Diagnostic performance of a single and duplicate Kato-Katz, Mini-FLOTAC, FECPAKG2 and qPCR for the detection and quantification of soil-transmitted helminths in three endemic countries.
Background Because the success of deworming programs targeting soil-transmitted helminths (STHs) is evaluated through the periodically assessment of prevalence and infection intensities, the use of the correct diagnostic method is of utmost importance. The STH community has recently published for ea...
Published in: | PLOS Neglected Tropical Diseases |
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ftdoajarticles:oai:doaj.org/article:3e919f760a36453d968ed82d58ecfb49 2023-05-15T15:17:12+02:00 Diagnostic performance of a single and duplicate Kato-Katz, Mini-FLOTAC, FECPAKG2 and qPCR for the detection and quantification of soil-transmitted helminths in three endemic countries. Piet Cools Johnny Vlaminck Marco Albonico Shaali Ame Mio Ayana Barrios Perez José Antonio Giuseppe Cringoli Daniel Dana Jennifer Keiser Maria P Maurelli Catalina Maya Leonardo F Matoso Antonio Montresor Zeleke Mekonnen Greg Mirams Rodrigo Corrêa-Oliveira Simone A Pinto Laura Rinaldi Somphou Sayasone Eurion Thomas Jaco J Verweij Jozef Vercruysse Bruno Levecke 2019-08-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0007446 https://doaj.org/article/3e919f760a36453d968ed82d58ecfb49 EN eng Public Library of Science (PLoS) https://doi.org/10.1371/journal.pntd.0007446 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0007446 https://doaj.org/article/3e919f760a36453d968ed82d58ecfb49 PLoS Neglected Tropical Diseases, Vol 13, Iss 8, p e0007446 (2019) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2019 ftdoajarticles https://doi.org/10.1371/journal.pntd.0007446 2022-12-31T04:02:48Z Background Because the success of deworming programs targeting soil-transmitted helminths (STHs) is evaluated through the periodically assessment of prevalence and infection intensities, the use of the correct diagnostic method is of utmost importance. The STH community has recently published for each phase of a deworming program the minimal criteria that a potential diagnostic method needs to meet, the so-called target product profiles (TPPs). Methodology We compared the diagnostic performance of a single Kato-Katz (reference method) with that of other microscopy-based methods (duplicate Kato-Katz, Mini-FLOTAC and FECPAKG2) and one DNA-based method (qPCR) for the detection and quantification of STH infections in three drug efficacy trials in Ethiopia, Lao PDR, and Tanzania. Furthermore, we evaluated a selection of minimal diagnostic criteria of the TPPs. Principal findings All diagnostic methods showed a clinical sensitivity of ≥90% for all STH infections of moderate-to-heavy intensities. For infections of very low intensity, only qPCR resulted in a sensitivity that was superior to a single Kato-Katz for all STHs. Compared to the reference method, both Mini-FLOTAC and FECPAKG2 resulted in significantly lower fecal egg counts for some STHs, leading to a substantial underestimation of the infection intensity. For qPCR, there was a positive significant correlation between the egg counts of a single Kato-Katz and the DNA concentration. Conclusions/significance Our results indicate that the diagnostic performance of a single Kato-Katz is underestimated by the community and that diagnostic specific thresholds to classify intensity of infection are warranted for Mini-FLOTAC, FECPAKG2 and qPCR. When we strictly apply the TPPs, Kato-Katz is the only microscopy-based method that meets the minimal diagnostic criteria for application in the planning, monitoring and evaluation phase of an STH program. qPCR is the only method that could be considered in the phase that aims to seek confirmation for cessation of program. Trial ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 13 8 e0007446 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
spellingShingle |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Piet Cools Johnny Vlaminck Marco Albonico Shaali Ame Mio Ayana Barrios Perez José Antonio Giuseppe Cringoli Daniel Dana Jennifer Keiser Maria P Maurelli Catalina Maya Leonardo F Matoso Antonio Montresor Zeleke Mekonnen Greg Mirams Rodrigo Corrêa-Oliveira Simone A Pinto Laura Rinaldi Somphou Sayasone Eurion Thomas Jaco J Verweij Jozef Vercruysse Bruno Levecke Diagnostic performance of a single and duplicate Kato-Katz, Mini-FLOTAC, FECPAKG2 and qPCR for the detection and quantification of soil-transmitted helminths in three endemic countries. