Diversity and potential activity of methanotrophs in high methane-emitting permafrost thaw ponds.
Lakes and ponds derived from thawing permafrost are strong emitters of carbon dioxide and methane to the atmosphere, but little is known about the methane oxidation processes in these waters. Here we investigated the distribution and potential activity of aerobic methanotrophic bacteria in thaw pond...
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ftdoajarticles:oai:doaj.org/article:3ef99aa033034dd6a063de550866659f 2023-05-15T17:56:55+02:00 Diversity and potential activity of methanotrophs in high methane-emitting permafrost thaw ponds. Sophie Crevecoeur Warwick F Vincent Jérôme Comte Alex Matveev Connie Lovejoy 2017-01-01T00:00:00Z https://doi.org/10.1371/journal.pone.0188223 https://doaj.org/article/3ef99aa033034dd6a063de550866659f EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC5705078?pdf=render https://doaj.org/toc/1932-6203 1932-6203 doi:10.1371/journal.pone.0188223 https://doaj.org/article/3ef99aa033034dd6a063de550866659f PLoS ONE, Vol 12, Iss 11, p e0188223 (2017) Medicine R Science Q article 2017 ftdoajarticles https://doi.org/10.1371/journal.pone.0188223 2022-12-31T02:13:22Z Lakes and ponds derived from thawing permafrost are strong emitters of carbon dioxide and methane to the atmosphere, but little is known about the methane oxidation processes in these waters. Here we investigated the distribution and potential activity of aerobic methanotrophic bacteria in thaw ponds in two types of eroding permafrost landscapes in subarctic Québec: peatlands and mineral soils. We hypothesized that methanotrophic community composition and potential activity differ regionally as a function of the landscape type and permafrost degradation stage, and locally as a function of depth-dependent oxygen conditions. Our analysis of pmoA transcripts by Illumina amplicon sequencing and quantitative PCR showed that the communities were composed of diverse and potentially active lineages. Type I methanotrophs, particularly Methylobacter, dominated all communities, however there was a clear taxonomic separation between the two landscape types, consistent with environmental control of community structure. In contrast, methanotrophic potential activity, measured by pmoA transcript concentrations, did not vary with landscape type, but correlated with conductivity, phosphorus and total suspended solids. Methanotrophic potential activity was also detected in low-oxygen bottom waters, where it was inversely correlated with methane concentrations, suggesting methane depletion by methanotrophs. Methanotrophs were present and potentially active throughout the water column regardless of oxygen concentration, and may therefore be resilient to future mixing and oxygenation regimes in the warming subarctic. Article in Journal/Newspaper permafrost Subarctic Directory of Open Access Journals: DOAJ Articles PLOS ONE 12 11 e0188223 |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
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English |
topic |
Medicine R Science Q |
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Medicine R Science Q Sophie Crevecoeur Warwick F Vincent Jérôme Comte Alex Matveev Connie Lovejoy Diversity and potential activity of methanotrophs in high methane-emitting permafrost thaw ponds. |
topic_facet |
Medicine R Science Q |
description |
Lakes and ponds derived from thawing permafrost are strong emitters of carbon dioxide and methane to the atmosphere, but little is known about the methane oxidation processes in these waters. Here we investigated the distribution and potential activity of aerobic methanotrophic bacteria in thaw ponds in two types of eroding permafrost landscapes in subarctic Québec: peatlands and mineral soils. We hypothesized that methanotrophic community composition and potential activity differ regionally as a function of the landscape type and permafrost degradation stage, and locally as a function of depth-dependent oxygen conditions. Our analysis of pmoA transcripts by Illumina amplicon sequencing and quantitative PCR showed that the communities were composed of diverse and potentially active lineages. Type I methanotrophs, particularly Methylobacter, dominated all communities, however there was a clear taxonomic separation between the two landscape types, consistent with environmental control of community structure. In contrast, methanotrophic potential activity, measured by pmoA transcript concentrations, did not vary with landscape type, but correlated with conductivity, phosphorus and total suspended solids. Methanotrophic potential activity was also detected in low-oxygen bottom waters, where it was inversely correlated with methane concentrations, suggesting methane depletion by methanotrophs. Methanotrophs were present and potentially active throughout the water column regardless of oxygen concentration, and may therefore be resilient to future mixing and oxygenation regimes in the warming subarctic. |
format |
Article in Journal/Newspaper |
author |
Sophie Crevecoeur Warwick F Vincent Jérôme Comte Alex Matveev Connie Lovejoy |
author_facet |
Sophie Crevecoeur Warwick F Vincent Jérôme Comte Alex Matveev Connie Lovejoy |
author_sort |
Sophie Crevecoeur |
title |
Diversity and potential activity of methanotrophs in high methane-emitting permafrost thaw ponds. |
title_short |
Diversity and potential activity of methanotrophs in high methane-emitting permafrost thaw ponds. |
title_full |
Diversity and potential activity of methanotrophs in high methane-emitting permafrost thaw ponds. |
title_fullStr |
Diversity and potential activity of methanotrophs in high methane-emitting permafrost thaw ponds. |
title_full_unstemmed |
Diversity and potential activity of methanotrophs in high methane-emitting permafrost thaw ponds. |
title_sort |
diversity and potential activity of methanotrophs in high methane-emitting permafrost thaw ponds. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2017 |
url |
https://doi.org/10.1371/journal.pone.0188223 https://doaj.org/article/3ef99aa033034dd6a063de550866659f |
genre |
permafrost Subarctic |
genre_facet |
permafrost Subarctic |
op_source |
PLoS ONE, Vol 12, Iss 11, p e0188223 (2017) |
op_relation |
http://europepmc.org/articles/PMC5705078?pdf=render https://doaj.org/toc/1932-6203 1932-6203 doi:10.1371/journal.pone.0188223 https://doaj.org/article/3ef99aa033034dd6a063de550866659f |
op_doi |
https://doi.org/10.1371/journal.pone.0188223 |
container_title |
PLOS ONE |
container_volume |
12 |
container_issue |
11 |
container_start_page |
e0188223 |
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1766165237533245440 |