Steroid implants for the induction of vitellogenesis in feminized European silver eels (Anguilla anguilla L.)
Assisted propagation of the European eel will lead to a closed production cycle supplying the aquaculture industry with juvenile glass eels. Females require long-term weekly treatment with pituitary extract (PE), which is stressful and causes abnormalities in oogenesis. We tested the effects of 17α-...
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Frontiers Media S.A.
2022
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Online Access: | https://doi.org/10.3389/fgene.2022.969202 https://doaj.org/article/40663605c22f4d7196b8d2cbf42347f8 |
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ftdoajarticles:oai:doaj.org/article:40663605c22f4d7196b8d2cbf42347f8 2023-05-15T13:28:18+02:00 Steroid implants for the induction of vitellogenesis in feminized European silver eels (Anguilla anguilla L.) Arjan P. Palstra Lotte J. Bouwman Pauline Jéhannet Leo Kruijt Henk Schipper Marco H. Blokland William Swinkels Leon T. N. Heinsbroek P. Mark Lokman 2022-08-01T00:00:00Z https://doi.org/10.3389/fgene.2022.969202 https://doaj.org/article/40663605c22f4d7196b8d2cbf42347f8 EN eng Frontiers Media S.A. https://www.frontiersin.org/articles/10.3389/fgene.2022.969202/full https://doaj.org/toc/1664-8021 1664-8021 doi:10.3389/fgene.2022.969202 https://doaj.org/article/40663605c22f4d7196b8d2cbf42347f8 Frontiers in Genetics, Vol 13 (2022) aquaculture eel reproduction 11-ketotestosterone (11KT) FSH receptor steroidogenesis liquid chromatography mass spectrometry (LCMS) Genetics QH426-470 article 2022 ftdoajarticles https://doi.org/10.3389/fgene.2022.969202 2022-12-30T20:41:51Z Assisted propagation of the European eel will lead to a closed production cycle supplying the aquaculture industry with juvenile glass eels. Females require long-term weekly treatment with pituitary extract (PE), which is stressful and causes abnormalities in oogenesis. We tested the effects of 17α-methyltestosterone (17 MT), as potent androgen activating the androgen receptor, and 17β-estradiol (E2), as an inducer of vitellogenesis, to shorten the duration of PE treatment.Four groups of feminized eels were subjected to a simulated migration and subsequent injection with implants containing 17 MT (17 MT-group), E2 (E2-group) or 17 MT plus E2 (17 MT + E2-group) to test for synergistic effects, or without any steroids as controls (C-group). The effects of a 2-months treatment were investigated by determining the eye index (EI), hepatosomatic and gonadosomatic index (HSI and GSI, respectively), plasma steroid concentrations by liquid chromatography mass spectrometry (LCMS), gonadal histology, expression of androgen receptors a and b (ara, arb); estrogen receptor 1 (esr1); FSH receptor (fshr); vitellogenin receptor (vtgr) and aromatase (cyp19), and the required number of weekly PE injections to fully mature. For many parameters, both the 17 MT and E2 groups showed an increase vs. controls, with the 17 MT + E2 group showing a synergistic effect, as seen for EI, GSI (3.4 for 17 MT and for E2, 6.6 for 17 MT + E2), oocyte diameter and ara, arb and esr1 expression. Concentrations of almost all focal steroids decreased with simulated migration and steroid treatment. Only eels of the 17 MT-group showed increased expression of cyp19 and of fshr, while fshr expression increased 44-fold in the 17 MT + E2 group, highlighting that co-implantation is most effective in raising fshr mRNA levels. Specific for eels of the E2 groups were vitellogenesis-associated changes such as an increase of HSI, plasma E2, and presence of yolk in the oocytes. Steroid treatments reduced the duration of PE treatment, again synergistically for ... Article in Journal/Newspaper Anguilla anguilla Directory of Open Access Journals: DOAJ Articles Frontiers in Genetics 13 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
aquaculture eel reproduction 11-ketotestosterone (11KT) FSH receptor steroidogenesis liquid chromatography mass spectrometry (LCMS) Genetics QH426-470 |
spellingShingle |
