Diagnosis of hepatitis C virus in Brazilian blood donors using a reverse transcriptase nested polymerase chain reaction: comparison with enzyme immunoassay and recombinant protein immunoblot assay
Screening blood donations for anti-HCV antibodies and alanine aminotransferase (ALT) serum levels generally prevents the transmission of hepatitis C virus (HCV) by transfusion. The aim of the present study was to evaluate the efficiency of the enzyme immunoassay (EIA) screening policy in identifying...
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Universidade de São Paulo (USP)
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ftdoajarticles:oai:doaj.org/article:3f461127a1c44c128de91a71e95af3cf 2024-09-09T19:28:39+00:00 Diagnosis of hepatitis C virus in Brazilian blood donors using a reverse transcriptase nested polymerase chain reaction: comparison with enzyme immunoassay and recombinant protein immunoblot assay Neiva S. L. GONÇALES Fernando F. COSTA José VASSALLO Fernando L. GONÇALES JR. 2000-10-01T00:00:00Z https://doaj.org/article/3f461127a1c44c128de91a71e95af3cf EN eng Universidade de São Paulo (USP) http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0036-46652000000500005&tlng=en https://doaj.org/toc/1678-9946 1678-9946 https://doaj.org/article/3f461127a1c44c128de91a71e95af3cf Revista do Instituto de Medicina Tropical de São Paulo, Vol 42, Iss 5, Pp 263-267 (2000) Anti-HCV Blood donors Viral hepatitis Hepatitis C Brazil PCR RIBA Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 2000 ftdoajarticles 2024-08-05T17:49:31Z Screening blood donations for anti-HCV antibodies and alanine aminotransferase (ALT) serum levels generally prevents the transmission of hepatitis C virus (HCV) by transfusion. The aim of the present study was to evaluate the efficiency of the enzyme immunoassay (EIA) screening policy in identifying potentially infectious blood donors capable to transmit hepatitis C through blood transfusion. We have used a reverse transcriptase (RT)-nested polymerase chain reaction (PCR) to investigate the presence of HCV-RNA in blood donors. The prevalence of HCV-RNA positive individuals was compared with the recombinant immunoblot assay (RIBA-2) results in order to assess the usefulness of both tests as confirmatory assays. Both tests results were also compared with the EIA-2 OD/C ratio (optical densities of the samples divided by the cut off value). ALT results were expressed as the ALT quotient (qALT), calculated dividing the ALT value of the samples by the maximum normal value (53UI/l) for the method. Donors (n=178) were divided into five groups according to their EIA anti-HCV status and qALT: group A (EIA > or = 3, ALT<1), group B (EIA > or = 3, ALT>1), group C (1<=EIA<3, ALT<1), group D (1<=EIA<3, ALT>1) and group E (EIA<=0.7). HCV sequences were detected by RT-nested PCR, using primers for the most conserved region of viral genome. RIBA-2 was applied to the same samples. In group A (n=6), all samples were positive by RT-nested PCR and RIBA-2. Among 124 samples in group B, 120 (96.8%) were RIBA-2 positive and 4 (3.2%) were RIBA-2 indeterminate but were seropositive for antigen c22.3. In group B, 109 (87.9%) of the RIBA-2 positive samples were also RT-nested PCR positive, as well as were all RIBA-2 indeterminate samples. In group C, all samples (n=9) were RT-nested PCR negative: 4 (44.4%) were also RIBA-2 negative, 4 (44.4%) were RIBA-2 positive and 1 (11.1%) was RIBA-2 indeterminate. HCV-RNA was detected by RT-nested PCR in 3 (37.5%) out of 8 samples in group D. Only one of them was also ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Eia ENVELOPE(7.755,7.755,63.024,63.024) |
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Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English |
topic |
Anti-HCV Blood donors Viral hepatitis Hepatitis C Brazil PCR RIBA Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
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Anti-HCV Blood donors Viral hepatitis Hepatitis C Brazil PCR RIBA Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 Neiva S. L. GONÇALES Fernando F. COSTA José VASSALLO Fernando L. GONÇALES JR. Diagnosis of hepatitis C virus in Brazilian blood donors using a reverse transcriptase nested polymerase chain reaction: comparison with enzyme immunoassay and recombinant protein immunoblot assay |
