A newly validated high-performance liquid chromatography method with diode array ultraviolet detection for analysis of the antimalarial drug primaquine in the blood plasma
Abstract INTRODUCTION: Primaquine (PQ) diphosphate is an 8-aminoquinoline antimalarial drug with unique therapeutic properties. It is the only drug that prevents relapses of Plasmodium vivax or Plasmodium ovale infections. In this study, a fast, sensitive, cost-effective, and robust method for the e...
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ftdoajarticles:oai:doaj.org/article:4047c81bc4c94769a8b9d723ac395e81 2023-05-15T15:13:07+02:00 A newly validated high-performance liquid chromatography method with diode array ultraviolet detection for analysis of the antimalarial drug primaquine in the blood plasma Ana Paula Barbosa do Carmo Manoella Borborema Stephan Ribeiro Ana Cecilia Xavier De-Oliveira Francisco Jose Roma Paumgartten Davyson de Lima Moreira https://doi.org/10.1590/0037-8682-0023-2017 https://doaj.org/article/4047c81bc4c94769a8b9d723ac395e81 EN eng Sociedade Brasileira de Medicina Tropical (SBMT) http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822017000400499&lng=en&tlng=en https://doaj.org/toc/1678-9849 1678-9849 doi:10.1590/0037-8682-0023-2017 https://doaj.org/article/4047c81bc4c94769a8b9d723ac395e81 Revista da Sociedade Brasileira de Medicina Tropical, Vol 50, Iss 4, Pp 499-505 Malaria Primaquine HPLC-DAD Validation DBA-2 mice Arctic medicine. Tropical medicine RC955-962 article ftdoajarticles https://doi.org/10.1590/0037-8682-0023-2017 2022-12-31T02:59:00Z Abstract INTRODUCTION: Primaquine (PQ) diphosphate is an 8-aminoquinoline antimalarial drug with unique therapeutic properties. It is the only drug that prevents relapses of Plasmodium vivax or Plasmodium ovale infections. In this study, a fast, sensitive, cost-effective, and robust method for the extraction and high-performance liquid chromatography with diode array ultraviolet detection (HPLC-DAD-UV ) analysis of PQ in the blood plasma was developed and validated. METHODS: After plasma protein precipitation, PQ was obtained by liquid-liquid extraction and analyzed by HPLC-DAD-UV with a modified-silica cyanopropyl column (250mm × 4.6mm i.d. × 5μm) as the stationary phase and a mixture of acetonitrile and 10mM ammonium acetate buffer (pH = 3.80) (45:55) as the mobile phase. The flow rate was 1.0mL·min-1, the oven temperature was 50OC, and absorbance was measured at 264nm. The method was validated for linearity, intra-day and inter-day precision, accuracy, recovery, and robustness. The detection (LOD) and quantification (LOQ) limits were 1.0 and 3.5ng·mL-1, respectively. The method was used to analyze the plasma of female DBA-2 mice treated with 20mg.kg-1 (oral) PQ diphosphate. RESULTS: By combining a simple, low-cost extraction procedure with a sensitive, precise, accurate, and robust method, it was possible to analyze PQ in small volumes of plasma. The new method presents lower LOD and LOQ limits and requires a shorter analysis time and smaller plasma volumes than those of previously reported HPLC methods with DAD-UV detection. CONCLUSIONS: The new validated method is suitable for kinetic studies of PQ in small rodents, including mouse models for the study of malaria. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Revista da Sociedade Brasileira de Medicina Tropical 50 4 499 505 |
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Directory of Open Access Journals: DOAJ Articles |
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English |
topic |
Malaria Primaquine HPLC-DAD Validation DBA-2 mice Arctic medicine. Tropical medicine RC955-962 |
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Malaria Primaquine HPLC-DAD Validation DBA-2 mice Arctic medicine. Tropical medicine RC955-962 Ana Paula Barbosa do Carmo Manoella Borborema Stephan Ribeiro Ana Cecilia Xavier De-Oliveira Francisco Jose Roma Paumgartten Davyson de Lima Moreira A newly validated high-performance liquid chromatography method with diode array ultraviolet detection for analysis of the antimalarial drug primaquine in the blood plasma |
topic_facet |
