Genetic diversity of Leishmania donovani isolates from cutaneous lesions of military personnel in the Mullaitivu and Kilinochchi districts of the Northern Province, Sri Lanka
Objective: To compare the DNA sequences of Leishmania (L.)donovani isolated from individuals in two districts of the Northern Province with other parts of Sri Lanka and neighboring countries. Methods: Samples were collected from military personnel at the Army Hospital, Narahenpita, Sri Lanka from No...
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ftdoajarticles:oai:doaj.org/article:406ba8907c0849feba8d13cdaadd04f0 2023-05-15T15:10:50+02:00 Genetic diversity of Leishmania donovani isolates from cutaneous lesions of military personnel in the Mullaitivu and Kilinochchi districts of the Northern Province, Sri Lanka Tharaka Wijerathna Nayana Gunathilaka Saveen Semege Nishantha Pathirana Wasana Rodrigo Deepika Fernando 2022-01-01T00:00:00Z https://doi.org/10.4103/1995-7645.354420 https://doaj.org/article/406ba8907c0849feba8d13cdaadd04f0 EN eng Wolters Kluwer Medknow Publications http://www.apjtm.org/article.asp?issn=1995-7645;year=2022;volume=15;issue=9;spage=418;epage=424;aulast=Wijerathna https://doaj.org/toc/2352-4146 2352-4146 doi:10.4103/1995-7645.354420 https://doaj.org/article/406ba8907c0849feba8d13cdaadd04f0 Asian Pacific Journal of Tropical Medicine, Vol 15, Iss 9, Pp 418-424 (2022) leishmaniasis sri lanka phylogenetics kilinochchi mullaitivu northern province Arctic medicine. Tropical medicine RC955-962 article 2022 ftdoajarticles https://doi.org/10.4103/1995-7645.354420 2022-12-30T23:30:08Z Objective: To compare the DNA sequences of Leishmania (L.)donovani isolated from individuals in two districts of the Northern Province with other parts of Sri Lanka and neighboring countries. Methods: Samples were collected from military personnel at the Army Hospital, Narahenpita, Sri Lanka from November 2018 to March 2020. A portion of the samples was fixed, stained with Giemsa and observed under the light microscope. The genomic The DNA was extracted from the remaining portion of the samples using DNEasy blood tissue kit (Qiagen, Germany) and amplified using Leishmania genus-specific primers for molecular diagnosis initially. DNA was amplified using L. donovani species-specific primers by PCR and the amplified product was sequenced for comparison of nucleotide sequences. Results: Out of 76 suspected patients, at least one biological sample of 45 (59.2%) was positive for L. amastigotes upon microscopy. Overall, 33 (43.4%) were positive in Leishmania genus-specific PCR, but only 23 (30.3%) were positive in L. donovani specific PCR. The dendrogram indicates that the current sequences clustered together with those from Nepal and Gampaha districts (Western Province), Sri Lanka, while the Indian and Eastern African sequences clustered separately. Conclusions: The genetic diversity was low among the isolates, indicating a single and possibly a local point of origin. However, the similarity of Sri Lankan and Nepal strains indicate a possibility of a shared point of origin, which needs more extensive evidence to confirm. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Indian Asian Pacific Journal of Tropical Medicine 15 9 418 |
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Directory of Open Access Journals: DOAJ Articles |
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English |
topic |
leishmaniasis sri lanka phylogenetics kilinochchi mullaitivu northern province Arctic medicine. Tropical medicine RC955-962 |
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leishmaniasis sri lanka phylogenetics kilinochchi mullaitivu northern province Arctic medicine. Tropical medicine RC955-962 Tharaka Wijerathna Nayana Gunathilaka Saveen Semege Nishantha Pathirana Wasana Rodrigo Deepika Fernando Genetic diversity of Leishmania donovani isolates from cutaneous lesions of military personnel in the Mullaitivu and Kilinochchi districts of the Northern Province, Sri Lanka |
topic_facet |