topic_facet |
Anti-HCV Blood donors Viral hepatitis Hepatitis C Brazil PCR RIBA Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
Screening blood donations for anti-HCV antibodies and alanine aminotransferase (ALT) serum levels generally prevents the transmission of hepatitis C virus (HCV) by transfusion. The aim of the present study was to evaluate the efficiency of the enzyme immunoassay (EIA) screening policy in identifying potentially infectious blood donors capable to transmit hepatitis C through blood transfusion. We have used a reverse transcriptase (RT)-nested polymerase chain reaction (PCR) to investigate the presence of HCV-RNA in blood donors. The prevalence of HCV-RNA positive individuals was compared with the recombinant immunoblot assay (RIBA-2) results in order to assess the usefulness of both tests as confirmatory assays. Both tests results were also compared with the EIA-2 OD/C ratio (optical densities of the samples divided by the cut off value). ALT results were expressed as the ALT quotient (qALT), calculated dividing the ALT value of the samples by the maximum normal value (53UI/l) for the method. Donors (n=178) were divided into five groups according to their EIA anti-HCV status and qALT: group A (EIA > or = 3, ALT<1), group B (EIA > or = 3, ALT>1), group C (1<=EIA<3, ALT<1), group D (1<=EIA<3, ALT>1) and group E (EIA<=0.7). HCV sequences were detected by RT-nested PCR, using primers for the most conserved region of viral genome. RIBA-2 was applied to the same samples. In group A (n=6), all samples were positive by RT-nested PCR and RIBA-2. Among 124 samples in group B, 120 (96.8%) were RIBA-2 positive and 4 (3.2%) were RIBA-2 indeterminate but were seropositive for antigen c22.3. In group B, 109 (87.9%) of the RIBA-2 positive samples were also RT-nested PCR positive, as well as were all RIBA-2 indeterminate samples. In group C, all samples (n=9) were RT-nested PCR negative: 4 (44.4%) were also RIBA-2 negative, 4 (44.4%) were RIBA-2 positive and 1 (11.1%) was RIBA-2 indeterminate. HCV-RNA was detected by RT-nested PCR in 3 (37.5%) out of 8 samples in group D. Only one of them was also ... |
format |
Article in Journal/Newspaper |
author |
Neiva S. L. GONÇALES Fernando F. COSTA José VASSALLO Fernando L. GONÇALES JR. |
author_facet |
Neiva S. L. GONÇALES Fernando F. COSTA José VASSALLO Fernando L. GONÇALES JR. |
author_sort |
Neiva S. L. GONÇALES |
title |
Diagnosis of hepatitis C virus in Brazilian blood donors using a reverse transcriptase nested polymerase chain reaction: comparison with enzyme immunoassay and recombinant protein immunoblot assay |
title_short |
Diagnosis of hepatitis C virus in Brazilian blood donors using a reverse transcriptase nested polymerase chain reaction: comparison with enzyme immunoassay and recombinant protein immunoblot assay |
title_full |
Diagnosis of hepatitis C virus in Brazilian blood donors using a reverse transcriptase nested polymerase chain reaction: comparison with enzyme immunoassay and recombinant protein immunoblot assay |
title_fullStr |
Diagnosis of hepatitis C virus in Brazilian blood donors using a reverse transcriptase nested polymerase chain reaction: comparison with enzyme immunoassay and recombinant protein immunoblot assay |
title_full_unstemmed |
Diagnosis of hepatitis C virus in Brazilian blood donors using a reverse transcriptase nested polymerase chain reaction: comparison with enzyme immunoassay and recombinant protein immunoblot assay |
title_sort |
diagnosis of hepatitis c virus in brazilian blood donors using a reverse transcriptase nested polymerase chain reaction: comparison with enzyme immunoassay and recombinant protein immunoblot assay |
publisher |
Universidade de São Paulo (USP) |
publishDate |
2000 |
url |
https://doaj.org/article/3f461127a1c44c128de91a71e95af3cf |
long_lat |
ENVELOPE(7.755,7.755,63.024,63.024) |
geographic |
Arctic Eia |
geographic_facet |
Arctic Eia |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Revista do Instituto de Medicina Tropical de São Paulo, Vol 42, Iss 5, Pp 263-267 (2000) |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0036-46652000000500005&tlng=en https://doaj.org/toc/1678-9946 1678-9946 https://doaj.org/article/3f461127a1c44c128de91a71e95af3cf |
_version_ |
1809897967482568704 |