Antibacterial activity of marine bacteria isolated from sponge Xestospongia testudinaria from Sorong, Papua

• Published in: Asian Pacific Journal of Tropical Biomedicine
• Main Authors: Yatnita Parama Cita, Achmad Suhermanto, Ocky Karna Radjasa, Pratiwi Sudharmono
• Format: Article in Journal/Newspaper
• Language: English
• Published: Wolters Kluwer Medknow Publications 2017
• Subjects: Antibacterial activity; Marine bacteria; Xestospongia testudinaria; Sorong; Arctic medicine. Tropical medicine; RC955-962; Biology (General); QH301-705.5; Arctic
• Online Access: https://doi.org/10.1016/j.apjtb.2017.01.024; https://doaj.org/article/3d64ea9e85e34783abaa79a87c778215

Objective: To explore secondary metabolite of bacteria-associated Xestospongia testudinaria from Tanjung Kasuari, Sorong, Papua. Methods: The antimicrobial activities of extracts against two Gram-positive bacteria (Staphylococcus aureus) and three Gram-negative bacteria (Pseudomonas aeruginosa, Eschericia coli and Salmonella typhi) were determined by disk diffusion dilution method. Results: The test showed that of 15 isolates of symbiont bacteria, 6 isolates were successfully isolated and coded, namely, Xp 4.1, Xp 4.2, Xp 4.3, Xp 4.4, Xp 4.5 and Xp 4.6. Of the six bacterial isolates, isolated Xp 4.2 was found to have more powerful antibacterial activity than any other isolates of symbiont bacteria. Antibacterial activity assay for the n-hexane soluble fractions, ethyl-acetate soluble fractions, and n-buthanol soluble fractions revealed more powerful anti-bacterial activity than any other soluble fractions. Phytochemical screening showed alkaloid and steroid/triterpenoid, while identification for isolate of Xp 4.2 bacterial showed bacteria. Conclusions: Metabolites of bacterial associated with marine sponge Xestospongia testudinaria promise to be developed into antibacterial agents.