Evaluating 18s-rRNA LAMP and selective whole genome amplification (sWGA) assay in detecting asymptomatic Plasmodium falciparum infections in blood donors
Abstract Background Undesirable consequences of donor Plasmodium falciparum parasitaemia on stored donor blood have been reported. Therefore, it is imperative that all prospective blood donors are screened for P. falciparum infections using sensitive techniques. In this study, the sensitivities of m...
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ftdoajarticles:oai:doaj.org/article:40325139fd0948fd9146b79fb9c44c97 2023-05-15T15:19:08+02:00 Evaluating 18s-rRNA LAMP and selective whole genome amplification (sWGA) assay in detecting asymptomatic Plasmodium falciparum infections in blood donors Enoch Aninagyei Stella Smith-Graham Alex Boye Alexander Egyir-Yawson Desmond Omane Acheampong 2019-06-01T00:00:00Z https://doi.org/10.1186/s12936-019-2850-7 https://doaj.org/article/40325139fd0948fd9146b79fb9c44c97 EN eng BMC http://link.springer.com/article/10.1186/s12936-019-2850-7 https://doaj.org/toc/1475-2875 doi:10.1186/s12936-019-2850-7 1475-2875 https://doaj.org/article/40325139fd0948fd9146b79fb9c44c97 Malaria Journal, Vol 18, Iss 1, Pp 1-11 (2019) 18s-rRNA-LAMP Crude DNA LAMP Purified DNA LAMP Selective whole genome amplification P. falciparum histidine rich protein 2 Plasmodium lactate dehydrogenase Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 2019 ftdoajarticles https://doi.org/10.1186/s12936-019-2850-7 2022-12-30T23:09:49Z Abstract Background Undesirable consequences of donor Plasmodium falciparum parasitaemia on stored donor blood have been reported. Therefore, it is imperative that all prospective blood donors are screened for P. falciparum infections using sensitive techniques. In this study, the sensitivities of microscopy, rapid diagnostic test (RDT), loop-mediated isothermal amplification (LAMP) assay and selective whole genome amplification (sWGA) technique in detecting P. falciparum infections in blood donors was assessed. Methods Randomly selected blood donors from 5 districts in Greater Accra Region of Ghana were screened for asymptomatic P. falciparum infections. Each donor sample was screened with SD Bioline RDT kit for P. falciparum histidine rich protein 2 and Plasmodium lactate dehydrogenase antigens, sWGA and 18s-rRNA LAMP. Crude DNA LAMP (crDNA-LAMP) was compared to purified DNA LAMP (pDNA-LAMP). Results A total of 771 blood donors were screened. The respective overall prevalence of P. falciparum in Ghana by microscopy, RDT, crDNA-LAMP, pDNA-LAMP and sWGA was 7.4%, 11.8%, 16.9%, 17.5% and 18.0%. Using sWGA as the reference test, the sensitivities of microscopy, RDT, crDNA-LAMP and pDNA-LAMP were 41.0% (95% CI 32.7–49.7), 65.5% (95% CI 56.9–73.3), 82.6% (95% CI 75.8–88.3) and 95.7% (95% CI 90.1–98.4), respectively. There was near perfect agreement between LAMP and sWGA (sWGA vs. crDNA-LAMP, κ = 0.87; sWGA vs. pDNA-LAMP, κ = 0.96), while crDNA-LAMP and pDNA-LAMP agreed perfectly (κ = 0.91). Goodness of fit test indicated non-significant difference between the performance of LAMP and sWGA (crDNA-LAMP vs. sWGA: x 2 = 0.71, p = 0.399 and pDNA-LAMP vs. sWGA: x 2 = 0.14, p = 0.707). Finally, compared to sWGA, the performance of LAMP did not differ in detecting sub-microscopic parasitaemia (sWGA vs. crDNA-LAMP: x 2 = 1.12, p = 0.290 and sWGA vs. pDNA-LAMP: x 2 = 0.22, p = 0.638). Conclusions LAMP assay agreed near perfectly with sWGA with non-significant differences in their ability to detect asymptomatic P. falciparum ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Malaria Journal 18 1 |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English |
topic |
18s-rRNA-LAMP Crude DNA LAMP Purified DNA LAMP Selective whole genome amplification P. falciparum histidine rich protein 2 Plasmodium lactate dehydrogenase Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
spellingShingle |
18s-rRNA-LAMP Crude DNA LAMP Purified DNA LAMP Selective whole genome amplification P. falciparum histidine rich protein 2 Plasmodium lactate dehydrogenase Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 Enoch Aninagyei Stella Smith-Graham Alex Boye Alexander Egyir-Yawson Desmond Omane Acheampong Evaluating 18s-rRNA LAMP and selective whole genome amplification (sWGA) assay in detecting asymptomatic Plasmodium falciparum infections in blood donors |
