Characterization of Plasmodium falciparum genes associated with drug resistance in Hodh Elgharbi, a malaria hotspot near Malian–Mauritanian border
Abstract Background A malaria hotspot in the southeastern region of Mauritania, near the Malian border, may hamper malaria control strategies. The objectives were to estimate the prevalence of genetic polymorphisms associated with drug resistance in Plasmodium falciparum isolates and establish basel...
Published in: | Malaria Journal |
---|---|
Main Authors: | , , , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
BMC
2017
|
Subjects: | |
Online Access: | https://doi.org/10.1186/s12936-017-1791-2 https://doaj.org/article/3d5b54642454430690d06052cf369cfb |
id |
ftdoajarticles:oai:doaj.org/article:3d5b54642454430690d06052cf369cfb |
---|---|
record_format |
openpolar |
spelling |
ftdoajarticles:oai:doaj.org/article:3d5b54642454430690d06052cf369cfb 2023-05-15T15:15:42+02:00 Characterization of Plasmodium falciparum genes associated with drug resistance in Hodh Elgharbi, a malaria hotspot near Malian–Mauritanian border Mohamed Salem Ould Ahmedou Salem Khadijetou Mint Lekweiry Houssem Bouchiba Aurelie Pascual Bruno Pradines Ali Ould Mohamed Salem Boukhary Sébastien Briolant Leonardo K. Basco Hervé Bogreau 2017-04-01T00:00:00Z https://doi.org/10.1186/s12936-017-1791-2 https://doaj.org/article/3d5b54642454430690d06052cf369cfb EN eng BMC http://link.springer.com/article/10.1186/s12936-017-1791-2 https://doaj.org/toc/1475-2875 doi:10.1186/s12936-017-1791-2 1475-2875 https://doaj.org/article/3d5b54642454430690d06052cf369cfb Malaria Journal, Vol 16, Iss 1, Pp 1-9 (2017) Plasmodium falciparum Drug resistance Chloroquine Amodiaquine Antifolate drugs Lumefantrine Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 2017 ftdoajarticles https://doi.org/10.1186/s12936-017-1791-2 2022-12-31T00:48:53Z Abstract Background A malaria hotspot in the southeastern region of Mauritania, near the Malian border, may hamper malaria control strategies. The objectives were to estimate the prevalence of genetic polymorphisms associated with drug resistance in Plasmodium falciparum isolates and establish baseline data. Methods The study was conducted in two malaria-endemic areas in Hodh Elgharbi, situated in the Malian–Mauritanian border area. Blood samples were collected from symptomatic patients. Single nucleotide polymorphisms in Pfcrt, Pfmdr1, Pfdhfr, and Pfdhps were genotyped using PCR-restriction fragment length polymorphism, DNA sequencing and primer extension. The Pfmdr1 gene copy number was determined by real-time PCR. Results Of 280 P. falciparum-infected patients, 193 (68.9%) carried the Pfcrt 76T mutant allele. The Pfmdr1 86Y and 184F mutations were found in 61 (23.1%) of 264 isolates and 167 (67.6%) of 247 samples that were successfully genotyped, respectively. Pfmdr1 mutant alleles 1034C, 1042D and 1246Y were rarely observed. Of 102 P. falciparum isolates analysed, ten (9.8%) had more than one copy of Pfmdr1 gene. The prevalence of isolates harbouring at least triple mutant Pfdhfr 51I, 59R, 108 N/T was 42% (112/268), of which 42 (37.5%) had an additional Pfdhps 437G mutation. The Pfdhps 540E mutation was observed in four isolates (1.5%), including three associated with Pfdhfr triple mutant. Only two quintuple mutants (Pfdhfr-51I-59R-108N Pfdhps-437G-540E) were observed. Conclusions The observed mutations in Pfdhfr, Pfdhps, Pfmdr1, and Pfcrt may jeopardize the future of seasonal malaria chemoprevention based on amodiaquine-sulfadoxine-pyrimethamine, intermittent preventive treatment for pregnant women using sulfadoxine-pyrimethamine, and treatment with artesunate-amodiaquine. Complementary studies should be carried out to document the distribution, origin and circulation of P. falciparum populations in this region and more widely in the country to assess the risk of the spread of resistance. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Malaria Journal 16 1 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Plasmodium falciparum Drug resistance Chloroquine Amodiaquine Antifolate drugs Lumefantrine Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
spellingShingle |
Plasmodium falciparum Drug resistance Chloroquine Amodiaquine Antifolate drugs Lumefantrine Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 Mohamed Salem Ould Ahmedou Salem Khadijetou Mint Lekweiry Houssem Bouchiba Aurelie Pascual Bruno Pradines Ali Ould Mohamed Salem Boukhary Sébastien Briolant Leonardo K. Basco Hervé Bogreau Characterization of Plasmodium falciparum genes associated with drug resistance in Hodh Elgharbi, a malaria hotspot near Malian–Mauritanian border |