leishmaniasis sri lanka phylogenetics kilinochchi mullaitivu northern province Arctic medicine. Tropical medicine RC955-962 |
description |
Objective: To compare the DNA sequences of Leishmania (L.)donovani isolated from individuals in two districts of the Northern Province with other parts of Sri Lanka and neighboring countries. Methods: Samples were collected from military personnel at the Army Hospital, Narahenpita, Sri Lanka from November 2018 to March 2020. A portion of the samples was fixed, stained with Giemsa and observed under the light microscope. The genomic The DNA was extracted from the remaining portion of the samples using DNEasy blood tissue kit (Qiagen, Germany) and amplified using Leishmania genus-specific primers for molecular diagnosis initially. DNA was amplified using L. donovani species-specific primers by PCR and the amplified product was sequenced for comparison of nucleotide sequences. Results: Out of 76 suspected patients, at least one biological sample of 45 (59.2%) was positive for L. amastigotes upon microscopy. Overall, 33 (43.4%) were positive in Leishmania genus-specific PCR, but only 23 (30.3%) were positive in L. donovani specific PCR. The dendrogram indicates that the current sequences clustered together with those from Nepal and Gampaha districts (Western Province), Sri Lanka, while the Indian and Eastern African sequences clustered separately. Conclusions: The genetic diversity was low among the isolates, indicating a single and possibly a local point of origin. However, the similarity of Sri Lankan and Nepal strains indicate a possibility of a shared point of origin, which needs more extensive evidence to confirm. |
format |
Article in Journal/Newspaper |
author |
Tharaka Wijerathna Nayana Gunathilaka Saveen Semege Nishantha Pathirana Wasana Rodrigo Deepika Fernando |
author_facet |
Tharaka Wijerathna Nayana Gunathilaka Saveen Semege Nishantha Pathirana Wasana Rodrigo Deepika Fernando |
author_sort |
Tharaka Wijerathna |
title |
Genetic diversity of Leishmania donovani isolates from cutaneous lesions of military personnel in the Mullaitivu and Kilinochchi districts of the Northern Province, Sri Lanka |
title_short |
Genetic diversity of Leishmania donovani isolates from cutaneous lesions of military personnel in the Mullaitivu and Kilinochchi districts of the Northern Province, Sri Lanka |
title_full |
Genetic diversity of Leishmania donovani isolates from cutaneous lesions of military personnel in the Mullaitivu and Kilinochchi districts of the Northern Province, Sri Lanka |
title_fullStr |
Genetic diversity of Leishmania donovani isolates from cutaneous lesions of military personnel in the Mullaitivu and Kilinochchi districts of the Northern Province, Sri Lanka |
title_full_unstemmed |
Genetic diversity of Leishmania donovani isolates from cutaneous lesions of military personnel in the Mullaitivu and Kilinochchi districts of the Northern Province, Sri Lanka |
title_sort |
genetic diversity of leishmania donovani isolates from cutaneous lesions of military personnel in the mullaitivu and kilinochchi districts of the northern province, sri lanka |
publisher |
Wolters Kluwer Medknow Publications |
publishDate |
2022 |
url |
https://doi.org/10.4103/1995-7645.354420 https://doaj.org/article/406ba8907c0849feba8d13cdaadd04f0 |
geographic |
Arctic Indian |
geographic_facet |
Arctic Indian |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Asian Pacific Journal of Tropical Medicine, Vol 15, Iss 9, Pp 418-424 (2022) |
op_relation |
http://www.apjtm.org/article.asp?issn=1995-7645;year=2022;volume=15;issue=9;spage=418;epage=424;aulast=Wijerathna https://doaj.org/toc/2352-4146 2352-4146 doi:10.4103/1995-7645.354420 https://doaj.org/article/406ba8907c0849feba8d13cdaadd04f0 |
op_doi |
https://doi.org/10.4103/1995-7645.354420 |
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Asian Pacific Journal of Tropical Medicine |
container_volume |
15 |
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9 |
container_start_page |
418 |
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