Status of potential Pf ATP6 molecular markers for artemisinin resistance in Suriname
Abstract Background Polymorphisms within the PfATP6 gene have been indicated as potential molecular markers for artemisinin efficacy. Since 2004, the use of artemisinin combination therapy (ACT) was introduced as first-line treatment of the uncomplicated malaria cases in Suriname. The aim of this re...
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ftdoajarticles:oai:doaj.org/article:40828c9f17d845ed97a304b6dae70de2 2023-05-15T15:10:34+02:00 Status of potential Pf ATP6 molecular markers for artemisinin resistance in Suriname Adhin Malti R Labadie-Bracho Mergiory Vreden Stephen G 2012-09-01T00:00:00Z https://doi.org/10.1186/1475-2875-11-322 https://doaj.org/article/40828c9f17d845ed97a304b6dae70de2 EN eng BMC http://www.malariajournal.com/content/11/1/322 https://doaj.org/toc/1475-2875 doi:10.1186/1475-2875-11-322 1475-2875 https://doaj.org/article/40828c9f17d845ed97a304b6dae70de2 Malaria Journal, Vol 11, Iss 1, p 322 (2012) Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 2012 ftdoajarticles https://doi.org/10.1186/1475-2875-11-322 2022-12-31T12:56:09Z Abstract Background Polymorphisms within the PfATP6 gene have been indicated as potential molecular markers for artemisinin efficacy. Since 2004, the use of artemisinin combination therapy (ACT) was introduced as first-line treatment of the uncomplicated malaria cases in Suriname. The aim of this research was to determine changes in Suriname in the status of the polymorphic markers in the PfATP6 gene before and after the adoption of the ACT-regimen, particularly of the S769N mutation, which was reported to be associated with in vitro Artemether resistance in the neighboring country French Guiana. Methods The PfATP6 gene from Plasmodium falciparum parasites in Suriname was investigated in 28 samples using PCR amplification and restriction enzyme analysis, to assess and determine the prevalence of potentially interesting single nucleotide polymorphisms. The polymorphisms [L263E; A623E; S769N], which may be associated with the artemisinin resistant phenotype were characterized in parasites from three endemic regions before and after the adoption of the ACT-regimen. In addition, the status of these molecular markers was compared in paired P. falciparum isolates from patients with recurring malaria after controlled ACT. Results All the investigated samples exhibit the wild-type genotype at all three positions; L263, A623, S769. Conclusion All investigated isolates before and after the adoption of the ACT-regimen and independent of endemic region harbored the wild-type genotype for the three investigated polymorphisms. The study revealed that decreased artemisinin susceptibility could occur independent from PfATP6 mutations, challenging the assumption that artemisinin resistance is associated with these mutations in the PfATP6 gene. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Malaria Journal 11 1 |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
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English |
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Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
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Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 Adhin Malti R Labadie-Bracho Mergiory Vreden Stephen G Status of potential Pf ATP6 molecular markers for artemisinin resistance in Suriname |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
Abstract Background Polymorphisms within the PfATP6 gene have been indicated as potential molecular markers for artemisinin efficacy. Since 2004, the use of artemisinin combination therapy (ACT) was introduced as first-line treatment of the uncomplicated malaria cases in Suriname. The aim of this research was to determine changes in Suriname in the status of the polymorphic markers in the PfATP6 gene before and after the adoption of the ACT-regimen, particularly of the S769N mutation, which was reported to be associated with in vitro Artemether resistance in the neighboring country French Guiana. Methods The PfATP6 gene from Plasmodium falciparum parasites in Suriname was investigated in 28 samples using PCR amplification and restriction enzyme analysis, to assess and determine the prevalence of potentially interesting single nucleotide polymorphisms. The polymorphisms [L263E; A623E; S769N], which may be associated with the artemisinin resistant phenotype were characterized in parasites from three endemic regions before and after the adoption of the ACT-regimen. In addition, the status of these molecular markers was compared in paired P. falciparum isolates from patients with recurring malaria after controlled ACT. Results All the investigated samples exhibit the wild-type genotype at all three positions; L263, A623, S769. Conclusion All investigated isolates before and after the adoption of the ACT-regimen and independent of endemic region harbored the wild-type genotype for the three investigated polymorphisms. The study revealed that decreased artemisinin susceptibility could occur independent from PfATP6 mutations, challenging the assumption that artemisinin resistance is associated with these mutations in the PfATP6 gene. |
format |
Article in Journal/Newspaper |
author |
Adhin Malti R Labadie-Bracho Mergiory Vreden Stephen G |
author_facet |
Adhin Malti R Labadie-Bracho Mergiory Vreden Stephen G |
author_sort |
Adhin Malti R |
title |
Status of potential Pf ATP6 molecular markers for artemisinin resistance in Suriname |
title_short |
Status of potential Pf ATP6 molecular markers for artemisinin resistance in Suriname |
title_full |
Status of potential Pf ATP6 molecular markers for artemisinin resistance in Suriname |
title_fullStr |
Status of potential Pf ATP6 molecular markers for artemisinin resistance in Suriname |
title_full_unstemmed |
Status of potential Pf ATP6 molecular markers for artemisinin resistance in Suriname |
title_sort |
status of potential pf atp6 molecular markers for artemisinin resistance in suriname |
publisher |
BMC |
publishDate |
2012 |
url |
https://doi.org/10.1186/1475-2875-11-322 https://doaj.org/article/40828c9f17d845ed97a304b6dae70de2 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Malaria Journal, Vol 11, Iss 1, p 322 (2012) |
op_relation |
http://www.malariajournal.com/content/11/1/322 https://doaj.org/toc/1475-2875 doi:10.1186/1475-2875-11-322 1475-2875 https://doaj.org/article/40828c9f17d845ed97a304b6dae70de2 |
op_doi |
https://doi.org/10.1186/1475-2875-11-322 |
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Malaria Journal |
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11 |
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