Standardization of an enzyme linked immunosorbent assay (ELISA) for detecting circulating toxic venom antigens in patients stung by the scorpion Tityus serrulatus Padronização de um teste imunoenzimático (ELISA) para detectar antígenos tóxicos circulantes do veneno em pacientes picados pelo escorpião Tityus serrulatus
The sensitivity and specificity of an enzyme-linked immunosorbent assay (ELISA) for the detection of circulating antigens from toxic components of Tityus serrulatus scorpion venom was determined in patients stung by T. serrulatus before antivenom administration. Thirty-seven patients were classified...
Published in: | Revista do Instituto de Medicina Tropical de São Paulo |
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Universidade de São Paulo (USP)
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ftdoajarticles:oai:doaj.org/article:3dfccc2bfab24b468a43dba4f79bef17 2024-09-09T19:28:27+00:00 Standardization of an enzyme linked immunosorbent assay (ELISA) for detecting circulating toxic venom antigens in patients stung by the scorpion Tityus serrulatus Padronização de um teste imunoenzimático (ELISA) para detectar antígenos tóxicos circulantes do veneno em pacientes picados pelo escorpião Tityus serrulatus Nilton Alves de Rezende Mariana Borges Dias Délio Campolina Carlos Chavéz-Olortegui Carlos Faria Santos Amaral 1995-02-01T00:00:00Z https://doi.org/10.1590/S0036-46651995000100011 https://doaj.org/article/3dfccc2bfab24b468a43dba4f79bef17 EN eng Universidade de São Paulo (USP) http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0036-46651995000100011 https://doaj.org/toc/0036-4665 https://doaj.org/toc/1678-9946 doi:10.1590/S0036-46651995000100011 0036-4665 1678-9946 https://doaj.org/article/3dfccc2bfab24b468a43dba4f79bef17 Revista do Instituto de Medicina Tropical de São Paulo, Vol 37, Iss 1, Pp 71-74 (1995) Immunodiagnosis Scorpion envenoming Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 1995 ftdoajarticles https://doi.org/10.1590/S0036-46651995000100011 2024-08-05T17:49:30Z The sensitivity and specificity of an enzyme-linked immunosorbent assay (ELISA) for the detection of circulating antigens from toxic components of Tityus serrulatus scorpion venom was determined in patients stung by T. serrulatus before antivenom administration. Thirty-seven patients were classified as mild cases and 19 as moderate or severe cases. The control absorbance in the venom assay was provided by serum samples from 100 individuals of same socioeconomic group and geographical area who had never been stung by scorpions or treated with horse antisera. The negative cutoff value (mean + 2 SD) corresponded to a venom concentration of 4.8 ng/ml. Three out of the 100 normal sera were positive, resulting in a specificity of 97%. The sensitivity of the ELISA when all cases of scorpion sting were included was 39.3%. When mild cases were excluded, the sensitivity increased to 94.7%. This study showed that this ELISA can be used for the detection of circulating venom toxic antigens in patients with systemic manifestations following. T. serrulatus sting but cannot be used for clinical studies in mild cases of envenoming since the test does not discriminate mild cases from control patients. Neste trabalho foram determinadas a sensibilidade e a especificidade da técnica imunoenzimática (ELISA) desenvolvida por CHAVÉZ-OLORTEGUI et al. para detectar antígenos circulantes de veneno em pacientes picados po Tityus serrulatus. A média mais dois desvios padrão da observância do soro de 100 pacientes controles foi utilizada como limite entre teste positivo e teste negativo ("cutoff"). A especificidade do ELISA foi igual a 97,0%. A sensibilidade do método, quando incluidos pacientes classificados como casos leves, moderados e graves de escorpionismo, foi de 39,3% e aumentou para 94,7% quando considerados apenas os casos moderados e graves. Estes resultados mostram que o ELISA pode ser utilizado para detecção de antígenos tóxicos circulantes em pacientes com manifestações sistêmicas de envenenamento escorpiônico mas não deve ser ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Revista do Instituto de Medicina Tropical de São Paulo 37 1 71 74 |
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Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Immunodiagnosis Scorpion envenoming Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
spellingShingle |
Immunodiagnosis Scorpion envenoming Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 Nilton Alves de Rezende Mariana Borges Dias Délio Campolina Carlos Chavéz-Olortegui Carlos Faria Santos Amaral Standardization of an enzyme linked immunosorbent assay (ELISA) for detecting circulating toxic venom antigens in patients stung by the scorpion Tityus serrulatus Padronização de um teste imunoenzimático (ELISA) para detectar antígenos tóxicos circulantes do veneno em pacientes picados pelo escorpião Tityus serrulatus |
topic_facet |
Immunodiagnosis Scorpion envenoming Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
