Eomesodermin of atlantic salmon: an important regulator of cytolytic gene and interferon gamma expression in spleen lymphocytes.
Eomesodermin (Eomes), a T-bet homologue expressed in activated CD8+T cells was recently proposed to act as a master regulator of cytotoxic CD8+ T cell effector function and offers an exciting avenue for future exploration. Here, we have identified and characterized the full-length Atlantic salmon Eo...
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ftdoajarticles:oai:doaj.org/article:3fae08daf4454c72a631bc1ce459b819 2023-05-15T15:30:30+02:00 Eomesodermin of atlantic salmon: an important regulator of cytolytic gene and interferon gamma expression in spleen lymphocytes. Jaya Kumari Jarl Bøgwald Roy A Dalmo 2013-01-01T00:00:00Z https://doi.org/10.1371/journal.pone.0055893 https://doaj.org/article/3fae08daf4454c72a631bc1ce459b819 EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC3567031?pdf=render https://doaj.org/toc/1932-6203 1932-6203 doi:10.1371/journal.pone.0055893 https://doaj.org/article/3fae08daf4454c72a631bc1ce459b819 PLoS ONE, Vol 8, Iss 2, p e55893 (2013) Medicine R Science Q article 2013 ftdoajarticles https://doi.org/10.1371/journal.pone.0055893 2022-12-30T23:06:42Z Eomesodermin (Eomes), a T-bet homologue expressed in activated CD8+T cells was recently proposed to act as a master regulator of cytotoxic CD8+ T cell effector function and offers an exciting avenue for future exploration. Here, we have identified and characterized the full-length Atlantic salmon Eomes cDNA (2477 bp). Promoter analysis of the salmon Eomes showed the presence of important putative transcription binding sites like SP1, FOXO, Oct-1, SMAD, STAT, IRF, and Ets-1. The basal core region responsible for the promoter activity was located between base -199 and +59. Quantitative PCR analysis revealed that the Atlantic salmon Eomes was ubiquitously expressed in all the tissues studied but strongly expressed in the ovary, spleen, brain, and the head kidney. Moreover, the involvement of Eomes in Atlantic salmon immune response and its relation with the cytolytic activity was demonstrated by investigating the early time dependent expression profile of Eomes and CD8α followed by high interferon gamma (IFN-γ) and granzyme A expression during challenge with live Aeromonas salmonicida and Infectious Pancreatic Necrosis (IPN) virus. Therefore, we further analyzed the regulated expression and function of this transcription factor in spleen lymphocytes. Overexpression of Eomes induced IFN-γ, and granzyme A expression but not perforin expression, whereas small interfering RNA (siRNA) mediated suppression of Eomes expression led to significantly reduced IFN-γ production. Thus, Eomes may be critical in cytolytic gene expression and function in fish similar to mammals. Furthermore, IFN-α, and mitogens induced Eomes expression. Taken together, this is the first study on the promoter activity and regulatory role of Eomes in fish. Article in Journal/Newspaper Atlantic salmon Directory of Open Access Journals: DOAJ Articles PLoS ONE 8 2 e55893 |
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Medicine R Science Q |
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Medicine R Science Q Jaya Kumari Jarl Bøgwald Roy A Dalmo Eomesodermin of atlantic salmon: an important regulator of cytolytic gene and interferon gamma expression in spleen lymphocytes. |
topic_facet |
Medicine R Science Q |
description |
Eomesodermin (Eomes), a T-bet homologue expressed in activated CD8+T cells was recently proposed to act as a master regulator of cytotoxic CD8+ T cell effector function and offers an exciting avenue for future exploration. Here, we have identified and characterized the full-length Atlantic salmon Eomes cDNA (2477 bp). Promoter analysis of the salmon Eomes showed the presence of important putative transcription binding sites like SP1, FOXO, Oct-1, SMAD, STAT, IRF, and Ets-1. The basal core region responsible for the promoter activity was located between base -199 and +59. Quantitative PCR analysis revealed that the Atlantic salmon Eomes was ubiquitously expressed in all the tissues studied but strongly expressed in the ovary, spleen, brain, and the head kidney. Moreover, the involvement of Eomes in Atlantic salmon immune response and its relation with the cytolytic activity was demonstrated by investigating the early time dependent expression profile of Eomes and CD8α followed by high interferon gamma (IFN-γ) and granzyme A expression during challenge with live Aeromonas salmonicida and Infectious Pancreatic Necrosis (IPN) virus. Therefore, we further analyzed the regulated expression and function of this transcription factor in spleen lymphocytes. Overexpression of Eomes induced IFN-γ, and granzyme A expression but not perforin expression, whereas small interfering RNA (siRNA) mediated suppression of Eomes expression led to significantly reduced IFN-γ production. Thus, Eomes may be critical in cytolytic gene expression and function in fish similar to mammals. Furthermore, IFN-α, and mitogens induced Eomes expression. Taken together, this is the first study on the promoter activity and regulatory role of Eomes in fish. |
format |
Article in Journal/Newspaper |
author |
Jaya Kumari Jarl Bøgwald Roy A Dalmo |
author_facet |
Jaya Kumari Jarl Bøgwald Roy A Dalmo |
author_sort |
Jaya Kumari |
title |
Eomesodermin of atlantic salmon: an important regulator of cytolytic gene and interferon gamma expression in spleen lymphocytes. |
title_short |
Eomesodermin of atlantic salmon: an important regulator of cytolytic gene and interferon gamma expression in spleen lymphocytes. |
title_full |
Eomesodermin of atlantic salmon: an important regulator of cytolytic gene and interferon gamma expression in spleen lymphocytes. |
title_fullStr |
Eomesodermin of atlantic salmon: an important regulator of cytolytic gene and interferon gamma expression in spleen lymphocytes. |
title_full_unstemmed |
Eomesodermin of atlantic salmon: an important regulator of cytolytic gene and interferon gamma expression in spleen lymphocytes. |
title_sort |
eomesodermin of atlantic salmon: an important regulator of cytolytic gene and interferon gamma expression in spleen lymphocytes. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2013 |
url |
https://doi.org/10.1371/journal.pone.0055893 https://doaj.org/article/3fae08daf4454c72a631bc1ce459b819 |
genre |
Atlantic salmon |
genre_facet |
Atlantic salmon |
op_source |
PLoS ONE, Vol 8, Iss 2, p e55893 (2013) |
op_relation |
http://europepmc.org/articles/PMC3567031?pdf=render https://doaj.org/toc/1932-6203 1932-6203 doi:10.1371/journal.pone.0055893 https://doaj.org/article/3fae08daf4454c72a631bc1ce459b819 |
op_doi |
https://doi.org/10.1371/journal.pone.0055893 |
container_title |
PLoS ONE |
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8 |
container_issue |
2 |
container_start_page |
e55893 |
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