Malaria Primaquine HPLC-DAD Validation DBA-2 mice Arctic medicine. Tropical medicine RC955-962 |
description |
Abstract INTRODUCTION: Primaquine (PQ) diphosphate is an 8-aminoquinoline antimalarial drug with unique therapeutic properties. It is the only drug that prevents relapses of Plasmodium vivax or Plasmodium ovale infections. In this study, a fast, sensitive, cost-effective, and robust method for the extraction and high-performance liquid chromatography with diode array ultraviolet detection (HPLC-DAD-UV ) analysis of PQ in the blood plasma was developed and validated. METHODS: After plasma protein precipitation, PQ was obtained by liquid-liquid extraction and analyzed by HPLC-DAD-UV with a modified-silica cyanopropyl column (250mm × 4.6mm i.d. × 5μm) as the stationary phase and a mixture of acetonitrile and 10mM ammonium acetate buffer (pH = 3.80) (45:55) as the mobile phase. The flow rate was 1.0mL·min-1, the oven temperature was 50OC, and absorbance was measured at 264nm. The method was validated for linearity, intra-day and inter-day precision, accuracy, recovery, and robustness. The detection (LOD) and quantification (LOQ) limits were 1.0 and 3.5ng·mL-1, respectively. The method was used to analyze the plasma of female DBA-2 mice treated with 20mg.kg-1 (oral) PQ diphosphate. RESULTS: By combining a simple, low-cost extraction procedure with a sensitive, precise, accurate, and robust method, it was possible to analyze PQ in small volumes of plasma. The new method presents lower LOD and LOQ limits and requires a shorter analysis time and smaller plasma volumes than those of previously reported HPLC methods with DAD-UV detection. CONCLUSIONS: The new validated method is suitable for kinetic studies of PQ in small rodents, including mouse models for the study of malaria. |
format |
Article in Journal/Newspaper |
author |
Ana Paula Barbosa do Carmo Manoella Borborema Stephan Ribeiro Ana Cecilia Xavier De-Oliveira Francisco Jose Roma Paumgartten Davyson de Lima Moreira |
author_facet |
Ana Paula Barbosa do Carmo Manoella Borborema Stephan Ribeiro Ana Cecilia Xavier De-Oliveira Francisco Jose Roma Paumgartten Davyson de Lima Moreira |
author_sort |
Ana Paula Barbosa do Carmo |
title |
A newly validated high-performance liquid chromatography method with diode array ultraviolet detection for analysis of the antimalarial drug primaquine in the blood plasma |
title_short |
A newly validated high-performance liquid chromatography method with diode array ultraviolet detection for analysis of the antimalarial drug primaquine in the blood plasma |
title_full |
A newly validated high-performance liquid chromatography method with diode array ultraviolet detection for analysis of the antimalarial drug primaquine in the blood plasma |
title_fullStr |
A newly validated high-performance liquid chromatography method with diode array ultraviolet detection for analysis of the antimalarial drug primaquine in the blood plasma |
title_full_unstemmed |
A newly validated high-performance liquid chromatography method with diode array ultraviolet detection for analysis of the antimalarial drug primaquine in the blood plasma |
title_sort |
newly validated high-performance liquid chromatography method with diode array ultraviolet detection for analysis of the antimalarial drug primaquine in the blood plasma |
publisher |
Sociedade Brasileira de Medicina Tropical (SBMT) |
url |
https://doi.org/10.1590/0037-8682-0023-2017 https://doaj.org/article/4047c81bc4c94769a8b9d723ac395e81 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Revista da Sociedade Brasileira de Medicina Tropical, Vol 50, Iss 4, Pp 499-505 |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822017000400499&lng=en&tlng=en https://doaj.org/toc/1678-9849 1678-9849 doi:10.1590/0037-8682-0023-2017 https://doaj.org/article/4047c81bc4c94769a8b9d723ac395e81 |
op_doi |
https://doi.org/10.1590/0037-8682-0023-2017 |
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Revista da Sociedade Brasileira de Medicina Tropical |
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50 |
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499 |
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