Defining Brugia malayi and Wolbachia symbiosis by stage-specific dual RNA-seq.
BACKGROUND:Filarial nematodes currently infect up to 54 million people worldwide, with millions more at risk for infection, representing the leading cause of disability in the developing world. Brugia malayi is one of the causative agents of lymphatic filariasis and remains the only human filarial p...
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ftdoajarticles:oai:doaj.org/article:3e53b1bd97384a11abeb99892dad15d8 2023-05-15T15:10:35+02:00 Defining Brugia malayi and Wolbachia symbiosis by stage-specific dual RNA-seq. Alexandra Grote Denis Voronin Tao Ding Alan Twaddle Thomas R Unnasch Sara Lustigman Elodie Ghedin 2017-03-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0005357 https://doaj.org/article/3e53b1bd97384a11abeb99892dad15d8 EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC5373514?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0005357 https://doaj.org/article/3e53b1bd97384a11abeb99892dad15d8 PLoS Neglected Tropical Diseases, Vol 11, Iss 3, p e0005357 (2017) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2017 ftdoajarticles https://doi.org/10.1371/journal.pntd.0005357 2022-12-31T03:01:41Z BACKGROUND:Filarial nematodes currently infect up to 54 million people worldwide, with millions more at risk for infection, representing the leading cause of disability in the developing world. Brugia malayi is one of the causative agents of lymphatic filariasis and remains the only human filarial parasite that can be maintained in small laboratory animals. Many filarial nematode species, including B. malayi, carry an obligate endosymbiont, the alpha-proteobacteria Wolbachia, which can be eliminated through antibiotic treatment. Elimination of the endosymbiont interferes with development, reproduction, and survival of the worms within the mamalian host, a clear indicator that the Wolbachia are crucial for survival of the parasite. Little is understood about the mechanism underlying this symbiosis. METHODOLOGY/ PRINCIPLE FINDINGS:To better understand the molecular interplay between these two organisms we profiled the transcriptomes of B. malayi and Wolbachia by dual RNA-seq across the life cycle of the parasite. This helped identify functional pathways involved in this essential symbiotic relationship provided by the co-expression of nematode and bacterial genes. We have identified significant stage-specific and gender-specific differential expression in Wolbachia during the nematode's development. For example, during female worm development we find that Wolbachia upregulate genes involved in ATP production and purine biosynthesis, as well as genes involved in the oxidative stress response. CONCLUSIONS/ SIGNIFICANCE:This global transcriptional analysis has highlighted specific pathways to which both Wolbachia and B. malayi contribute concurrently over the life cycle of the parasite, paving the way for the development of novel intervention strategies. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 11 3 e0005357 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Alexandra Grote Denis Voronin Tao Ding Alan Twaddle Thomas R Unnasch Sara Lustigman Elodie Ghedin Defining Brugia malayi and Wolbachia symbiosis by stage-specific dual RNA-seq. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
BACKGROUND:Filarial nematodes currently infect up to 54 million people worldwide, with millions more at risk for infection, representing the leading cause of disability in the developing world. Brugia malayi is one of the causative agents of lymphatic filariasis and remains the only human filarial parasite that can be maintained in small laboratory animals. Many filarial nematode species, including B. malayi, carry an obligate endosymbiont, the alpha-proteobacteria Wolbachia, which can be eliminated through antibiotic treatment. Elimination of the endosymbiont interferes with development, reproduction, and survival of the worms within the mamalian host, a clear indicator that the Wolbachia are crucial for survival of the parasite. Little is understood about the mechanism underlying this symbiosis. METHODOLOGY/ PRINCIPLE FINDINGS:To better understand the molecular interplay between these two organisms we profiled the transcriptomes of B. malayi and Wolbachia by dual RNA-seq across the life cycle of the parasite. This helped identify functional pathways involved in this essential symbiotic relationship provided by the co-expression of nematode and bacterial genes. We have identified significant stage-specific and gender-specific differential expression in Wolbachia during the nematode's development. For example, during female worm development we find that Wolbachia upregulate genes involved in ATP production and purine biosynthesis, as well as genes involved in the oxidative stress response. CONCLUSIONS/ SIGNIFICANCE:This global transcriptional analysis has highlighted specific pathways to which both Wolbachia and B. malayi contribute concurrently over the life cycle of the parasite, paving the way for the development of novel intervention strategies. |
format |
Article in Journal/Newspaper |
author |
Alexandra Grote Denis Voronin Tao Ding Alan Twaddle Thomas R Unnasch Sara Lustigman Elodie Ghedin |
author_facet |
Alexandra Grote Denis Voronin Tao Ding Alan Twaddle Thomas R Unnasch Sara Lustigman Elodie Ghedin |
author_sort |
Alexandra Grote |
title |
Defining Brugia malayi and Wolbachia symbiosis by stage-specific dual RNA-seq. |
title_short |
Defining Brugia malayi and Wolbachia symbiosis by stage-specific dual RNA-seq. |
title_full |
Defining Brugia malayi and Wolbachia symbiosis by stage-specific dual RNA-seq. |
title_fullStr |
Defining Brugia malayi and Wolbachia symbiosis by stage-specific dual RNA-seq. |
title_full_unstemmed |
Defining Brugia malayi and Wolbachia symbiosis by stage-specific dual RNA-seq. |
title_sort |
defining brugia malayi and wolbachia symbiosis by stage-specific dual rna-seq. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2017 |
url |
https://doi.org/10.1371/journal.pntd.0005357 https://doaj.org/article/3e53b1bd97384a11abeb99892dad15d8 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 11, Iss 3, p e0005357 (2017) |
op_relation |
http://europepmc.org/articles/PMC5373514?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0005357 https://doaj.org/article/3e53b1bd97384a11abeb99892dad15d8 |
op_doi |
https://doi.org/10.1371/journal.pntd.0005357 |
container_title |
PLOS Neglected Tropical Diseases |
container_volume |
11 |
container_issue |
3 |
container_start_page |
e0005357 |
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1766341585145954304 |