Identification of toxocara canis antigens by Western blot in experimentally infected rabbits
Toxocariasis is a frequent helminthiasis that can cause visceral and ocular damage in humans specially in children. The identification of specific antigens of Toxocara canis is important in order to develop better diagnostic techniques. Ten rabbits were infected orally with a dose of 5000 Toxocara c...
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Universidade de São Paulo (USP)
2002
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ftdoajarticles:oai:doaj.org/article:3d8315b0852f4d30a7f9ac8bb8f9265b 2024-09-09T19:26:39+00:00 Identification of toxocara canis antigens by Western blot in experimentally infected rabbits MORALES Olga Lucía LÓPEZ Myriam Consuelo NICHOLLS Rubén Santiago AGUDELO Carlos 2002-01-01T00:00:00Z https://doaj.org/article/3d8315b0852f4d30a7f9ac8bb8f9265b EN eng Universidade de São Paulo (USP) http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0036-46652002000400006 https://doaj.org/toc/0036-4665 https://doaj.org/toc/1678-9946 0036-4665 1678-9946 https://doaj.org/article/3d8315b0852f4d30a7f9ac8bb8f9265b Revista do Instituto de Medicina Tropical de São Paulo, Vol 44, Iss 4, Pp 213-216 (2002) Toxocara canis Immunoblot ELISA Antibodies Diagnosis Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 2002 ftdoajarticles 2024-08-05T17:49:30Z Toxocariasis is a frequent helminthiasis that can cause visceral and ocular damage in humans specially in children. The identification of specific antigens of Toxocara canis is important in order to develop better diagnostic techniques. Ten rabbits were infected orally with a dose of 5000 Toxocara canis embryonated eggs. Rabbits were bled periodically and an ELISA assay was performed to determine levels of specific Toxocara IgG antibodies. ELISA detected antibodies at day 15 after infection. Western blot (WB) assay was performed using excretory/secretory antigens (E/S) of T. canis second stage larvae. Different antigen concentrations were evaluated: 150, 200, 250 and 300 µg/mL. The concentration of 250 µg/mL was retained for analysis. Rabbit sera were diluted 1:100. Secondary antibody was used at a dilution of 1:1000. Results of WB indicated that in the first month after infection specific antibodies against the 200 KDa, 116 KDa, 92 KDa and 35 KDa antigens were detected; antibodies against the 92 KDa, 80 KDa, 66 KDa, 45 KDa, 31 KDa and 28 KDa antigens appeared later. All positive sera in the ELISA test were also positive in WB. Two antigen bands, 92 KDa and 35 KDa, were identified since the beginning and throughout the course of infection. These antigens merit further evaluation as candidates for use in diagnosis. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic |
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Toxocara canis Immunoblot ELISA Antibodies Diagnosis Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
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Toxocara canis Immunoblot ELISA Antibodies Diagnosis Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 MORALES Olga Lucía LÓPEZ Myriam Consuelo NICHOLLS Rubén Santiago AGUDELO Carlos Identification of toxocara canis antigens by Western blot in experimentally infected rabbits |
topic_facet |
Toxocara canis Immunoblot ELISA Antibodies Diagnosis Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
Toxocariasis is a frequent helminthiasis that can cause visceral and ocular damage in humans specially in children. The identification of specific antigens of Toxocara canis is important in order to develop better diagnostic techniques. Ten rabbits were infected orally with a dose of 5000 Toxocara canis embryonated eggs. Rabbits were bled periodically and an ELISA assay was performed to determine levels of specific Toxocara IgG antibodies. ELISA detected antibodies at day 15 after infection. Western blot (WB) assay was performed using excretory/secretory antigens (E/S) of T. canis second stage larvae. Different antigen concentrations were evaluated: 150, 200, 250 and 300 µg/mL. The concentration of 250 µg/mL was retained for analysis. Rabbit sera were diluted 1:100. Secondary antibody was used at a dilution of 1:1000. Results of WB indicated that in the first month after infection specific antibodies against the 200 KDa, 116 KDa, 92 KDa and 35 KDa antigens were detected; antibodies against the 92 KDa, 80 KDa, 66 KDa, 45 KDa, 31 KDa and 28 KDa antigens appeared later. All positive sera in the ELISA test were also positive in WB. Two antigen bands, 92 KDa and 35 KDa, were identified since the beginning and throughout the course of infection. These antigens merit further evaluation as candidates for use in diagnosis. |
format |
Article in Journal/Newspaper |
author |
MORALES Olga Lucía LÓPEZ Myriam Consuelo NICHOLLS Rubén Santiago AGUDELO Carlos |
author_facet |
MORALES Olga Lucía LÓPEZ Myriam Consuelo NICHOLLS Rubén Santiago AGUDELO Carlos |
author_sort |
MORALES Olga Lucía |
title |
Identification of toxocara canis antigens by Western blot in experimentally infected rabbits |
title_short |
Identification of toxocara canis antigens by Western blot in experimentally infected rabbits |
title_full |
Identification of toxocara canis antigens by Western blot in experimentally infected rabbits |
title_fullStr |
Identification of toxocara canis antigens by Western blot in experimentally infected rabbits |
title_full_unstemmed |
Identification of toxocara canis antigens by Western blot in experimentally infected rabbits |
title_sort |
identification of toxocara canis antigens by western blot in experimentally infected rabbits |
publisher |
Universidade de São Paulo (USP) |
publishDate |
2002 |
url |
https://doaj.org/article/3d8315b0852f4d30a7f9ac8bb8f9265b |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Revista do Instituto de Medicina Tropical de São Paulo, Vol 44, Iss 4, Pp 213-216 (2002) |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0036-46652002000400006 https://doaj.org/toc/0036-4665 https://doaj.org/toc/1678-9946 0036-4665 1678-9946 https://doaj.org/article/3d8315b0852f4d30a7f9ac8bb8f9265b |
_version_ |
1809896219909029888 |