Identification of surrogates of protection against yersiniosis in immersion vaccinated Atlantic salmon.
Simple cost-effective bacterins are the earliest and most successfully used commercial vaccines in fish. In particular, those prepared from Yersinia ruckeri have proven effective at controlling Enteric Red Mouth Disease (ERM) and yersiniosis in rainbow trout and Atlantic salmon, respectively. Howeve...
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ftdoajarticles:oai:doaj.org/article:3da45678604741f6bb13c007b53e29c9 2023-05-15T15:29:58+02:00 Identification of surrogates of protection against yersiniosis in immersion vaccinated Atlantic salmon. Andrew R Bridle Ben F Koop Barbara F Nowak 2012-01-01T00:00:00Z https://doi.org/10.1371/journal.pone.0040841 https://doaj.org/article/3da45678604741f6bb13c007b53e29c9 EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC3395641?pdf=render https://doaj.org/toc/1932-6203 1932-6203 doi:10.1371/journal.pone.0040841 https://doaj.org/article/3da45678604741f6bb13c007b53e29c9 PLoS ONE, Vol 7, Iss 7, p e40841 (2012) Medicine R Science Q article 2012 ftdoajarticles https://doi.org/10.1371/journal.pone.0040841 2022-12-31T03:52:35Z Simple cost-effective bacterins are the earliest and most successfully used commercial vaccines in fish. In particular, those prepared from Yersinia ruckeri have proven effective at controlling Enteric Red Mouth Disease (ERM) and yersiniosis in rainbow trout and Atlantic salmon, respectively. However, the emergence of outbreaks of ERM caused by atypical biotypes of Y. ruckeri and reports of vaccine failure resulting in mass mortality of hatchery Atlantic salmon has reinvigorated interest in vaccines against fish bacterial diseases. Therefore the objective of this study was to identify surrogates of protection against yersiniosis using cDNA microarray to characterise the response of host genes in the gills of unvaccinated and vaccinated Atlantic salmon challenged with Y. ruckeri. Differentially expressed genes were identified using two-way ANOVA and restricted to those with >2.5-fold change at P<0.05. Using cDNA microarray we identified the expression of 6 genes in response to infection and 4 genes associated with the protective host response to yersiniosis. Analysis by real-time PCR confirmed that three immunologically relevant genes, namely a cathelicidin (47-fold) and a C-type lectin (19-fold) increased in response to yersiniosis. Including collagenase (17-fold increase), an important tissue remodelling and repair enzyme, these genes represent 3 of 6 non-protective and/or pathological responses to yersiniosis. Genes associated with the protective host response included an immunoglobulin gene and a selenoprotein that showed significant fold changes (15-fold increases each), highlighting the importance of antibody-mediated protection against yersiniosis. These findings provide much needed knowledge of the host-pathogen interaction in response to bacterial infection and immunisation in fish. Significantly, we identified a transcriptional biosignature consisting of predominantly immune-relevant genes (14 up and 3 down-regulated) in the gills of Atlantic salmon after immersion vaccination and before bacterial ... Article in Journal/Newspaper Atlantic salmon Directory of Open Access Journals: DOAJ Articles PLoS ONE 7 7 e40841 |
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Medicine R Science Q Andrew R Bridle Ben F Koop Barbara F Nowak Identification of surrogates of protection against yersiniosis in immersion vaccinated Atlantic salmon. |
topic_facet |
Medicine R Science Q |
description |
Simple cost-effective bacterins are the earliest and most successfully used commercial vaccines in fish. In particular, those prepared from Yersinia ruckeri have proven effective at controlling Enteric Red Mouth Disease (ERM) and yersiniosis in rainbow trout and Atlantic salmon, respectively. However, the emergence of outbreaks of ERM caused by atypical biotypes of Y. ruckeri and reports of vaccine failure resulting in mass mortality of hatchery Atlantic salmon has reinvigorated interest in vaccines against fish bacterial diseases. Therefore the objective of this study was to identify surrogates of protection against yersiniosis using cDNA microarray to characterise the response of host genes in the gills of unvaccinated and vaccinated Atlantic salmon challenged with Y. ruckeri. Differentially expressed genes were identified using two-way ANOVA and restricted to those with >2.5-fold change at P<0.05. Using cDNA microarray we identified the expression of 6 genes in response to infection and 4 genes associated with the protective host response to yersiniosis. Analysis by real-time PCR confirmed that three immunologically relevant genes, namely a cathelicidin (47-fold) and a C-type lectin (19-fold) increased in response to yersiniosis. Including collagenase (17-fold increase), an important tissue remodelling and repair enzyme, these genes represent 3 of 6 non-protective and/or pathological responses to yersiniosis. Genes associated with the protective host response included an immunoglobulin gene and a selenoprotein that showed significant fold changes (15-fold increases each), highlighting the importance of antibody-mediated protection against yersiniosis. These findings provide much needed knowledge of the host-pathogen interaction in response to bacterial infection and immunisation in fish. Significantly, we identified a transcriptional biosignature consisting of predominantly immune-relevant genes (14 up and 3 down-regulated) in the gills of Atlantic salmon after immersion vaccination and before bacterial ... |
format |
Article in Journal/Newspaper |
author |
Andrew R Bridle Ben F Koop Barbara F Nowak |
author_facet |
Andrew R Bridle Ben F Koop Barbara F Nowak |
author_sort |
Andrew R Bridle |
title |
Identification of surrogates of protection against yersiniosis in immersion vaccinated Atlantic salmon. |
title_short |
Identification of surrogates of protection against yersiniosis in immersion vaccinated Atlantic salmon. |
title_full |
Identification of surrogates of protection against yersiniosis in immersion vaccinated Atlantic salmon. |
title_fullStr |
Identification of surrogates of protection against yersiniosis in immersion vaccinated Atlantic salmon. |
title_full_unstemmed |
Identification of surrogates of protection against yersiniosis in immersion vaccinated Atlantic salmon. |
title_sort |
identification of surrogates of protection against yersiniosis in immersion vaccinated atlantic salmon. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2012 |
url |
https://doi.org/10.1371/journal.pone.0040841 https://doaj.org/article/3da45678604741f6bb13c007b53e29c9 |
genre |
Atlantic salmon |
genre_facet |
Atlantic salmon |
op_source |
PLoS ONE, Vol 7, Iss 7, p e40841 (2012) |
op_relation |
http://europepmc.org/articles/PMC3395641?pdf=render https://doaj.org/toc/1932-6203 1932-6203 doi:10.1371/journal.pone.0040841 https://doaj.org/article/3da45678604741f6bb13c007b53e29c9 |
op_doi |
https://doi.org/10.1371/journal.pone.0040841 |
container_title |
PLoS ONE |
container_volume |
7 |
container_issue |
7 |
container_start_page |
e40841 |
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