Escherichia coli vacuolating factor, involved in avian cellulitis, induces actin contraction and binds to cytoskeleton proteins in fibroblasts
Abstract Background Avian pathogenic Escherichia coli (APEC) isolated from avian cellulitis lesions produces a toxin, named Escherichia coli vacuolating factor (ECVF), that causes cell vacuolization and induces inflammatory response in broiler chicken. Methods We investigated the intracellular activ...
Published in: | Journal of Venomous Animals and Toxins including Tropical Diseases |
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ftdoajarticles:oai:doaj.org/article:3f8afea7c62546ddb67af9e69140d30c 2023-05-15T15:07:24+02:00 Escherichia coli vacuolating factor, involved in avian cellulitis, induces actin contraction and binds to cytoskeleton proteins in fibroblasts Annelize Zambon Barbosa Aragão Natália Galdi Quel Paulo Pinto Joazeiro Tomomasa Yano 2021-03-01T00:00:00Z https://doi.org/10.1590/1678-9199-jvatitd-2020-0106 https://doaj.org/article/3f8afea7c62546ddb67af9e69140d30c EN eng SciELO http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992021000100303&tlng=en http://www.scielo.br/pdf/jvatitd/v27/1678-9199-jvatitd-27-e20200106.pdf https://doaj.org/toc/1678-9199 1678-9199 doi:10.1590/1678-9199-jvatitd-2020-0106 https://doaj.org/article/3f8afea7c62546ddb67af9e69140d30c Journal of Venomous Animals and Toxins including Tropical Diseases, Vol 27 (2021) Escherichia coli Escherichia coli vacuolating factor (ECVF) Avian pathogenic Escherichia coli (APEC) Cytoskeleton Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 article 2021 ftdoajarticles https://doi.org/10.1590/1678-9199-jvatitd-2020-0106 2022-12-31T16:06:47Z Abstract Background Avian pathogenic Escherichia coli (APEC) isolated from avian cellulitis lesions produces a toxin, named Escherichia coli vacuolating factor (ECVF), that causes cell vacuolization and induces inflammatory response in broiler chicken. Methods We investigated the intracellular activities of ECVF in avian fibroblasts using fluorescence staining, electron microscopy, MTT and LDH measurements. As ECVF act specifically in avian cells, we performed blotting assay followed by mass spectrometry to better understand its initial intracellular protein recognition. Results ECVF induced actin contraction, mitochondrial damage and membrane permeability alterations. Ultrastructural analysis showed intracellular alterations, as nuclear lobulation and the presence of degraded structures inside the vacuoles. Moreover, ECVF induced cell death in fibroblasts. ECVF-biotin associates to at least two proteins only in avian cell lysates: alpha-actinin 4 and vinculin, both involved in cytoskeleton structure. Conclusion These findings demonstrated that ECVF plays an important role in avian cellulitis, markedly in initial steps of infection. Taken together, the results place this toxin as a target for drug and/or vaccine development, instead of the use of large amounts antibiotics. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Journal of Venomous Animals and Toxins including Tropical Diseases 27 |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English |
topic |
Escherichia coli Escherichia coli vacuolating factor (ECVF) Avian pathogenic Escherichia coli (APEC) Cytoskeleton Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 |
spellingShingle |
Escherichia coli Escherichia coli vacuolating factor (ECVF) Avian pathogenic Escherichia coli (APEC) Cytoskeleton Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 Annelize Zambon Barbosa Aragão Natália Galdi Quel Paulo Pinto Joazeiro Tomomasa Yano Escherichia coli vacuolating factor, involved in avian cellulitis, induces actin contraction and binds to cytoskeleton proteins in fibroblasts |
topic_facet |
Escherichia coli Escherichia coli vacuolating factor (ECVF) Avian pathogenic Escherichia coli (APEC) Cytoskeleton Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 |
description |
Abstract Background Avian pathogenic Escherichia coli (APEC) isolated from avian cellulitis lesions produces a toxin, named Escherichia coli vacuolating factor (ECVF), that causes cell vacuolization and induces inflammatory response in broiler chicken. Methods We investigated the intracellular activities of ECVF in avian fibroblasts using fluorescence staining, electron microscopy, MTT and LDH measurements. As ECVF act specifically in avian cells, we performed blotting assay followed by mass spectrometry to better understand its initial intracellular protein recognition. Results ECVF induced actin contraction, mitochondrial damage and membrane permeability alterations. Ultrastructural analysis showed intracellular alterations, as nuclear lobulation and the presence of degraded structures inside the vacuoles. Moreover, ECVF induced cell death in fibroblasts. ECVF-biotin associates to at least two proteins only in avian cell lysates: alpha-actinin 4 and vinculin, both involved in cytoskeleton structure. Conclusion These findings demonstrated that ECVF plays an important role in avian cellulitis, markedly in initial steps of infection. Taken together, the results place this toxin as a target for drug and/or vaccine development, instead of the use of large amounts antibiotics. |
format |
Article in Journal/Newspaper |
author |
Annelize Zambon Barbosa Aragão Natália Galdi Quel Paulo Pinto Joazeiro Tomomasa Yano |
author_facet |
Annelize Zambon Barbosa Aragão Natália Galdi Quel Paulo Pinto Joazeiro Tomomasa Yano |
author_sort |
Annelize Zambon Barbosa Aragão |
title |
Escherichia coli vacuolating factor, involved in avian cellulitis, induces actin contraction and binds to cytoskeleton proteins in fibroblasts |
title_short |
Escherichia coli vacuolating factor, involved in avian cellulitis, induces actin contraction and binds to cytoskeleton proteins in fibroblasts |
title_full |
Escherichia coli vacuolating factor, involved in avian cellulitis, induces actin contraction and binds to cytoskeleton proteins in fibroblasts |
title_fullStr |
Escherichia coli vacuolating factor, involved in avian cellulitis, induces actin contraction and binds to cytoskeleton proteins in fibroblasts |
title_full_unstemmed |
Escherichia coli vacuolating factor, involved in avian cellulitis, induces actin contraction and binds to cytoskeleton proteins in fibroblasts |
title_sort |
escherichia coli vacuolating factor, involved in avian cellulitis, induces actin contraction and binds to cytoskeleton proteins in fibroblasts |
publisher |
SciELO |
publishDate |
2021 |
url |
https://doi.org/10.1590/1678-9199-jvatitd-2020-0106 https://doaj.org/article/3f8afea7c62546ddb67af9e69140d30c |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Journal of Venomous Animals and Toxins including Tropical Diseases, Vol 27 (2021) |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992021000100303&tlng=en http://www.scielo.br/pdf/jvatitd/v27/1678-9199-jvatitd-27-e20200106.pdf https://doaj.org/toc/1678-9199 1678-9199 doi:10.1590/1678-9199-jvatitd-2020-0106 https://doaj.org/article/3f8afea7c62546ddb67af9e69140d30c |
op_doi |
https://doi.org/10.1590/1678-9199-jvatitd-2020-0106 |
container_title |
Journal of Venomous Animals and Toxins including Tropical Diseases |
container_volume |
27 |
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