Osteopontin impairs host defense during established gram-negative sepsis caused by Burkholderia pseudomallei (melioidosis).
Melioidosis, caused by infection with Burkholderia (B.) pseudomallei, is a severe illness that is endemic in Southeast Asia. Osteopontin (OPN) is a phosphorylated glycoprotein that is involved in several immune responses including induction of T-helper 1 cytokines and recruitment of inflammatory cel...
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ftdoajarticles:oai:doaj.org/article:3ed62197ca3b40a0a084942b23fcba40 2023-05-15T15:07:25+02:00 Osteopontin impairs host defense during established gram-negative sepsis caused by Burkholderia pseudomallei (melioidosis). Gerritje J W van der Windt W Joost Wiersinga Catharina W Wieland Ivo C S I Tjia Nicholas P Day Sharon J Peacock Sandrine Florquin Tom van der Poll 2010-08-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0000806 https://doaj.org/article/3ed62197ca3b40a0a084942b23fcba40 EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC2930856?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0000806 https://doaj.org/article/3ed62197ca3b40a0a084942b23fcba40 PLoS Neglected Tropical Diseases, Vol 4, Iss 8 (2010) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2010 ftdoajarticles https://doi.org/10.1371/journal.pntd.0000806 2022-12-31T11:54:57Z Melioidosis, caused by infection with Burkholderia (B.) pseudomallei, is a severe illness that is endemic in Southeast Asia. Osteopontin (OPN) is a phosphorylated glycoprotein that is involved in several immune responses including induction of T-helper 1 cytokines and recruitment of inflammatory cells.OPN levels were determined in plasma from 33 melioidosis patients and 31 healthy controls, and in wild-type (WT) mice intranasally infected with B. pseudomallei. OPN function was studied in experimental murine melioidosis using WT and OPN knockout (KO) mice. Plasma OPN levels were elevated in patients with severe melioidosis, even more so in patients who went on to die. In patients who recovered plasma OPN concentrations had decreased after treatment. In experimental melioidosis in mice plasma and pulmonary OPN levels were also increased. Whereas WT and OPN KO mice were indistinguishable during the first 24 hours after infection, after 72 hours OPN KO mice demonstrated reduced bacterial numbers in their lungs, diminished pulmonary tissue injury, especially due to less necrosis, and decreased neutrophil infiltration. Moreover, OPN KO mice displayed a delayed mortality as compared to WT mice. OPN deficiency did not influence the induction of proinflammatory cytokines.These data suggest that sustained production of OPN impairs host defense during established septic melioidosis. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLoS Neglected Tropical Diseases 4 8 e806 |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
spellingShingle |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Gerritje J W van der Windt W Joost Wiersinga Catharina W Wieland Ivo C S I Tjia Nicholas P Day Sharon J Peacock Sandrine Florquin Tom van der Poll Osteopontin impairs host defense during established gram-negative sepsis caused by Burkholderia pseudomallei (melioidosis). |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Melioidosis, caused by infection with Burkholderia (B.) pseudomallei, is a severe illness that is endemic in Southeast Asia. Osteopontin (OPN) is a phosphorylated glycoprotein that is involved in several immune responses including induction of T-helper 1 cytokines and recruitment of inflammatory cells.OPN levels were determined in plasma from 33 melioidosis patients and 31 healthy controls, and in wild-type (WT) mice intranasally infected with B. pseudomallei. OPN function was studied in experimental murine melioidosis using WT and OPN knockout (KO) mice. Plasma OPN levels were elevated in patients with severe melioidosis, even more so in patients who went on to die. In patients who recovered plasma OPN concentrations had decreased after treatment. In experimental melioidosis in mice plasma and pulmonary OPN levels were also increased. Whereas WT and OPN KO mice were indistinguishable during the first 24 hours after infection, after 72 hours OPN KO mice demonstrated reduced bacterial numbers in their lungs, diminished pulmonary tissue injury, especially due to less necrosis, and decreased neutrophil infiltration. Moreover, OPN KO mice displayed a delayed mortality as compared to WT mice. OPN deficiency did not influence the induction of proinflammatory cytokines.These data suggest that sustained production of OPN impairs host defense during established septic melioidosis. |
format |
Article in Journal/Newspaper |
author |
Gerritje J W van der Windt W Joost Wiersinga Catharina W Wieland Ivo C S I Tjia Nicholas P Day Sharon J Peacock Sandrine Florquin Tom van der Poll |
author_facet |
Gerritje J W van der Windt W Joost Wiersinga Catharina W Wieland Ivo C S I Tjia Nicholas P Day Sharon J Peacock Sandrine Florquin Tom van der Poll |
author_sort |
Gerritje J W van der Windt |
title |
Osteopontin impairs host defense during established gram-negative sepsis caused by Burkholderia pseudomallei (melioidosis). |
title_short |
Osteopontin impairs host defense during established gram-negative sepsis caused by Burkholderia pseudomallei (melioidosis). |
title_full |
Osteopontin impairs host defense during established gram-negative sepsis caused by Burkholderia pseudomallei (melioidosis). |
title_fullStr |
Osteopontin impairs host defense during established gram-negative sepsis caused by Burkholderia pseudomallei (melioidosis). |
title_full_unstemmed |
Osteopontin impairs host defense during established gram-negative sepsis caused by Burkholderia pseudomallei (melioidosis). |
title_sort |
osteopontin impairs host defense during established gram-negative sepsis caused by burkholderia pseudomallei (melioidosis). |
publisher |
Public Library of Science (PLoS) |
publishDate |
2010 |
url |
https://doi.org/10.1371/journal.pntd.0000806 https://doaj.org/article/3ed62197ca3b40a0a084942b23fcba40 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 4, Iss 8 (2010) |
op_relation |
http://europepmc.org/articles/PMC2930856?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0000806 https://doaj.org/article/3ed62197ca3b40a0a084942b23fcba40 |
op_doi |
https://doi.org/10.1371/journal.pntd.0000806 |
container_title |
PLoS Neglected Tropical Diseases |
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4 |
container_issue |
8 |
container_start_page |
e806 |
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