APPLICABILITY OF kDNA-PCR FOR ROUTINE DIAGNOSIS OF AMERICAN TEGUMENTARY LEISHMANIASIS IN A TERTIARY REFERENCE HOSPITAL
SUMMARY This study evaluated the applicability of kDNA-PCR as a prospective routine diagnosis method for American tegumentary leishmaniasis (ATL) in patients from the Instituto de Infectologia Emílio Ribas (IIER), a reference center for infectious diseases in São Paulo - SP, Brazil. The kDNA-PCR met...
Published in: | Revista do Instituto de Medicina Tropical de São Paulo |
---|---|
Main Authors: | , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Universidade de São Paulo (USP)
2013
|
Subjects: | |
Online Access: | https://doi.org/10.1590/S0036-46652013000600004 https://doaj.org/article/3e4d6edd2f9c40f39ee4fac52ef7bf6d |
id |
ftdoajarticles:oai:doaj.org/article:3e4d6edd2f9c40f39ee4fac52ef7bf6d |
---|---|
record_format |
openpolar |
spelling |
ftdoajarticles:oai:doaj.org/article:3e4d6edd2f9c40f39ee4fac52ef7bf6d 2024-09-09T19:25:33+00:00 APPLICABILITY OF kDNA-PCR FOR ROUTINE DIAGNOSIS OF AMERICAN TEGUMENTARY LEISHMANIASIS IN A TERTIARY REFERENCE HOSPITAL Marcela M. Satow Edite H. Yamashiro-Kanashiro Mussya C. Rocha Luiza K. Oyafuso Rita C. Soler Paulo C. Cotrim Jose Angelo L. Lindoso 2013-12-01T00:00:00Z https://doi.org/10.1590/S0036-46652013000600004 https://doaj.org/article/3e4d6edd2f9c40f39ee4fac52ef7bf6d EN eng Universidade de São Paulo (USP) http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0036-46652013000600393&lng=en&tlng=en https://doaj.org/toc/1678-9946 1678-9946 doi:10.1590/S0036-46652013000600004 https://doaj.org/article/3e4d6edd2f9c40f39ee4fac52ef7bf6d Revista do Instituto de Medicina Tropical de São Paulo, Vol 55, Iss 6, Pp 393-399 (2013) American tegumentary leishmaniasis Diagnostic kDNA-PCR Leishmania (Viannia) braziliensis Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 2013 ftdoajarticles https://doi.org/10.1590/S0036-46652013000600004 2024-08-05T17:49:30Z SUMMARY This study evaluated the applicability of kDNA-PCR as a prospective routine diagnosis method for American tegumentary leishmaniasis (ATL) in patients from the Instituto de Infectologia Emílio Ribas (IIER), a reference center for infectious diseases in São Paulo - SP, Brazil. The kDNA-PCR method detected Leishmania DNA in 87.5% (112/128) of the clinically suspected ATL patients, while the traditional methods demonstrated the following percentages of positivity: 62.8% (49/78) for the Montenegro skin test, 61.8% (47/76) for direct investigation, and 19.3% (22/114) for in vitro culture. The molecular method was able to confirm the disease in samples considered negative or inconclusive by traditional laboratory methods, contributing to the final clinical diagnosis and therapy of ATL in this hospital. Thus, we strongly recommend the inclusion of kDNA-PCR amplification as an alternative diagnostic method for ATL, suggesting a new algorithm routine to be followed to help the diagnosis and treatment of ATL in IIER. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Revista do Instituto de Medicina Tropical de São Paulo 55 6 393 399 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
American tegumentary leishmaniasis Diagnostic kDNA-PCR Leishmania (Viannia) braziliensis Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
spellingShingle |