aquaculture eel reproduction 11-ketotestosterone (11KT) FSH receptor steroidogenesis liquid chromatography mass spectrometry (LCMS) Genetics QH426-470 Arjan P. Palstra Lotte J. Bouwman Pauline Jéhannet Leo Kruijt Henk Schipper Marco H. Blokland William Swinkels Leon T. N. Heinsbroek P. Mark Lokman Steroid implants for the induction of vitellogenesis in feminized European silver eels (Anguilla anguilla L.) |
topic_facet |
aquaculture eel reproduction 11-ketotestosterone (11KT) FSH receptor steroidogenesis liquid chromatography mass spectrometry (LCMS) Genetics QH426-470 |
description |
Assisted propagation of the European eel will lead to a closed production cycle supplying the aquaculture industry with juvenile glass eels. Females require long-term weekly treatment with pituitary extract (PE), which is stressful and causes abnormalities in oogenesis. We tested the effects of 17α-methyltestosterone (17 MT), as potent androgen activating the androgen receptor, and 17β-estradiol (E2), as an inducer of vitellogenesis, to shorten the duration of PE treatment.Four groups of feminized eels were subjected to a simulated migration and subsequent injection with implants containing 17 MT (17 MT-group), E2 (E2-group) or 17 MT plus E2 (17 MT + E2-group) to test for synergistic effects, or without any steroids as controls (C-group). The effects of a 2-months treatment were investigated by determining the eye index (EI), hepatosomatic and gonadosomatic index (HSI and GSI, respectively), plasma steroid concentrations by liquid chromatography mass spectrometry (LCMS), gonadal histology, expression of androgen receptors a and b (ara, arb); estrogen receptor 1 (esr1); FSH receptor (fshr); vitellogenin receptor (vtgr) and aromatase (cyp19), and the required number of weekly PE injections to fully mature. For many parameters, both the 17 MT and E2 groups showed an increase vs. controls, with the 17 MT + E2 group showing a synergistic effect, as seen for EI, GSI (3.4 for 17 MT and for E2, 6.6 for 17 MT + E2), oocyte diameter and ara, arb and esr1 expression. Concentrations of almost all focal steroids decreased with simulated migration and steroid treatment. Only eels of the 17 MT-group showed increased expression of cyp19 and of fshr, while fshr expression increased 44-fold in the 17 MT + E2 group, highlighting that co-implantation is most effective in raising fshr mRNA levels. Specific for eels of the E2 groups were vitellogenesis-associated changes such as an increase of HSI, plasma E2, and presence of yolk in the oocytes. Steroid treatments reduced the duration of PE treatment, again synergistically for ... |
format |
Article in Journal/Newspaper |
author |
Arjan P. Palstra Lotte J. Bouwman Pauline Jéhannet Leo Kruijt Henk Schipper Marco H. Blokland William Swinkels Leon T. N. Heinsbroek P. Mark Lokman |
author_facet |
Arjan P. Palstra Lotte J. Bouwman Pauline Jéhannet Leo Kruijt Henk Schipper Marco H. Blokland William Swinkels Leon T. N. Heinsbroek P. Mark Lokman |
author_sort |
Arjan P. Palstra |
title |
Steroid implants for the induction of vitellogenesis in feminized European silver eels (Anguilla anguilla L.) |
title_short |
Steroid implants for the induction of vitellogenesis in feminized European silver eels (Anguilla anguilla L.) |
title_full |
Steroid implants for the induction of vitellogenesis in feminized European silver eels (Anguilla anguilla L.) |
title_fullStr |
Steroid implants for the induction of vitellogenesis in feminized European silver eels (Anguilla anguilla L.) |
title_full_unstemmed |
Steroid implants for the induction of vitellogenesis in feminized European silver eels (Anguilla anguilla L.) |
title_sort |
steroid implants for the induction of vitellogenesis in feminized european silver eels (anguilla anguilla l.) |
publisher |
Frontiers Media S.A. |
publishDate |
2022 |
url |
https://doi.org/10.3389/fgene.2022.969202 https://doaj.org/article/40663605c22f4d7196b8d2cbf42347f8 |
genre |
Anguilla anguilla |
genre_facet |
Anguilla anguilla |
op_source |
Frontiers in Genetics, Vol 13 (2022) |
op_relation |
https://www.frontiersin.org/articles/10.3389/fgene.2022.969202/full https://doaj.org/toc/1664-8021 1664-8021 doi:10.3389/fgene.2022.969202 https://doaj.org/article/40663605c22f4d7196b8d2cbf42347f8 |
op_doi |
https://doi.org/10.3389/fgene.2022.969202 |
container_title |
Frontiers in Genetics |
container_volume |
13 |
_version_ |
1766403340567052288 |