The sensitivity and specificity of an enzyme-linked immunosorbent assay (ELISA) for the detection of circulating antigens from toxic components of Tityus serrulatus scorpion venom was determined in patients stung by T. serrulatus before antivenom administration. Thirty-seven patients were classified as mild cases and 19 as moderate or severe cases. The control absorbance in the venom assay was provided by serum samples from 100 individuals of same socioeconomic group and geographical area who had never been stung by scorpions or treated with horse antisera. The negative cutoff value (mean + 2 SD) corresponded to a venom concentration of 4.8 ng/ml. Three out of the 100 normal sera were positive, resulting in a specificity of 97%. The sensitivity of the ELISA when all cases of scorpion sting were included was 39.3%. When mild cases were excluded, the sensitivity increased to 94.7%. This study showed that this ELISA can be used for the detection of circulating venom toxic antigens in patients with systemic manifestations following. T. serrulatus sting but cannot be used for clinical studies in mild cases of envenoming since the test does not discriminate mild cases from control patients. Neste trabalho foram determinadas a sensibilidade e a especificidade da técnica imunoenzimática (ELISA) desenvolvida por CHAVÉZ-OLORTEGUI et al. para detectar antígenos circulantes de veneno em pacientes picados po Tityus serrulatus. A média mais dois desvios padrão da observância do soro de 100 pacientes controles foi utilizada como limite entre teste positivo e teste negativo ("cutoff"). A especificidade do ELISA foi igual a 97,0%. A sensibilidade do método, quando incluidos pacientes classificados como casos leves, moderados e graves de escorpionismo, foi de 39,3% e aumentou para 94,7% quando considerados apenas os casos moderados e graves. Estes resultados mostram que o ELISA pode ser utilizado para detecção de antígenos tóxicos circulantes em pacientes com manifestações sistêmicas de envenenamento escorpiônico mas não deve ser ... |
format |
Article in Journal/Newspaper |
author |
Nilton Alves de Rezende Mariana Borges Dias Délio Campolina Carlos Chavéz-Olortegui Carlos Faria Santos Amaral |
author_facet |
Nilton Alves de Rezende Mariana Borges Dias Délio Campolina Carlos Chavéz-Olortegui Carlos Faria Santos Amaral |
author_sort |
Nilton Alves de Rezende |
title |
Standardization of an enzyme linked immunosorbent assay (ELISA) for detecting circulating toxic venom antigens in patients stung by the scorpion Tityus serrulatus Padronização de um teste imunoenzimático (ELISA) para detectar antígenos tóxicos circulantes do veneno em pacientes picados pelo escorpião Tityus serrulatus |
title_short |
Standardization of an enzyme linked immunosorbent assay (ELISA) for detecting circulating toxic venom antigens in patients stung by the scorpion Tityus serrulatus Padronização de um teste imunoenzimático (ELISA) para detectar antígenos tóxicos circulantes do veneno em pacientes picados pelo escorpião Tityus serrulatus |
title_full |
Standardization of an enzyme linked immunosorbent assay (ELISA) for detecting circulating toxic venom antigens in patients stung by the scorpion Tityus serrulatus Padronização de um teste imunoenzimático (ELISA) para detectar antígenos tóxicos circulantes do veneno em pacientes picados pelo escorpião Tityus serrulatus |
title_fullStr |
Standardization of an enzyme linked immunosorbent assay (ELISA) for detecting circulating toxic venom antigens in patients stung by the scorpion Tityus serrulatus Padronização de um teste imunoenzimático (ELISA) para detectar antígenos tóxicos circulantes do veneno em pacientes picados pelo escorpião Tityus serrulatus |
title_full_unstemmed |
Standardization of an enzyme linked immunosorbent assay (ELISA) for detecting circulating toxic venom antigens in patients stung by the scorpion Tityus serrulatus Padronização de um teste imunoenzimático (ELISA) para detectar antígenos tóxicos circulantes do veneno em pacientes picados pelo escorpião Tityus serrulatus |
title_sort |
standardization of an enzyme linked immunosorbent assay (elisa) for detecting circulating toxic venom antigens in patients stung by the scorpion tityus serrulatus padronização de um teste imunoenzimático (elisa) para detectar antígenos tóxicos circulantes do veneno em pacientes picados pelo escorpião tityus serrulatus |
publisher |
Universidade de São Paulo (USP) |
publishDate |
1995 |
url |
https://doi.org/10.1590/S0036-46651995000100011 https://doaj.org/article/3dfccc2bfab24b468a43dba4f79bef17 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Revista do Instituto de Medicina Tropical de São Paulo, Vol 37, Iss 1, Pp 71-74 (1995) |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0036-46651995000100011 https://doaj.org/toc/0036-4665 https://doaj.org/toc/1678-9946 doi:10.1590/S0036-46651995000100011 0036-4665 1678-9946 https://doaj.org/article/3dfccc2bfab24b468a43dba4f79bef17 |
op_doi |
https://doi.org/10.1590/S0036-46651995000100011 |
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Revista do Instituto de Medicina Tropical de São Paulo |
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37 |
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