Quality of Sterile Male Tsetse after Long Distance Transport as Chilled, Irradiated Pupae.
BACKGROUND:Tsetse flies transmit trypanosomes that cause human and African animal trypanosomosis, a debilitating disease of humans (sleeping sickness) and livestock (nagana). An area-wide integrated pest management campaign against Glossina palpalis gambiensis has been implemented in Senegal since 2...
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ftdoajarticles:oai:doaj.org/article:3f1b4e5d589840c3b8062e7ecb49cac2 2023-05-15T15:11:44+02:00 Quality of Sterile Male Tsetse after Long Distance Transport as Chilled, Irradiated Pupae. Momar Talla Seck Soumaïla Pagabeleguem Mireille D Bassene Assane Gueye Fall Thérèse A R Diouf Baba Sall Marc J B Vreysen Jean-Baptiste Rayaissé Peter Takac Issa Sidibé Andrew G Parker Gratian N Mutika Jérémy Bouyer Geoffrey Gimonneau 2015-11-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0004229 https://doaj.org/article/3f1b4e5d589840c3b8062e7ecb49cac2 EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC4642948?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0004229 https://doaj.org/article/3f1b4e5d589840c3b8062e7ecb49cac2 PLoS Neglected Tropical Diseases, Vol 9, Iss 11, p e0004229 (2015) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2015 ftdoajarticles https://doi.org/10.1371/journal.pntd.0004229 2022-12-30T20:49:54Z BACKGROUND:Tsetse flies transmit trypanosomes that cause human and African animal trypanosomosis, a debilitating disease of humans (sleeping sickness) and livestock (nagana). An area-wide integrated pest management campaign against Glossina palpalis gambiensis has been implemented in Senegal since 2010 that includes a sterile insect technique (SIT) component. The SIT can only be successful when the sterile males that are destined for release have a flight ability, survival and competitiveness that are as close as possible to that of their wild male counterparts. METHODOLOGY/PRINCIPAL FINDINGS:Tests were developed to assess the quality of G. p. gambiensis males that emerged from pupae that were produced and irradiated in Burkina Faso and Slovakia (irradiation done in Seibersdorf, Austria) and transported weekly under chilled conditions to Dakar, Senegal. For each consignment a sample of 50 pupae was used for a quality control test (QC group). To assess flight ability, the pupae were put in a cylinder filtering emerged flies that were able to escape the cylinder. The survival of these flyers was thereafter monitored under stress conditions (without feeding). Remaining pupae were emerged and released in the target area of the eradication programme (RF group). The following parameter values were obtained for the QC flies: average emergence rate more than 69%, median survival of 6 days, and average flight ability of more than 35%. The quality protocol was a good proxy of fly quality, explaining a large part of the variances of the examined parameters. CONCLUSIONS/SIGNIFICANCE:The quality protocol described here will allow the accurate monitoring of the quality of shipped sterile male tsetse used in operational eradication programmes in the framework of the Pan-African Tsetse and Trypanosomosis Eradication Campaign. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 9 11 e0004229 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
spellingShingle |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Momar Talla Seck Soumaïla Pagabeleguem Mireille D Bassene Assane Gueye Fall Thérèse A R Diouf Baba Sall Marc J B Vreysen Jean-Baptiste Rayaissé Peter Takac Issa Sidibé Andrew G Parker Gratian N Mutika Jérémy Bouyer Geoffrey Gimonneau Quality of Sterile Male Tsetse after Long Distance Transport as Chilled, Irradiated Pupae. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
BACKGROUND:Tsetse flies transmit trypanosomes that cause human and African animal trypanosomosis, a debilitating disease of humans (sleeping sickness) and livestock (nagana). An area-wide integrated pest management campaign against Glossina palpalis gambiensis has been implemented in Senegal since 2010 that includes a sterile insect technique (SIT) component. The SIT can only be successful when the sterile males that are destined for release have a flight ability, survival and competitiveness that are as close as possible to that of their wild male counterparts. METHODOLOGY/PRINCIPAL FINDINGS:Tests were developed to assess the quality of G. p. gambiensis males that emerged from pupae that were produced and irradiated in Burkina Faso and Slovakia (irradiation done in Seibersdorf, Austria) and transported weekly under chilled conditions to Dakar, Senegal. For each consignment a sample of 50 pupae was used for a quality control test (QC group). To assess flight ability, the pupae were put in a cylinder filtering emerged flies that were able to escape the cylinder. The survival of these flyers was thereafter monitored under stress conditions (without feeding). Remaining pupae were emerged and released in the target area of the eradication programme (RF group). The following parameter values were obtained for the QC flies: average emergence rate more than 69%, median survival of 6 days, and average flight ability of more than 35%. The quality protocol was a good proxy of fly quality, explaining a large part of the variances of the examined parameters. CONCLUSIONS/SIGNIFICANCE:The quality protocol described here will allow the accurate monitoring of the quality of shipped sterile male tsetse used in operational eradication programmes in the framework of the Pan-African Tsetse and Trypanosomosis Eradication Campaign. |
format |
Article in Journal/Newspaper |
author |
Momar Talla Seck Soumaïla Pagabeleguem Mireille D Bassene Assane Gueye Fall Thérèse A R Diouf Baba Sall Marc J B Vreysen Jean-Baptiste Rayaissé Peter Takac Issa Sidibé Andrew G Parker Gratian N Mutika Jérémy Bouyer Geoffrey Gimonneau |
author_facet |
Momar Talla Seck Soumaïla Pagabeleguem Mireille D Bassene Assane Gueye Fall Thérèse A R Diouf Baba Sall Marc J B Vreysen Jean-Baptiste Rayaissé Peter Takac Issa Sidibé Andrew G Parker Gratian N Mutika Jérémy Bouyer Geoffrey Gimonneau |
author_sort |
Momar Talla Seck |
title |
Quality of Sterile Male Tsetse after Long Distance Transport as Chilled, Irradiated Pupae. |
title_short |
Quality of Sterile Male Tsetse after Long Distance Transport as Chilled, Irradiated Pupae. |
title_full |
Quality of Sterile Male Tsetse after Long Distance Transport as Chilled, Irradiated Pupae. |
title_fullStr |
Quality of Sterile Male Tsetse after Long Distance Transport as Chilled, Irradiated Pupae. |
title_full_unstemmed |
Quality of Sterile Male Tsetse after Long Distance Transport as Chilled, Irradiated Pupae. |
title_sort |
quality of sterile male tsetse after long distance transport as chilled, irradiated pupae. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2015 |
url |
https://doi.org/10.1371/journal.pntd.0004229 https://doaj.org/article/3f1b4e5d589840c3b8062e7ecb49cac2 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 9, Iss 11, p e0004229 (2015) |
op_relation |
http://europepmc.org/articles/PMC4642948?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0004229 https://doaj.org/article/3f1b4e5d589840c3b8062e7ecb49cac2 |
op_doi |
https://doi.org/10.1371/journal.pntd.0004229 |
container_title |
PLOS Neglected Tropical Diseases |
container_volume |
9 |
container_issue |
11 |
container_start_page |
e0004229 |
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1766342539634278400 |