclusionThis method had high specificity, high speed and high sensitivity, and could meet the detection requirements of sole fish authenticity and Pangasius bocourti adulteration in fish meat products. |
format |
Article in Journal/Newspaper |
author |
LI Jie QIAN Yunkai WANG Jianchang |
author_facet |
LI Jie QIAN Yunkai WANG Jianchang |
author_sort |
LI Jie |
title |
Dual real-time PCR method for rapid detection of sole fish and Pangasius bocourti-derived components in fish products |
title_short |
Dual real-time PCR method for rapid detection of sole fish and Pangasius bocourti-derived components in fish products |
title_full |
Dual real-time PCR method for rapid detection of sole fish and Pangasius bocourti-derived components in fish products |
title_fullStr |
Dual real-time PCR method for rapid detection of sole fish and Pangasius bocourti-derived components in fish products |
title_full_unstemmed |
Dual real-time PCR method for rapid detection of sole fish and Pangasius bocourti-derived components in fish products |
title_sort |
dual real-time pcr method for rapid detection of sole fish and pangasius bocourti-derived components in fish products |
publisher |
The Editorial Office of Chinese Journal of Food Hygiene |
publishDate |
2022 |
url |
https://doi.org/10.13590/j.cjfh.2022.03.022 https://doaj.org/article/41fefbada3e04b07941ac117b8c220d3 |
genre |
atlantic cod |
genre_facet |
atlantic cod |
op_source |
Zhongguo shipin weisheng zazhi, Vol 34, Iss 3, Pp 539-545 (2022) |
op_relation |
http://www.zgspws.com/zgspwszz/article/abstract/202203022?st=article_issue https://doaj.org/toc/1004-8456 1004-8456 doi:10.13590/j.cjfh.2022.03.022 https://doaj.org/article/41fefbada3e04b07941ac117b8c220d3 |
op_doi |
https://doi.org/10.13590/j.cjfh.2022.03.022 |
_version_ |
1766357726178312192 |