Establishment of an isolation method of Nostoc commune cells free from extracellular polysaccharides (EPS) using Percoll centrifugation
The terrestrial cyanobacterium Nostoc commune Vaucher ex Bornet et Flahault occurs worldwide, including in Japan and Antarctica. N. commune has a large amount of extracellular polysaccharides (EPS) that hold moisture and protect the cells and at the same time accumulate light-blocking substances whi...
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ftdoajarticles:oai:doaj.org/article:3daea65796cb4f28be781dab5787f454 2023-05-15T13:48:51+02:00 Establishment of an isolation method of Nostoc commune cells free from extracellular polysaccharides (EPS) using Percoll centrifugation Makiko Kosugi Yasuhiro Kashino Sakae Kudoh Satoshi Imura 2012-11-01T00:00:00Z https://doi.org/10.15094/00009663 https://doaj.org/article/3daea65796cb4f28be781dab5787f454 EN JA eng jpn National Institute of Polar Research http://doi.org/10.15094/00009663 https://doaj.org/toc/0085-7289 https://doaj.org/toc/2432-079X doi:10.15094/00009663 0085-7289 2432-079X https://doaj.org/article/3daea65796cb4f28be781dab5787f454 Antarctic Record, Vol 56, Iss 3, Pp 285-293 (2012) Geography (General) G1-922 article 2012 ftdoajarticles https://doi.org/10.15094/00009663 2022-12-31T12:39:39Z The terrestrial cyanobacterium Nostoc commune Vaucher ex Bornet et Flahault occurs worldwide, including in Japan and Antarctica. N. commune has a large amount of extracellular polysaccharides (EPS) that hold moisture and protect the cells and at the same time accumulate light-blocking substances which is believed to play an important part in adaptation to a severe environment. To evaluate the photoadaptation processes in N. commune and clarify the role(s) of EPS under ambient environmental condition at Antarctica, separation of cells from EPS is necessary. High yield is a prerequisite for the use of only small amount of natural N. commune from Antarctica. For this purpose, we developed a separation method by improving the Percoll density gradient centrifugation method using an EPS-coated field-grown Nostoc population. We established the most suitable condition to separate naked cells from EPS at high yield retaining high photosynthetic activity. The method is composed of centrifugation of cell homogenated N. commune in 10% (v/v) Percoll to separate cells efficiently from EPS followed by fractionating centrifugation to remove impurities using the gradient of Percoll (80% and 50%, v/v). Article in Journal/Newspaper Antarc* Antarctica Directory of Open Access Journals: DOAJ Articles |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
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English Japanese |
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Geography (General) G1-922 |
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Geography (General) G1-922 Makiko Kosugi Yasuhiro Kashino Sakae Kudoh Satoshi Imura Establishment of an isolation method of Nostoc commune cells free from extracellular polysaccharides (EPS) using Percoll centrifugation |
topic_facet |
Geography (General) G1-922 |
description |
The terrestrial cyanobacterium Nostoc commune Vaucher ex Bornet et Flahault occurs worldwide, including in Japan and Antarctica. N. commune has a large amount of extracellular polysaccharides (EPS) that hold moisture and protect the cells and at the same time accumulate light-blocking substances which is believed to play an important part in adaptation to a severe environment. To evaluate the photoadaptation processes in N. commune and clarify the role(s) of EPS under ambient environmental condition at Antarctica, separation of cells from EPS is necessary. High yield is a prerequisite for the use of only small amount of natural N. commune from Antarctica. For this purpose, we developed a separation method by improving the Percoll density gradient centrifugation method using an EPS-coated field-grown Nostoc population. We established the most suitable condition to separate naked cells from EPS at high yield retaining high photosynthetic activity. The method is composed of centrifugation of cell homogenated N. commune in 10% (v/v) Percoll to separate cells efficiently from EPS followed by fractionating centrifugation to remove impurities using the gradient of Percoll (80% and 50%, v/v). |
format |
Article in Journal/Newspaper |
author |
Makiko Kosugi Yasuhiro Kashino Sakae Kudoh Satoshi Imura |
author_facet |
Makiko Kosugi Yasuhiro Kashino Sakae Kudoh Satoshi Imura |
author_sort |
Makiko Kosugi |
title |
Establishment of an isolation method of Nostoc commune cells free from extracellular polysaccharides (EPS) using Percoll centrifugation |
title_short |
Establishment of an isolation method of Nostoc commune cells free from extracellular polysaccharides (EPS) using Percoll centrifugation |
title_full |
Establishment of an isolation method of Nostoc commune cells free from extracellular polysaccharides (EPS) using Percoll centrifugation |
title_fullStr |
Establishment of an isolation method of Nostoc commune cells free from extracellular polysaccharides (EPS) using Percoll centrifugation |
title_full_unstemmed |
Establishment of an isolation method of Nostoc commune cells free from extracellular polysaccharides (EPS) using Percoll centrifugation |
title_sort |
establishment of an isolation method of nostoc commune cells free from extracellular polysaccharides (eps) using percoll centrifugation |
publisher |
National Institute of Polar Research |
publishDate |
2012 |
url |
https://doi.org/10.15094/00009663 https://doaj.org/article/3daea65796cb4f28be781dab5787f454 |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_source |
Antarctic Record, Vol 56, Iss 3, Pp 285-293 (2012) |
op_relation |
http://doi.org/10.15094/00009663 https://doaj.org/toc/0085-7289 https://doaj.org/toc/2432-079X doi:10.15094/00009663 0085-7289 2432-079X https://doaj.org/article/3daea65796cb4f28be781dab5787f454 |
op_doi |
https://doi.org/10.15094/00009663 |
_version_ |
1766249854032412672 |