Serological evaluation of Mycobacterium ulcerans antigens identified by comparative genomics.
A specific and sensitive serodiagnostic test for Mycobacterium ulcerans infection would greatly assist the diagnosis of Buruli ulcer and would also facilitate seroepidemiological surveys. By comparative genomics, we identified 45 potential M. ulcerans specific proteins, of which we were able to expr...
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ftdoajarticles:oai:doaj.org/article:3f778c8f06644033a3b0cfede9ce041d 2023-05-15T15:09:21+02:00 Serological evaluation of Mycobacterium ulcerans antigens identified by comparative genomics. Sacha J Pidot Jessica L Porter Laurent Marsollier Annick Chauty Florence Migot-Nabias Cyril Badaut Angèle Bénard Marie-Therese Ruf Torsten Seemann Paul D R Johnson John K Davies Grant A Jenkin Gerd Pluschke Timothy P Stinear 2010-01-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0000872 https://doaj.org/article/3f778c8f06644033a3b0cfede9ce041d EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC2970529?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0000872 https://doaj.org/article/3f778c8f06644033a3b0cfede9ce041d PLoS Neglected Tropical Diseases, Vol 4, Iss 11, p e872 (2010) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2010 ftdoajarticles https://doi.org/10.1371/journal.pntd.0000872 2022-12-31T11:08:58Z A specific and sensitive serodiagnostic test for Mycobacterium ulcerans infection would greatly assist the diagnosis of Buruli ulcer and would also facilitate seroepidemiological surveys. By comparative genomics, we identified 45 potential M. ulcerans specific proteins, of which we were able to express and purify 33 in E. coli. Sera from 30 confirmed Buruli ulcer patients, 24 healthy controls from the same endemic region and 30 healthy controls from a non-endemic region in Benin were screened for antibody responses to these specific proteins by ELISA. Serum IgG responses of Buruli ulcer patients were highly variable, however, seven proteins (MUP045, MUP057, MUL_0513, Hsp65, and the polyketide synthase domains ER, AT propionate, and KR A) showed a significant difference between patient and non-endemic control antibody responses. However, when sera from the healthy control subjects living in the same Buruli ulcer endemic area as the patients were examined, none of the proteins were able to discriminate between these two groups. Nevertheless, six of the seven proteins showed an ability to distinguish people living in an endemic area from those in a non-endemic area with an average sensitivity of 69% and specificity of 88%, suggesting exposure to M. ulcerans. Further validation of these six proteins is now underway to assess their suitability for use in Buruli ulcer seroepidemiological studies. Such studies are urgently needed to assist efforts to uncover environmental reservoirs and understand transmission pathways of the M. ulcerans. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLoS Neglected Tropical Diseases 4 11 e872 |
institution |
Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
spellingShingle |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Sacha J Pidot Jessica L Porter Laurent Marsollier Annick Chauty Florence Migot-Nabias Cyril Badaut Angèle Bénard Marie-Therese Ruf Torsten Seemann Paul D R Johnson John K Davies Grant A Jenkin Gerd Pluschke Timothy P Stinear Serological evaluation of Mycobacterium ulcerans antigens identified by comparative genomics. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
A specific and sensitive serodiagnostic test for Mycobacterium ulcerans infection would greatly assist the diagnosis of Buruli ulcer and would also facilitate seroepidemiological surveys. By comparative genomics, we identified 45 potential M. ulcerans specific proteins, of which we were able to express and purify 33 in E. coli. Sera from 30 confirmed Buruli ulcer patients, 24 healthy controls from the same endemic region and 30 healthy controls from a non-endemic region in Benin were screened for antibody responses to these specific proteins by ELISA. Serum IgG responses of Buruli ulcer patients were highly variable, however, seven proteins (MUP045, MUP057, MUL_0513, Hsp65, and the polyketide synthase domains ER, AT propionate, and KR A) showed a significant difference between patient and non-endemic control antibody responses. However, when sera from the healthy control subjects living in the same Buruli ulcer endemic area as the patients were examined, none of the proteins were able to discriminate between these two groups. Nevertheless, six of the seven proteins showed an ability to distinguish people living in an endemic area from those in a non-endemic area with an average sensitivity of 69% and specificity of 88%, suggesting exposure to M. ulcerans. Further validation of these six proteins is now underway to assess their suitability for use in Buruli ulcer seroepidemiological studies. Such studies are urgently needed to assist efforts to uncover environmental reservoirs and understand transmission pathways of the M. ulcerans. |
format |
Article in Journal/Newspaper |
author |
Sacha J Pidot Jessica L Porter Laurent Marsollier Annick Chauty Florence Migot-Nabias Cyril Badaut Angèle Bénard Marie-Therese Ruf Torsten Seemann Paul D R Johnson John K Davies Grant A Jenkin Gerd Pluschke Timothy P Stinear |
author_facet |
Sacha J Pidot Jessica L Porter Laurent Marsollier Annick Chauty Florence Migot-Nabias Cyril Badaut Angèle Bénard Marie-Therese Ruf Torsten Seemann Paul D R Johnson John K Davies Grant A Jenkin Gerd Pluschke Timothy P Stinear |
author_sort |
Sacha J Pidot |
title |
Serological evaluation of Mycobacterium ulcerans antigens identified by comparative genomics. |
title_short |
Serological evaluation of Mycobacterium ulcerans antigens identified by comparative genomics. |
title_full |
Serological evaluation of Mycobacterium ulcerans antigens identified by comparative genomics. |
title_fullStr |
Serological evaluation of Mycobacterium ulcerans antigens identified by comparative genomics. |
title_full_unstemmed |
Serological evaluation of Mycobacterium ulcerans antigens identified by comparative genomics. |
title_sort |
serological evaluation of mycobacterium ulcerans antigens identified by comparative genomics. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2010 |
url |
https://doi.org/10.1371/journal.pntd.0000872 https://doaj.org/article/3f778c8f06644033a3b0cfede9ce041d |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 4, Iss 11, p e872 (2010) |
op_relation |
http://europepmc.org/articles/PMC2970529?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0000872 https://doaj.org/article/3f778c8f06644033a3b0cfede9ce041d |
op_doi |
https://doi.org/10.1371/journal.pntd.0000872 |
container_title |
PLoS Neglected Tropical Diseases |
container_volume |
4 |
container_issue |
11 |
container_start_page |
e872 |
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