American tegumentary leishmaniasis Diagnostic kDNA-PCR Leishmania (Viannia) braziliensis Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 Marcela M. Satow Edite H. Yamashiro-Kanashiro Mussya C. Rocha Luiza K. Oyafuso Rita C. Soler Paulo C. Cotrim Jose Angelo L. Lindoso APPLICABILITY OF kDNA-PCR FOR ROUTINE DIAGNOSIS OF AMERICAN TEGUMENTARY LEISHMANIASIS IN A TERTIARY REFERENCE HOSPITAL |
topic_facet |
American tegumentary leishmaniasis Diagnostic kDNA-PCR Leishmania (Viannia) braziliensis Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
SUMMARY This study evaluated the applicability of kDNA-PCR as a prospective routine diagnosis method for American tegumentary leishmaniasis (ATL) in patients from the Instituto de Infectologia Emílio Ribas (IIER), a reference center for infectious diseases in São Paulo - SP, Brazil. The kDNA-PCR method detected Leishmania DNA in 87.5% (112/128) of the clinically suspected ATL patients, while the traditional methods demonstrated the following percentages of positivity: 62.8% (49/78) for the Montenegro skin test, 61.8% (47/76) for direct investigation, and 19.3% (22/114) for in vitro culture. The molecular method was able to confirm the disease in samples considered negative or inconclusive by traditional laboratory methods, contributing to the final clinical diagnosis and therapy of ATL in this hospital. Thus, we strongly recommend the inclusion of kDNA-PCR amplification as an alternative diagnostic method for ATL, suggesting a new algorithm routine to be followed to help the diagnosis and treatment of ATL in IIER. |
format |
Article in Journal/Newspaper |
author |
Marcela M. Satow Edite H. Yamashiro-Kanashiro Mussya C. Rocha Luiza K. Oyafuso Rita C. Soler Paulo C. Cotrim Jose Angelo L. Lindoso |
author_facet |
Marcela M. Satow Edite H. Yamashiro-Kanashiro Mussya C. Rocha Luiza K. Oyafuso Rita C. Soler Paulo C. Cotrim Jose Angelo L. Lindoso |
author_sort |
Marcela M. Satow |
title |
APPLICABILITY OF kDNA-PCR FOR ROUTINE DIAGNOSIS OF AMERICAN TEGUMENTARY LEISHMANIASIS IN A TERTIARY REFERENCE HOSPITAL |
title_short |
APPLICABILITY OF kDNA-PCR FOR ROUTINE DIAGNOSIS OF AMERICAN TEGUMENTARY LEISHMANIASIS IN A TERTIARY REFERENCE HOSPITAL |
title_full |
APPLICABILITY OF kDNA-PCR FOR ROUTINE DIAGNOSIS OF AMERICAN TEGUMENTARY LEISHMANIASIS IN A TERTIARY REFERENCE HOSPITAL |
title_fullStr |
APPLICABILITY OF kDNA-PCR FOR ROUTINE DIAGNOSIS OF AMERICAN TEGUMENTARY LEISHMANIASIS IN A TERTIARY REFERENCE HOSPITAL |
title_full_unstemmed |
APPLICABILITY OF kDNA-PCR FOR ROUTINE DIAGNOSIS OF AMERICAN TEGUMENTARY LEISHMANIASIS IN A TERTIARY REFERENCE HOSPITAL |
title_sort |
applicability of kdna-pcr for routine diagnosis of american tegumentary leishmaniasis in a tertiary reference hospital |
publisher |
Universidade de São Paulo (USP) |
publishDate |
2013 |
url |
https://doi.org/10.1590/S0036-46652013000600004 https://doaj.org/article/3e4d6edd2f9c40f39ee4fac52ef7bf6d |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Revista do Instituto de Medicina Tropical de São Paulo, Vol 55, Iss 6, Pp 393-399 (2013) |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0036-46652013000600393&lng=en&tlng=en https://doaj.org/toc/1678-9946 1678-9946 doi:10.1590/S0036-46652013000600004 https://doaj.org/article/3e4d6edd2f9c40f39ee4fac52ef7bf6d |
op_doi |
https://doi.org/10.1590/S0036-46652013000600004 |
container_title |
Revista do Instituto de Medicina Tropical de São Paulo |
container_volume |
55 |
container_issue |
6 |
container_start_page |
393 |
op_container_end_page |
399 |
_version_ |
1809895324626452480 |