topic_facet |
Plasmodium falciparum Drug resistance Chloroquine Amodiaquine Antifolate drugs Lumefantrine Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
Abstract Background A malaria hotspot in the southeastern region of Mauritania, near the Malian border, may hamper malaria control strategies. The objectives were to estimate the prevalence of genetic polymorphisms associated with drug resistance in Plasmodium falciparum isolates and establish baseline data. Methods The study was conducted in two malaria-endemic areas in Hodh Elgharbi, situated in the Malian–Mauritanian border area. Blood samples were collected from symptomatic patients. Single nucleotide polymorphisms in Pfcrt, Pfmdr1, Pfdhfr, and Pfdhps were genotyped using PCR-restriction fragment length polymorphism, DNA sequencing and primer extension. The Pfmdr1 gene copy number was determined by real-time PCR. Results Of 280 P. falciparum-infected patients, 193 (68.9%) carried the Pfcrt 76T mutant allele. The Pfmdr1 86Y and 184F mutations were found in 61 (23.1%) of 264 isolates and 167 (67.6%) of 247 samples that were successfully genotyped, respectively. Pfmdr1 mutant alleles 1034C, 1042D and 1246Y were rarely observed. Of 102 P. falciparum isolates analysed, ten (9.8%) had more than one copy of Pfmdr1 gene. The prevalence of isolates harbouring at least triple mutant Pfdhfr 51I, 59R, 108 N/T was 42% (112/268), of which 42 (37.5%) had an additional Pfdhps 437G mutation. The Pfdhps 540E mutation was observed in four isolates (1.5%), including three associated with Pfdhfr triple mutant. Only two quintuple mutants (Pfdhfr-51I-59R-108N Pfdhps-437G-540E) were observed. Conclusions The observed mutations in Pfdhfr, Pfdhps, Pfmdr1, and Pfcrt may jeopardize the future of seasonal malaria chemoprevention based on amodiaquine-sulfadoxine-pyrimethamine, intermittent preventive treatment for pregnant women using sulfadoxine-pyrimethamine, and treatment with artesunate-amodiaquine. Complementary studies should be carried out to document the distribution, origin and circulation of P. falciparum populations in this region and more widely in the country to assess the risk of the spread of resistance. |
format |
Article in Journal/Newspaper |
author |
Mohamed Salem Ould Ahmedou Salem Khadijetou Mint Lekweiry Houssem Bouchiba Aurelie Pascual Bruno Pradines Ali Ould Mohamed Salem Boukhary Sébastien Briolant Leonardo K. Basco Hervé Bogreau |
author_facet |
Mohamed Salem Ould Ahmedou Salem Khadijetou Mint Lekweiry Houssem Bouchiba Aurelie Pascual Bruno Pradines Ali Ould Mohamed Salem Boukhary Sébastien Briolant Leonardo K. Basco Hervé Bogreau |
author_sort |
Mohamed Salem Ould Ahmedou Salem |
title |
Characterization of Plasmodium falciparum genes associated with drug resistance in Hodh Elgharbi, a malaria hotspot near Malian–Mauritanian border |
title_short |
Characterization of Plasmodium falciparum genes associated with drug resistance in Hodh Elgharbi, a malaria hotspot near Malian–Mauritanian border |
title_full |
Characterization of Plasmodium falciparum genes associated with drug resistance in Hodh Elgharbi, a malaria hotspot near Malian–Mauritanian border |
title_fullStr |
Characterization of Plasmodium falciparum genes associated with drug resistance in Hodh Elgharbi, a malaria hotspot near Malian–Mauritanian border |
title_full_unstemmed |
Characterization of Plasmodium falciparum genes associated with drug resistance in Hodh Elgharbi, a malaria hotspot near Malian–Mauritanian border |
title_sort |
characterization of plasmodium falciparum genes associated with drug resistance in hodh elgharbi, a malaria hotspot near malian–mauritanian border |
publisher |
BMC |
publishDate |
2017 |
url |
https://doi.org/10.1186/s12936-017-1791-2 https://doaj.org/article/3d5b54642454430690d06052cf369cfb |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Malaria Journal, Vol 16, Iss 1, Pp 1-9 (2017) |
op_relation |
http://link.springer.com/article/10.1186/s12936-017-1791-2 https://doaj.org/toc/1475-2875 doi:10.1186/s12936-017-1791-2 1475-2875 https://doaj.org/article/3d5b54642454430690d06052cf369cfb |
op_doi |
https://doi.org/10.1186/s12936-017-1791-2 |
container_title |
Malaria Journal |
container_volume |
16 |
container_issue |
1 |
_version_ |
1766346045983293440 |