Resistance of cervical adenocarcinoma cells (HeLa) to venom from the scorpion Centruroides limpidus limpidus
Background : The venom of Centruroides limpidus limpidus (Cll) is a mixture of pharmacologically active principles. The most important of these are toxic proteins that interact both selectively and specifically with different cellular targets such as ion channels. Recently, anticancer properties of...
Published in: | Journal of Venomous Animals and Toxins including Tropical Diseases |
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Online Access: | https://doi.org/10.1186/1678-9199-19-20 https://doaj.org/article/4192c1a957c747b28c3ad26f55b67901 |
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ftdoajarticles:oai:doaj.org/article:4192c1a957c747b28c3ad26f55b67901 2023-05-15T15:10:09+02:00 Resistance of cervical adenocarcinoma cells (HeLa) to venom from the scorpion Centruroides limpidus limpidus José María Eloy Contreras-Ortiz Juan Carlos Vázquez-Chagoyán José Simón Martínez-Castañeda José Guillermo Estrada-Franco José Esteban Aparicio-Burgos Jorge Acosta-Dibarrat Alberto Barbabosa-Pliego 2013-09-01T00:00:00Z https://doi.org/10.1186/1678-9199-19-20 https://doaj.org/article/4192c1a957c747b28c3ad26f55b67901 EN eng SciELO http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992013000100311&lng=en&tlng=en https://doaj.org/toc/1678-9199 1678-9199 doi:10.1186/1678-9199-19-20 https://doaj.org/article/4192c1a957c747b28c3ad26f55b67901 Journal of Venomous Animals and Toxins including Tropical Diseases, Vol 19, Iss 0 (2013) Scorpion venoms Cytotoxic tests Apoptosis HeLa cells Anticancer agents Centruroides toxins Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 article 2013 ftdoajarticles https://doi.org/10.1186/1678-9199-19-20 2022-12-31T03:40:35Z Background : The venom of Centruroides limpidus limpidus (Cll) is a mixture of pharmacologically active principles. The most important of these are toxic proteins that interact both selectively and specifically with different cellular targets such as ion channels. Recently, anticancer properties of the venom from other scorpion species have been described. Studies in vitro have shown that scorpion venom induces cell death, inhibits proliferation and triggers the apoptotic pathway in different cancer cell lines. Herein, after treating human cervical adenocarcinoma (HeLa) cells with Cll crude venom, their cytotoxic activity and apoptosis induction were assessed. Results : Cll crude venom induced cell death in normal macrophages in a dose-dependent manner. However, through viability assays, HeLa cells showed high survival rates after exposure to Cll venom. Also, Cll venom did not induce apoptosis after performing ethidium bromide/acridine orange assays, nor was there any evidence of chromatin condensation or DNA fragmentation. Conclusions : Crude Cll venom exposure was not detrimental to HeLa cell cultures. This may be partially attributable to the absence of specific HeLa cell membrane targets for molecules present in the venom of Centruroides limpidus limpidus. Although these results might discourage additional studies exploring the potential of Cll venom to treat human papilloma cervical cancer, further research is required to explore positive effects of crude Cll venom on other cancer cell lines. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Journal of Venomous Animals and Toxins including Tropical Diseases 19 1 20 |
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Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Scorpion venoms Cytotoxic tests Apoptosis HeLa cells Anticancer agents Centruroides toxins Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 |
spellingShingle |
Scorpion venoms Cytotoxic tests Apoptosis HeLa cells Anticancer agents Centruroides toxins Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 José María Eloy Contreras-Ortiz Juan Carlos Vázquez-Chagoyán José Simón Martínez-Castañeda José Guillermo Estrada-Franco José Esteban Aparicio-Burgos Jorge Acosta-Dibarrat Alberto Barbabosa-Pliego Resistance of cervical adenocarcinoma cells (HeLa) to venom from the scorpion Centruroides limpidus limpidus |
topic_facet |
Scorpion venoms Cytotoxic tests Apoptosis HeLa cells Anticancer agents Centruroides toxins Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 |
description |
Background : The venom of Centruroides limpidus limpidus (Cll) is a mixture of pharmacologically active principles. The most important of these are toxic proteins that interact both selectively and specifically with different cellular targets such as ion channels. Recently, anticancer properties of the venom from other scorpion species have been described. Studies in vitro have shown that scorpion venom induces cell death, inhibits proliferation and triggers the apoptotic pathway in different cancer cell lines. Herein, after treating human cervical adenocarcinoma (HeLa) cells with Cll crude venom, their cytotoxic activity and apoptosis induction were assessed. Results : Cll crude venom induced cell death in normal macrophages in a dose-dependent manner. However, through viability assays, HeLa cells showed high survival rates after exposure to Cll venom. Also, Cll venom did not induce apoptosis after performing ethidium bromide/acridine orange assays, nor was there any evidence of chromatin condensation or DNA fragmentation. Conclusions : Crude Cll venom exposure was not detrimental to HeLa cell cultures. This may be partially attributable to the absence of specific HeLa cell membrane targets for molecules present in the venom of Centruroides limpidus limpidus. Although these results might discourage additional studies exploring the potential of Cll venom to treat human papilloma cervical cancer, further research is required to explore positive effects of crude Cll venom on other cancer cell lines. |
format |
Article in Journal/Newspaper |
author |
José María Eloy Contreras-Ortiz Juan Carlos Vázquez-Chagoyán José Simón Martínez-Castañeda José Guillermo Estrada-Franco José Esteban Aparicio-Burgos Jorge Acosta-Dibarrat Alberto Barbabosa-Pliego |
author_facet |
José María Eloy Contreras-Ortiz Juan Carlos Vázquez-Chagoyán José Simón Martínez-Castañeda José Guillermo Estrada-Franco José Esteban Aparicio-Burgos Jorge Acosta-Dibarrat Alberto Barbabosa-Pliego |
author_sort |
José María Eloy Contreras-Ortiz |
title |
Resistance of cervical adenocarcinoma cells (HeLa) to venom from the scorpion Centruroides limpidus limpidus |
title_short |
Resistance of cervical adenocarcinoma cells (HeLa) to venom from the scorpion Centruroides limpidus limpidus |
title_full |
Resistance of cervical adenocarcinoma cells (HeLa) to venom from the scorpion Centruroides limpidus limpidus |
title_fullStr |
Resistance of cervical adenocarcinoma cells (HeLa) to venom from the scorpion Centruroides limpidus limpidus |
title_full_unstemmed |
Resistance of cervical adenocarcinoma cells (HeLa) to venom from the scorpion Centruroides limpidus limpidus |
title_sort |
resistance of cervical adenocarcinoma cells (hela) to venom from the scorpion centruroides limpidus limpidus |
publisher |
SciELO |
publishDate |
2013 |
url |
https://doi.org/10.1186/1678-9199-19-20 https://doaj.org/article/4192c1a957c747b28c3ad26f55b67901 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Journal of Venomous Animals and Toxins including Tropical Diseases, Vol 19, Iss 0 (2013) |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992013000100311&lng=en&tlng=en https://doaj.org/toc/1678-9199 1678-9199 doi:10.1186/1678-9199-19-20 https://doaj.org/article/4192c1a957c747b28c3ad26f55b67901 |
op_doi |
https://doi.org/10.1186/1678-9199-19-20 |
container_title |
Journal of Venomous Animals and Toxins including Tropical Diseases |
container_volume |
19 |
container_issue |
1 |
container_start_page |
20 |
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1766341201055711232 |