Production of oceanic nitrous oxide by ammonia-oxidizing archaea
The recent finding that microbial ammonia oxidation in the ocean is performed by archaea to a greater extent than by bacteria has drastically changed the view on oceanic nitrification. The numerical dominance of archaeal ammonia-oxidizers (AOA) over their bacterial counterparts (AOB) in large parts...
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ftdoajarticles:oai:doaj.org/article:4175810947b244a5bf502c9dfefe0fd9 2023-05-15T17:36:21+02:00 Production of oceanic nitrous oxide by ammonia-oxidizing archaea C. R. Löscher A. Kock M. Könneke J. LaRoche H. W. Bange R. A. Schmitz 2012-07-01T00:00:00Z https://doi.org/10.5194/bg-9-2419-2012 https://doaj.org/article/4175810947b244a5bf502c9dfefe0fd9 EN eng Copernicus Publications http://www.biogeosciences.net/9/2419/2012/bg-9-2419-2012.pdf https://doaj.org/toc/1726-4170 https://doaj.org/toc/1726-4189 doi:10.5194/bg-9-2419-2012 1726-4170 1726-4189 https://doaj.org/article/4175810947b244a5bf502c9dfefe0fd9 Biogeosciences, Vol 9, Iss 7, Pp 2419-2429 (2012) Ecology QH540-549.5 Life QH501-531 Geology QE1-996.5 article 2012 ftdoajarticles https://doi.org/10.5194/bg-9-2419-2012 2022-12-31T02:51:22Z The recent finding that microbial ammonia oxidation in the ocean is performed by archaea to a greater extent than by bacteria has drastically changed the view on oceanic nitrification. The numerical dominance of archaeal ammonia-oxidizers (AOA) over their bacterial counterparts (AOB) in large parts of the ocean leads to the hypothesis that AOA rather than AOB could be the key organisms for the oceanic production of the strong greenhouse gas nitrous oxide (N 2 O) that occurs as a by-product of nitrification. Very recently, enrichment cultures of marine ammonia-oxidizing archaea have been reported to produce N 2 O. Here, we demonstrate that archaeal ammonia monooxygenase genes ( amoA ) were detectable throughout the water column of the eastern tropical North Atlantic (ETNA) and eastern tropical South Pacific (ETSP) Oceans. Particularly in the ETNA, comparable patterns of abundance and expression of archaeal amoA genes and N 2 O co-occurred in the oxygen minimum, whereas the abundances of bacterial amoA genes were negligible. Moreover, selective inhibition of archaea in seawater incubations from the ETNA decreased the N 2 O production significantly. In studies with the only cultivated marine archaeal ammonia-oxidizer Nitrosopumilus maritimus SCM1, we provide the first direct evidence for N 2 O production in a pure culture of AOA, excluding the involvement of other microorganisms as possibly present in enrichments. N. maritimus showed high N 2 O production rates under low oxygen concentrations comparable to concentrations existing in the oxycline of the ETNA, whereas the N 2 O production from two AOB cultures was comparably low under similar conditions. Based on our findings, we hypothesize that the production of N 2 O in tropical ocean areas results mainly from archaeal nitrification and will be affected by the predicted decrease in dissolved oxygen in the ocean. Article in Journal/Newspaper North Atlantic Directory of Open Access Journals: DOAJ Articles Pacific Biogeosciences 9 7 2419 2429 |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English |
topic |
Ecology QH540-549.5 Life QH501-531 Geology QE1-996.5 |
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Ecology QH540-549.5 Life QH501-531 Geology QE1-996.5 C. R. Löscher A. Kock M. Könneke J. LaRoche H. W. Bange R. A. Schmitz Production of oceanic nitrous oxide by ammonia-oxidizing archaea |
topic_facet |
Ecology QH540-549.5 Life QH501-531 Geology QE1-996.5 |
description |
The recent finding that microbial ammonia oxidation in the ocean is performed by archaea to a greater extent than by bacteria has drastically changed the view on oceanic nitrification. The numerical dominance of archaeal ammonia-oxidizers (AOA) over their bacterial counterparts (AOB) in large parts of the ocean leads to the hypothesis that AOA rather than AOB could be the key organisms for the oceanic production of the strong greenhouse gas nitrous oxide (N 2 O) that occurs as a by-product of nitrification. Very recently, enrichment cultures of marine ammonia-oxidizing archaea have been reported to produce N 2 O. Here, we demonstrate that archaeal ammonia monooxygenase genes ( amoA ) were detectable throughout the water column of the eastern tropical North Atlantic (ETNA) and eastern tropical South Pacific (ETSP) Oceans. Particularly in the ETNA, comparable patterns of abundance and expression of archaeal amoA genes and N 2 O co-occurred in the oxygen minimum, whereas the abundances of bacterial amoA genes were negligible. Moreover, selective inhibition of archaea in seawater incubations from the ETNA decreased the N 2 O production significantly. In studies with the only cultivated marine archaeal ammonia-oxidizer Nitrosopumilus maritimus SCM1, we provide the first direct evidence for N 2 O production in a pure culture of AOA, excluding the involvement of other microorganisms as possibly present in enrichments. N. maritimus showed high N 2 O production rates under low oxygen concentrations comparable to concentrations existing in the oxycline of the ETNA, whereas the N 2 O production from two AOB cultures was comparably low under similar conditions. Based on our findings, we hypothesize that the production of N 2 O in tropical ocean areas results mainly from archaeal nitrification and will be affected by the predicted decrease in dissolved oxygen in the ocean. |
format |
Article in Journal/Newspaper |
author |
C. R. Löscher A. Kock M. Könneke J. LaRoche H. W. Bange R. A. Schmitz |
author_facet |
C. R. Löscher A. Kock M. Könneke J. LaRoche H. W. Bange R. A. Schmitz |
author_sort |
C. R. Löscher |
title |
Production of oceanic nitrous oxide by ammonia-oxidizing archaea |
title_short |
Production of oceanic nitrous oxide by ammonia-oxidizing archaea |
title_full |
Production of oceanic nitrous oxide by ammonia-oxidizing archaea |
title_fullStr |
Production of oceanic nitrous oxide by ammonia-oxidizing archaea |
title_full_unstemmed |
Production of oceanic nitrous oxide by ammonia-oxidizing archaea |
title_sort |
production of oceanic nitrous oxide by ammonia-oxidizing archaea |
publisher |
Copernicus Publications |
publishDate |
2012 |
url |
https://doi.org/10.5194/bg-9-2419-2012 https://doaj.org/article/4175810947b244a5bf502c9dfefe0fd9 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
North Atlantic |
genre_facet |
North Atlantic |
op_source |
Biogeosciences, Vol 9, Iss 7, Pp 2419-2429 (2012) |
op_relation |
http://www.biogeosciences.net/9/2419/2012/bg-9-2419-2012.pdf https://doaj.org/toc/1726-4170 https://doaj.org/toc/1726-4189 doi:10.5194/bg-9-2419-2012 1726-4170 1726-4189 https://doaj.org/article/4175810947b244a5bf502c9dfefe0fd9 |
op_doi |
https://doi.org/10.5194/bg-9-2419-2012 |
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Biogeosciences |
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9 |
container_issue |
7 |
container_start_page |
2419 |
op_container_end_page |
2429 |
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1766135798909894656 |