Multilocus sequence typing (MLST) of clinical and environmental isolates of Cryptococcus neoformans and Cryptococcus gattii in six departments of Colombia reveals high genetic diversity
Abstract INTRODUCTION: The average annual incidence of cryptococcosis in Colombia is 0.23 cases per 100,000 inhabitants in the general population, and 1.1 cases per 1000 in inhabitants with Acquired Immune Deficiency Syndrome (AIDS). In addition, the causal fungus has been isolated from the environm...
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ftdoajarticles:oai:doaj.org/article:41c6ef2f91214572892ca58313f55cdd 2023-05-15T15:11:29+02:00 Multilocus sequence typing (MLST) of clinical and environmental isolates of Cryptococcus neoformans and Cryptococcus gattii in six departments of Colombia reveals high genetic diversity Norida Vélez Patricia Escandón 2020-09-01T00:00:00Z https://doi.org/10.1590/0037-8682-0422-2019 https://doaj.org/article/41c6ef2f91214572892ca58313f55cdd EN eng Sociedade Brasileira de Medicina Tropical (SBMT) http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822020000100332&tlng=en https://doaj.org/toc/1678-9849 1678-9849 doi:10.1590/0037-8682-0422-2019 https://doaj.org/article/41c6ef2f91214572892ca58313f55cdd Revista da Sociedade Brasileira de Medicina Tropical, Vol 53 (2020) Cryptococcus neoformans Cryptococcus gattii Multilocus Sequence Typing Incidence Colombia Arctic medicine. Tropical medicine RC955-962 article 2020 ftdoajarticles https://doi.org/10.1590/0037-8682-0422-2019 2022-12-31T03:21:25Z Abstract INTRODUCTION: The average annual incidence of cryptococcosis in Colombia is 0.23 cases per 100,000 inhabitants in the general population, and 1.1 cases per 1000 in inhabitants with Acquired Immune Deficiency Syndrome (AIDS). In addition, the causal fungus has been isolated from the environment, with serotypes A-B and C in different regions. This study aims to determine the genetic association between clinical and environmental isolates of C. neoformans/C. gattii in Colombia. METHODS: Multilocus sequence typing (MLST) was used to identify possible clones, providing information about the epidemiology, ecology, and etiology of this pathogen in Colombia. RESULTS: A total of 110 strains, both clinical (n=61) and environmental (n=49), with 21 MLST sequence types (ST) of C. neoformans (n=14STs) and C. gattii (n=7STs) were identified. The STs which shared clinical and environmental isolate sources were grouped in different geographical categories; for C. neoformans, ST93 was identified in six departments, ST77 in five departments; and for C. gattii, ST25 was identified in three departments and ST79 in two. CONCLUSIONS: High genetic diversity was found in isolates of C. neoformans/gattii by MLST, suggesting the presence of environmental sources harboring strains which may be sources of infection for humans, especially in immunocompromised patients; these data contribute to the information available in the country on the distribution and molecular variability of C. neoformans and C. gattii isolates recovered in Colombia. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Revista da Sociedade Brasileira de Medicina Tropical 53 |
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English |
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Cryptococcus neoformans Cryptococcus gattii Multilocus Sequence Typing Incidence Colombia Arctic medicine. Tropical medicine RC955-962 |
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Cryptococcus neoformans Cryptococcus gattii Multilocus Sequence Typing Incidence Colombia Arctic medicine. Tropical medicine RC955-962 Norida Vélez Patricia Escandón Multilocus sequence typing (MLST) of clinical and environmental isolates of Cryptococcus neoformans and Cryptococcus gattii in six departments of Colombia reveals high genetic diversity |
topic_facet |
Cryptococcus neoformans Cryptococcus gattii Multilocus Sequence Typing Incidence Colombia Arctic medicine. Tropical medicine RC955-962 |
description |
Abstract INTRODUCTION: The average annual incidence of cryptococcosis in Colombia is 0.23 cases per 100,000 inhabitants in the general population, and 1.1 cases per 1000 in inhabitants with Acquired Immune Deficiency Syndrome (AIDS). In addition, the causal fungus has been isolated from the environment, with serotypes A-B and C in different regions. This study aims to determine the genetic association between clinical and environmental isolates of C. neoformans/C. gattii in Colombia. METHODS: Multilocus sequence typing (MLST) was used to identify possible clones, providing information about the epidemiology, ecology, and etiology of this pathogen in Colombia. RESULTS: A total of 110 strains, both clinical (n=61) and environmental (n=49), with 21 MLST sequence types (ST) of C. neoformans (n=14STs) and C. gattii (n=7STs) were identified. The STs which shared clinical and environmental isolate sources were grouped in different geographical categories; for C. neoformans, ST93 was identified in six departments, ST77 in five departments; and for C. gattii, ST25 was identified in three departments and ST79 in two. CONCLUSIONS: High genetic diversity was found in isolates of C. neoformans/gattii by MLST, suggesting the presence of environmental sources harboring strains which may be sources of infection for humans, especially in immunocompromised patients; these data contribute to the information available in the country on the distribution and molecular variability of C. neoformans and C. gattii isolates recovered in Colombia. |
format |
Article in Journal/Newspaper |
author |
Norida Vélez Patricia Escandón |
author_facet |
Norida Vélez Patricia Escandón |
author_sort |
Norida Vélez |
title |
Multilocus sequence typing (MLST) of clinical and environmental isolates of Cryptococcus neoformans and Cryptococcus gattii in six departments of Colombia reveals high genetic diversity |
title_short |
Multilocus sequence typing (MLST) of clinical and environmental isolates of Cryptococcus neoformans and Cryptococcus gattii in six departments of Colombia reveals high genetic diversity |
title_full |
Multilocus sequence typing (MLST) of clinical and environmental isolates of Cryptococcus neoformans and Cryptococcus gattii in six departments of Colombia reveals high genetic diversity |
title_fullStr |
Multilocus sequence typing (MLST) of clinical and environmental isolates of Cryptococcus neoformans and Cryptococcus gattii in six departments of Colombia reveals high genetic diversity |
title_full_unstemmed |
Multilocus sequence typing (MLST) of clinical and environmental isolates of Cryptococcus neoformans and Cryptococcus gattii in six departments of Colombia reveals high genetic diversity |
title_sort |
multilocus sequence typing (mlst) of clinical and environmental isolates of cryptococcus neoformans and cryptococcus gattii in six departments of colombia reveals high genetic diversity |
publisher |
Sociedade Brasileira de Medicina Tropical (SBMT) |
publishDate |
2020 |
url |
https://doi.org/10.1590/0037-8682-0422-2019 https://doaj.org/article/41c6ef2f91214572892ca58313f55cdd |
geographic |
Arctic |
geographic_facet |
Arctic |
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Arctic |
genre_facet |
Arctic |
op_source |
Revista da Sociedade Brasileira de Medicina Tropical, Vol 53 (2020) |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822020000100332&tlng=en https://doaj.org/toc/1678-9849 1678-9849 doi:10.1590/0037-8682-0422-2019 https://doaj.org/article/41c6ef2f91214572892ca58313f55cdd |
op_doi |
https://doi.org/10.1590/0037-8682-0422-2019 |
container_title |
Revista da Sociedade Brasileira de Medicina Tropical |
container_volume |
53 |
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