Serological responses and biomarker evaluation in mice and pigs exposed to tsetse fly bites.
BACKGROUND: Tsetse flies are obligate blood-feeding insects that transmit African trypanosomes responsible for human sleeping sickness and nagana in livestock. The tsetse salivary proteome contains a highly immunogenic family of the endonuclease-like Tsal proteins. In this study, a recombinant versi...
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ftdoajarticles:oai:doaj.org/article:4153d1a8708044fb8e74c416d98f39db 2023-05-15T15:16:36+02:00 Serological responses and biomarker evaluation in mice and pigs exposed to tsetse fly bites. Guy Caljon Reta Duguma Reginald De Deken Stijn Schauvliege Frank Gasthuys Luc Duchateau Jan Van Den Abbeele 2014-05-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0002911 https://doaj.org/article/4153d1a8708044fb8e74c416d98f39db EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC4031185?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0002911 https://doaj.org/article/4153d1a8708044fb8e74c416d98f39db PLoS Neglected Tropical Diseases, Vol 8, Iss 5, p e2911 (2014) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2014 ftdoajarticles https://doi.org/10.1371/journal.pntd.0002911 2022-12-31T12:24:11Z BACKGROUND: Tsetse flies are obligate blood-feeding insects that transmit African trypanosomes responsible for human sleeping sickness and nagana in livestock. The tsetse salivary proteome contains a highly immunogenic family of the endonuclease-like Tsal proteins. In this study, a recombinant version of Tsal1 (rTsal1) was evaluated in an indirect ELISA to quantify the contact with total Glossina morsitans morsitans saliva, and thus the tsetse fly bite exposure. METHODOLOGY/PRINCIPAL FINDINGS: Mice and pigs were experimentally exposed to different G. m. morsitans exposure regimens, followed by a long-term follow-up of the specific antibody responses against total tsetse fly saliva and rTsal1. In mice, a single tsetse fly bite was sufficient to induce detectable IgG antibody responses with an estimated half-life of 36-40 days. Specific antibody responses could be detected for more than a year after initial exposure, and a single bite was sufficient to boost anti-saliva immunity. Also, plasmas collected from tsetse-exposed pigs displayed increased anti-rTsal1 and anti-saliva IgG levels that correlated with the exposure intensity. A strong correlation between the detection of anti-rTsal1 and anti-saliva responses was recorded. The ELISA test performance and intra-laboratory repeatability was adequate in the two tested animal models. Cross-reactivity of the mouse IgGs induced by exposure to different Glossina species (G. m. morsitans, G. pallidipes, G. palpalis gambiensis and G. fuscipes) and other hematophagous insects (Stomoxys calcitrans and Tabanus yao) was evaluated. CONCLUSION: This study illustrates the potential use of rTsal1 from G. m. morsitans as a sensitive biomarker of exposure to a broad range of Glossina species. We propose that the detection of anti-rTsal1 IgGs could be a promising serological indicator of tsetse fly presence that will be a valuable tool to monitor the impact of tsetse control efforts on the African continent. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLoS Neglected Tropical Diseases 8 5 e2911 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
spellingShingle |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Guy Caljon Reta Duguma Reginald De Deken Stijn Schauvliege Frank Gasthuys Luc Duchateau Jan Van Den Abbeele Serological responses and biomarker evaluation in mice and pigs exposed to tsetse fly bites. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
BACKGROUND: Tsetse flies are obligate blood-feeding insects that transmit African trypanosomes responsible for human sleeping sickness and nagana in livestock. The tsetse salivary proteome contains a highly immunogenic family of the endonuclease-like Tsal proteins. In this study, a recombinant version of Tsal1 (rTsal1) was evaluated in an indirect ELISA to quantify the contact with total Glossina morsitans morsitans saliva, and thus the tsetse fly bite exposure. METHODOLOGY/PRINCIPAL FINDINGS: Mice and pigs were experimentally exposed to different G. m. morsitans exposure regimens, followed by a long-term follow-up of the specific antibody responses against total tsetse fly saliva and rTsal1. In mice, a single tsetse fly bite was sufficient to induce detectable IgG antibody responses with an estimated half-life of 36-40 days. Specific antibody responses could be detected for more than a year after initial exposure, and a single bite was sufficient to boost anti-saliva immunity. Also, plasmas collected from tsetse-exposed pigs displayed increased anti-rTsal1 and anti-saliva IgG levels that correlated with the exposure intensity. A strong correlation between the detection of anti-rTsal1 and anti-saliva responses was recorded. The ELISA test performance and intra-laboratory repeatability was adequate in the two tested animal models. Cross-reactivity of the mouse IgGs induced by exposure to different Glossina species (G. m. morsitans, G. pallidipes, G. palpalis gambiensis and G. fuscipes) and other hematophagous insects (Stomoxys calcitrans and Tabanus yao) was evaluated. CONCLUSION: This study illustrates the potential use of rTsal1 from G. m. morsitans as a sensitive biomarker of exposure to a broad range of Glossina species. We propose that the detection of anti-rTsal1 IgGs could be a promising serological indicator of tsetse fly presence that will be a valuable tool to monitor the impact of tsetse control efforts on the African continent. |
format |
Article in Journal/Newspaper |
author |
Guy Caljon Reta Duguma Reginald De Deken Stijn Schauvliege Frank Gasthuys Luc Duchateau Jan Van Den Abbeele |
author_facet |
Guy Caljon Reta Duguma Reginald De Deken Stijn Schauvliege Frank Gasthuys Luc Duchateau Jan Van Den Abbeele |
author_sort |
Guy Caljon |
title |
Serological responses and biomarker evaluation in mice and pigs exposed to tsetse fly bites. |
title_short |
Serological responses and biomarker evaluation in mice and pigs exposed to tsetse fly bites. |
title_full |
Serological responses and biomarker evaluation in mice and pigs exposed to tsetse fly bites. |
title_fullStr |
Serological responses and biomarker evaluation in mice and pigs exposed to tsetse fly bites. |
title_full_unstemmed |
Serological responses and biomarker evaluation in mice and pigs exposed to tsetse fly bites. |
title_sort |
serological responses and biomarker evaluation in mice and pigs exposed to tsetse fly bites. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2014 |
url |
https://doi.org/10.1371/journal.pntd.0002911 https://doaj.org/article/4153d1a8708044fb8e74c416d98f39db |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 8, Iss 5, p e2911 (2014) |
op_relation |
http://europepmc.org/articles/PMC4031185?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0002911 https://doaj.org/article/4153d1a8708044fb8e74c416d98f39db |
op_doi |
https://doi.org/10.1371/journal.pntd.0002911 |
container_title |
PLoS Neglected Tropical Diseases |
container_volume |
8 |
container_issue |
5 |
container_start_page |
e2911 |
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1766346893814661120 |