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Background Because the success of deworming programs targeting soil-transmitted helminths (STHs) is evaluated through the periodically assessment of prevalence and infection intensities, the use of the correct diagnostic method is of utmost importance. The STH community has recently published for each phase of a deworming program the minimal criteria that a potential diagnostic method needs to meet, the so-called target product profiles (TPPs). Methodology We compared the diagnostic performance of a single Kato-Katz (reference method) with that of other microscopy-based methods (duplicate Kato-Katz, Mini-FLOTAC and FECPAKG2) and one DNA-based method (qPCR) for the detection and quantification of STH infections in three drug efficacy trials in Ethiopia, Lao PDR, and Tanzania. Furthermore, we evaluated a selection of minimal diagnostic criteria of the TPPs. Principal findings All diagnostic methods showed a clinical sensitivity of ≥90% for all STH infections of moderate-to-heavy intensities. For infections of very low intensity, only qPCR resulted in a sensitivity that was superior to a single Kato-Katz for all STHs. Compared to the reference method, both Mini-FLOTAC and FECPAKG2 resulted in significantly lower fecal egg counts for some STHs, leading to a substantial underestimation of the infection intensity. For qPCR, there was a positive significant correlation between the egg counts of a single Kato-Katz and the DNA concentration. Conclusions/significance Our results indicate that the diagnostic performance of a single Kato-Katz is underestimated by the community and that diagnostic specific thresholds to classify intensity of infection are warranted for Mini-FLOTAC, FECPAKG2 and qPCR. When we strictly apply the TPPs, Kato-Katz is the only microscopy-based method that meets the minimal diagnostic criteria for application in the planning, monitoring and evaluation phase of an STH program. qPCR is the only method that could be considered in the phase that aims to seek confirmation for cessation of program. Trial ... |
format |
Article in Journal/Newspaper |
author |
Piet Cools Johnny Vlaminck Marco Albonico Shaali Ame Mio Ayana Barrios Perez José Antonio Giuseppe Cringoli Daniel Dana Jennifer Keiser Maria P Maurelli Catalina Maya Leonardo F Matoso Antonio Montresor Zeleke Mekonnen Greg Mirams Rodrigo Corrêa-Oliveira Simone A Pinto Laura Rinaldi Somphou Sayasone Eurion Thomas Jaco J Verweij Jozef Vercruysse Bruno Levecke |
author_facet |
Piet Cools Johnny Vlaminck Marco Albonico Shaali Ame Mio Ayana Barrios Perez José Antonio Giuseppe Cringoli Daniel Dana Jennifer Keiser Maria P Maurelli Catalina Maya Leonardo F Matoso Antonio Montresor Zeleke Mekonnen Greg Mirams Rodrigo Corrêa-Oliveira Simone A Pinto Laura Rinaldi Somphou Sayasone Eurion Thomas Jaco J Verweij Jozef Vercruysse Bruno Levecke |
author_sort |
Piet Cools |
title |
Diagnostic performance of a single and duplicate Kato-Katz, Mini-FLOTAC, FECPAKG2 and qPCR for the detection and quantification of soil-transmitted helminths in three endemic countries. |
title_short |
Diagnostic performance of a single and duplicate Kato-Katz, Mini-FLOTAC, FECPAKG2 and qPCR for the detection and quantification of soil-transmitted helminths in three endemic countries. |
title_full |
Diagnostic performance of a single and duplicate Kato-Katz, Mini-FLOTAC, FECPAKG2 and qPCR for the detection and quantification of soil-transmitted helminths in three endemic countries. |
title_fullStr |
Diagnostic performance of a single and duplicate Kato-Katz, Mini-FLOTAC, FECPAKG2 and qPCR for the detection and quantification of soil-transmitted helminths in three endemic countries. |
title_full_unstemmed |
Diagnostic performance of a single and duplicate Kato-Katz, Mini-FLOTAC, FECPAKG2 and qPCR for the detection and quantification of soil-transmitted helminths in three endemic countries. |
title_sort |
diagnostic performance of a single and duplicate kato-katz, mini-flotac, fecpakg2 and qpcr for the detection and quantification of soil-transmitted helminths in three endemic countries. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2019 |
url |
https://doi.org/10.1371/journal.pntd.0007446 https://doaj.org/article/3e919f760a36453d968ed82d58ecfb49 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 13, Iss 8, p e0007446 (2019) |
op_relation |
https://doi.org/10.1371/journal.pntd.0007446 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0007446 https://doaj.org/article/3e919f760a36453d968ed82d58ecfb49 |
op_doi |
https://doi.org/10.1371/journal.pntd.0007446 |
container_title |
PLOS Neglected Tropical Diseases |
container_volume |
13 |
container_issue |
8 |
container_start_page |
e0007446 |
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