Genome sequencing reveals coinfection by multiple chikungunya virus genotypes in a recent outbreak in Brazil.
Chikungunya virus (CHIKV) is an RNA virus from the Togaviridae family transmitted by mosquitoes in both sylvatic and urban cycles. In humans, CHIKV infection leads to a febrile illness, denominated Chikungunya fever (CHIKF), commonly associated with more intense and debilitating outcomes. CHIKV arri...
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ftdoajarticles:oai:doaj.org/article:3f8c568ad0f040df8f53e2991c70776d 2023-11-12T04:13:19+01:00 Genome sequencing reveals coinfection by multiple chikungunya virus genotypes in a recent outbreak in Brazil. Lais Ceschini Machado Mariana Carolina de Morais-Sobral Tulio de Lima Campos Mylena Ribeiro Pereira Maria de Fátima Pessoa Militão de Albuquerque Clément Gilbert Rafael Freitas Oliveira Franca Gabriel Luz Wallau 2019-05-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0007332 https://doaj.org/article/3f8c568ad0f040df8f53e2991c70776d EN eng Public Library of Science (PLoS) https://doi.org/10.1371/journal.pntd.0007332 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0007332 https://doaj.org/article/3f8c568ad0f040df8f53e2991c70776d PLoS Neglected Tropical Diseases, Vol 13, Iss 5, p e0007332 (2019) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2019 ftdoajarticles https://doi.org/10.1371/journal.pntd.0007332 2023-10-15T00:37:08Z Chikungunya virus (CHIKV) is an RNA virus from the Togaviridae family transmitted by mosquitoes in both sylvatic and urban cycles. In humans, CHIKV infection leads to a febrile illness, denominated Chikungunya fever (CHIKF), commonly associated with more intense and debilitating outcomes. CHIKV arrived in Brazil in 2014 through two independent introductions: the Asian/Caribbean genotype entered through the North region and the African ECSA genotype was imported through the Northeast region. Following their initial introduction, both genotypes established their urban cycle among large naive human populations causing several outbreaks in the Americas. Here, we sequenced CHIKV genomes from a recent outbreak in the Northeast region of Brazil, employing an in-house developed Next-Generation Sequencing (NGS) protocol capable of directly detecting multiple known CHIKV genotypes from clinical positive samples. Our results demonstrate that both Asian/Caribbean and ECSA genotypes expanded their ranges, reaching cocirculation in the Northeast region of Brazil. In addition, our NGS data supports the findings of simultaneous infection by these two genotypes, suggesting that coinfection might be more common than previously thought in highly endemic areas. Future efforts to understand CHIKV epidemiology should thus take into consideration the possibility of coinfection by different genotypes in the human population. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 13 5 e0007332 |
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Directory of Open Access Journals: DOAJ Articles |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Lais Ceschini Machado Mariana Carolina de Morais-Sobral Tulio de Lima Campos Mylena Ribeiro Pereira Maria de Fátima Pessoa Militão de Albuquerque Clément Gilbert Rafael Freitas Oliveira Franca Gabriel Luz Wallau Genome sequencing reveals coinfection by multiple chikungunya virus genotypes in a recent outbreak in Brazil. |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Chikungunya virus (CHIKV) is an RNA virus from the Togaviridae family transmitted by mosquitoes in both sylvatic and urban cycles. In humans, CHIKV infection leads to a febrile illness, denominated Chikungunya fever (CHIKF), commonly associated with more intense and debilitating outcomes. CHIKV arrived in Brazil in 2014 through two independent introductions: the Asian/Caribbean genotype entered through the North region and the African ECSA genotype was imported through the Northeast region. Following their initial introduction, both genotypes established their urban cycle among large naive human populations causing several outbreaks in the Americas. Here, we sequenced CHIKV genomes from a recent outbreak in the Northeast region of Brazil, employing an in-house developed Next-Generation Sequencing (NGS) protocol capable of directly detecting multiple known CHIKV genotypes from clinical positive samples. Our results demonstrate that both Asian/Caribbean and ECSA genotypes expanded their ranges, reaching cocirculation in the Northeast region of Brazil. In addition, our NGS data supports the findings of simultaneous infection by these two genotypes, suggesting that coinfection might be more common than previously thought in highly endemic areas. Future efforts to understand CHIKV epidemiology should thus take into consideration the possibility of coinfection by different genotypes in the human population. |
format |
Article in Journal/Newspaper |
author |
Lais Ceschini Machado Mariana Carolina de Morais-Sobral Tulio de Lima Campos Mylena Ribeiro Pereira Maria de Fátima Pessoa Militão de Albuquerque Clément Gilbert Rafael Freitas Oliveira Franca Gabriel Luz Wallau |
author_facet |
Lais Ceschini Machado Mariana Carolina de Morais-Sobral Tulio de Lima Campos Mylena Ribeiro Pereira Maria de Fátima Pessoa Militão de Albuquerque Clément Gilbert Rafael Freitas Oliveira Franca Gabriel Luz Wallau |
author_sort |
Lais Ceschini Machado |
title |
Genome sequencing reveals coinfection by multiple chikungunya virus genotypes in a recent outbreak in Brazil. |
title_short |
Genome sequencing reveals coinfection by multiple chikungunya virus genotypes in a recent outbreak in Brazil. |
title_full |
Genome sequencing reveals coinfection by multiple chikungunya virus genotypes in a recent outbreak in Brazil. |
title_fullStr |
Genome sequencing reveals coinfection by multiple chikungunya virus genotypes in a recent outbreak in Brazil. |
title_full_unstemmed |
Genome sequencing reveals coinfection by multiple chikungunya virus genotypes in a recent outbreak in Brazil. |
title_sort |
genome sequencing reveals coinfection by multiple chikungunya virus genotypes in a recent outbreak in brazil. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2019 |
url |
https://doi.org/10.1371/journal.pntd.0007332 https://doaj.org/article/3f8c568ad0f040df8f53e2991c70776d |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 13, Iss 5, p e0007332 (2019) |
op_relation |
https://doi.org/10.1371/journal.pntd.0007332 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0007332 https://doaj.org/article/3f8c568ad0f040df8f53e2991c70776d |
op_doi |
https://doi.org/10.1371/journal.pntd.0007332 |
container_title |
PLOS Neglected Tropical Diseases |
container_volume |
13 |
container_issue |
5 |
container_start_page |
e0007332 |
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