topic_facet |
18s-rRNA-LAMP Crude DNA LAMP Purified DNA LAMP Selective whole genome amplification P. falciparum histidine rich protein 2 Plasmodium lactate dehydrogenase Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
Abstract Background Undesirable consequences of donor Plasmodium falciparum parasitaemia on stored donor blood have been reported. Therefore, it is imperative that all prospective blood donors are screened for P. falciparum infections using sensitive techniques. In this study, the sensitivities of microscopy, rapid diagnostic test (RDT), loop-mediated isothermal amplification (LAMP) assay and selective whole genome amplification (sWGA) technique in detecting P. falciparum infections in blood donors was assessed. Methods Randomly selected blood donors from 5 districts in Greater Accra Region of Ghana were screened for asymptomatic P. falciparum infections. Each donor sample was screened with SD Bioline RDT kit for P. falciparum histidine rich protein 2 and Plasmodium lactate dehydrogenase antigens, sWGA and 18s-rRNA LAMP. Crude DNA LAMP (crDNA-LAMP) was compared to purified DNA LAMP (pDNA-LAMP). Results A total of 771 blood donors were screened. The respective overall prevalence of P. falciparum in Ghana by microscopy, RDT, crDNA-LAMP, pDNA-LAMP and sWGA was 7.4%, 11.8%, 16.9%, 17.5% and 18.0%. Using sWGA as the reference test, the sensitivities of microscopy, RDT, crDNA-LAMP and pDNA-LAMP were 41.0% (95% CI 32.7–49.7), 65.5% (95% CI 56.9–73.3), 82.6% (95% CI 75.8–88.3) and 95.7% (95% CI 90.1–98.4), respectively. There was near perfect agreement between LAMP and sWGA (sWGA vs. crDNA-LAMP, κ = 0.87; sWGA vs. pDNA-LAMP, κ = 0.96), while crDNA-LAMP and pDNA-LAMP agreed perfectly (κ = 0.91). Goodness of fit test indicated non-significant difference between the performance of LAMP and sWGA (crDNA-LAMP vs. sWGA: x 2 = 0.71, p = 0.399 and pDNA-LAMP vs. sWGA: x 2 = 0.14, p = 0.707). Finally, compared to sWGA, the performance of LAMP did not differ in detecting sub-microscopic parasitaemia (sWGA vs. crDNA-LAMP: x 2 = 1.12, p = 0.290 and sWGA vs. pDNA-LAMP: x 2 = 0.22, p = 0.638). Conclusions LAMP assay agreed near perfectly with sWGA with non-significant differences in their ability to detect asymptomatic P. falciparum ... |
format |
Article in Journal/Newspaper |
author |
Enoch Aninagyei Stella Smith-Graham Alex Boye Alexander Egyir-Yawson Desmond Omane Acheampong |
author_facet |
Enoch Aninagyei Stella Smith-Graham Alex Boye Alexander Egyir-Yawson Desmond Omane Acheampong |
author_sort |
Enoch Aninagyei |
title |
Evaluating 18s-rRNA LAMP and selective whole genome amplification (sWGA) assay in detecting asymptomatic Plasmodium falciparum infections in blood donors |
title_short |
Evaluating 18s-rRNA LAMP and selective whole genome amplification (sWGA) assay in detecting asymptomatic Plasmodium falciparum infections in blood donors |
title_full |
Evaluating 18s-rRNA LAMP and selective whole genome amplification (sWGA) assay in detecting asymptomatic Plasmodium falciparum infections in blood donors |
title_fullStr |
Evaluating 18s-rRNA LAMP and selective whole genome amplification (sWGA) assay in detecting asymptomatic Plasmodium falciparum infections in blood donors |
title_full_unstemmed |
Evaluating 18s-rRNA LAMP and selective whole genome amplification (sWGA) assay in detecting asymptomatic Plasmodium falciparum infections in blood donors |
title_sort |
evaluating 18s-rrna lamp and selective whole genome amplification (swga) assay in detecting asymptomatic plasmodium falciparum infections in blood donors |
publisher |
BMC |
publishDate |
2019 |
url |
https://doi.org/10.1186/s12936-019-2850-7 https://doaj.org/article/40325139fd0948fd9146b79fb9c44c97 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Malaria Journal, Vol 18, Iss 1, Pp 1-11 (2019) |
op_relation |
http://link.springer.com/article/10.1186/s12936-019-2850-7 https://doaj.org/toc/1475-2875 doi:10.1186/s12936-019-2850-7 1475-2875 https://doaj.org/article/40325139fd0948fd9146b79fb9c44c97 |
op_doi |
https://doi.org/10.1186/s12936-019-2850-7 |
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Malaria Journal |
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18 |
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1766349320458600448 |