Conservation of A-to-I RNA editing in bowhead whale and pig.
RNA editing is a post-transcriptional process in which nucleotide changes are introduced into an RNA sequence, many of which can contribute to proteomic sequence variation. The most common type of RNA editing, contributing to nearly 99% of all editing events in RNA, is A-to-I (adenosine-to-inosine)...
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ftdoajarticles:oai:doaj.org/article:4185596a8fd244959878d690932f517c 2023-05-15T15:45:59+02:00 Conservation of A-to-I RNA editing in bowhead whale and pig. Knud Larsen Mads Peter Heide-Jørgensen 2021-01-01T00:00:00Z https://doi.org/10.1371/journal.pone.0260081 https://doaj.org/article/4185596a8fd244959878d690932f517c EN eng Public Library of Science (PLoS) https://doi.org/10.1371/journal.pone.0260081 https://doaj.org/toc/1932-6203 1932-6203 doi:10.1371/journal.pone.0260081 https://doaj.org/article/4185596a8fd244959878d690932f517c PLoS ONE, Vol 16, Iss 12, p e0260081 (2021) Medicine R Science Q article 2021 ftdoajarticles https://doi.org/10.1371/journal.pone.0260081 2022-12-31T08:09:45Z RNA editing is a post-transcriptional process in which nucleotide changes are introduced into an RNA sequence, many of which can contribute to proteomic sequence variation. The most common type of RNA editing, contributing to nearly 99% of all editing events in RNA, is A-to-I (adenosine-to-inosine) editing mediated by double-stranded RNA-specific adenosine deaminase (ADAR) enzymes. A-to-I editing at 'recoding' sites results in non-synonymous substitutions in protein-coding sequences. Here, we present studies of the conservation of A-to-I editing in selected mRNAs between pigs, bowhead whales, humans and two shark species. All examined mRNAs-NEIL1, COG3, GRIA2, FLNA, FLNB, IGFBP7, AZIN1, BLCAP, GLI1, SON, HTR2C and ADAR2 -showed conservation of A-to-I editing of recoding sites. In addition, novel editing sites were identified in NEIL1 and GLI1 in bowhead whales. The A-to-I editing site of human NEIL1 in position 242 was conserved in the bowhead and porcine homologues. A novel editing site was discovered in Tyr244. Differential editing was detected at the two adenosines in the NEIL1 242 codon in both pig and bowhead NEIL1 mRNAs in various tissues and organs. No conservation of editing of KCNB1 and EEF1A mRNAs was seen in bowhead whales. In silico analyses revealed conservation of five adenosines in ADAR2, some of which are subject to A-to-I editing in bowheads and pigs, and conservation of a regulatory sequence in GRIA2 mRNA that is responsible for recognition of the ADAR editing enzyme. Article in Journal/Newspaper bowhead whale Directory of Open Access Journals: DOAJ Articles PLOS ONE 16 12 e0260081 |
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Medicine R Science Q Knud Larsen Mads Peter Heide-Jørgensen Conservation of A-to-I RNA editing in bowhead whale and pig. |
topic_facet |
Medicine R Science Q |
description |
RNA editing is a post-transcriptional process in which nucleotide changes are introduced into an RNA sequence, many of which can contribute to proteomic sequence variation. The most common type of RNA editing, contributing to nearly 99% of all editing events in RNA, is A-to-I (adenosine-to-inosine) editing mediated by double-stranded RNA-specific adenosine deaminase (ADAR) enzymes. A-to-I editing at 'recoding' sites results in non-synonymous substitutions in protein-coding sequences. Here, we present studies of the conservation of A-to-I editing in selected mRNAs between pigs, bowhead whales, humans and two shark species. All examined mRNAs-NEIL1, COG3, GRIA2, FLNA, FLNB, IGFBP7, AZIN1, BLCAP, GLI1, SON, HTR2C and ADAR2 -showed conservation of A-to-I editing of recoding sites. In addition, novel editing sites were identified in NEIL1 and GLI1 in bowhead whales. The A-to-I editing site of human NEIL1 in position 242 was conserved in the bowhead and porcine homologues. A novel editing site was discovered in Tyr244. Differential editing was detected at the two adenosines in the NEIL1 242 codon in both pig and bowhead NEIL1 mRNAs in various tissues and organs. No conservation of editing of KCNB1 and EEF1A mRNAs was seen in bowhead whales. In silico analyses revealed conservation of five adenosines in ADAR2, some of which are subject to A-to-I editing in bowheads and pigs, and conservation of a regulatory sequence in GRIA2 mRNA that is responsible for recognition of the ADAR editing enzyme. |
format |
Article in Journal/Newspaper |
author |
Knud Larsen Mads Peter Heide-Jørgensen |
author_facet |
Knud Larsen Mads Peter Heide-Jørgensen |
author_sort |
Knud Larsen |
title |
Conservation of A-to-I RNA editing in bowhead whale and pig. |
title_short |
Conservation of A-to-I RNA editing in bowhead whale and pig. |
title_full |
Conservation of A-to-I RNA editing in bowhead whale and pig. |
title_fullStr |
Conservation of A-to-I RNA editing in bowhead whale and pig. |
title_full_unstemmed |
Conservation of A-to-I RNA editing in bowhead whale and pig. |
title_sort |
conservation of a-to-i rna editing in bowhead whale and pig. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2021 |
url |
https://doi.org/10.1371/journal.pone.0260081 https://doaj.org/article/4185596a8fd244959878d690932f517c |
genre |
bowhead whale |
genre_facet |
bowhead whale |
op_source |
PLoS ONE, Vol 16, Iss 12, p e0260081 (2021) |
op_relation |
https://doi.org/10.1371/journal.pone.0260081 https://doaj.org/toc/1932-6203 1932-6203 doi:10.1371/journal.pone.0260081 https://doaj.org/article/4185596a8fd244959878d690932f517c |
op_doi |
https://doi.org/10.1371/journal.pone.0260081 |
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PLOS ONE |
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16 |
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12 |
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e0260081 |
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