Development of 3D Printed Enzymatic Microreactors for Lipase-Catalyzed Reactions in Deep Eutectic Solvent-Based Media
In this study, 3D printing technology was exploited for the development of immobilized enzyme microreactors that could be used for biocatalytic processes in Deep Eutectic Solvent (DES)-based media. 3D-printed polylactic acid (PLA) microwell plates or tubular microfluidic reactors were modified with...
Published in: | Micromachines |
---|---|
Main Authors: | , , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
MDPI AG
2022
|
Subjects: | |
Online Access: | https://doi.org/10.3390/mi13111954 https://doaj.org/article/3d54868b3a154dd98bb6f4f797d52448 |
id |
ftdoajarticles:oai:doaj.org/article:3d54868b3a154dd98bb6f4f797d52448 |
---|---|
record_format |
openpolar |
spelling |
ftdoajarticles:oai:doaj.org/article:3d54868b3a154dd98bb6f4f797d52448 2023-05-15T13:55:48+02:00 Development of 3D Printed Enzymatic Microreactors for Lipase-Catalyzed Reactions in Deep Eutectic Solvent-Based Media Myrto G. Bellou Elena Gkantzou Anastasia Skonta Dimitrios Moschovas Konstantinos Spyrou Apostolos Avgeropoulos Dimitrios Gournis Haralambos Stamatis 2022-11-01T00:00:00Z https://doi.org/10.3390/mi13111954 https://doaj.org/article/3d54868b3a154dd98bb6f4f797d52448 EN eng MDPI AG https://www.mdpi.com/2072-666X/13/11/1954 https://doaj.org/toc/2072-666X doi:10.3390/mi13111954 2072-666X https://doaj.org/article/3d54868b3a154dd98bb6f4f797d52448 Micromachines, Vol 13, Iss 1954, p 1954 (2022) 3D printing immobilization enzyme microreactor deep eutectic solvents lipase Mechanical engineering and machinery TJ1-1570 article 2022 ftdoajarticles https://doi.org/10.3390/mi13111954 2022-12-30T21:15:38Z In this study, 3D printing technology was exploited for the development of immobilized enzyme microreactors that could be used for biocatalytic processes in Deep Eutectic Solvent (DES)-based media. 3D-printed polylactic acid (PLA) microwell plates or tubular microfluidic reactors were modified with polyethylenimine (PEI) and lipase from Candida antarctica (CALB) was covalently immobilized in the interior of each structure. DESs were found to have a negligible effect on the activity and stability of CALB, and the system proved highly stable and reusable in the presence of DESs for the hydrolysis of p-nitrophenyl butyrate (p-NPB). A kinetic study under flow conditions revealed an enhancement of substrate accessibility in the presence of Betaine: Glycerol (Bet:Gly) DES, while the system was not severely affected by diffusion limitations. Incubation of microreactors in 100% Bet:Gly preserved the enzyme activity by 53% for 30 days of storage at 60 °C, while the buffer-stored sample had already been deactivated. The microfluidic enzyme reactor was efficiently used for the trans-esterification of ethyl ferulate (EF) with glycerol towards the production of glyceryl ferulate (GF), known for its antioxidant potential. The biocatalytic process under continuous flow conditions exhibited 23 times higher productivity than the batch reaction system. This study featured an effective and robust biocatalytic system with immobilized lipase that can be used both in hydrolytic and synthetic applications, while further optimization is expected to upgrade the microreactor system performance. Article in Journal/Newspaper Antarc* Antarctica Directory of Open Access Journals: DOAJ Articles Micromachines 13 11 1954 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
3D printing immobilization enzyme microreactor deep eutectic solvents lipase Mechanical engineering and machinery TJ1-1570 |
spellingShingle |
3D printing immobilization enzyme microreactor deep eutectic solvents lipase Mechanical engineering and machinery TJ1-1570 Myrto G. Bellou Elena Gkantzou Anastasia Skonta Dimitrios Moschovas Konstantinos Spyrou Apostolos Avgeropoulos Dimitrios Gournis Haralambos Stamatis Development of 3D Printed Enzymatic Microreactors for Lipase-Catalyzed Reactions in Deep Eutectic Solvent-Based Media |
topic_facet |
3D printing immobilization enzyme microreactor deep eutectic solvents lipase Mechanical engineering and machinery TJ1-1570 |
description |
In this study, 3D printing technology was exploited for the development of immobilized enzyme microreactors that could be used for biocatalytic processes in Deep Eutectic Solvent (DES)-based media. 3D-printed polylactic acid (PLA) microwell plates or tubular microfluidic reactors were modified with polyethylenimine (PEI) and lipase from Candida antarctica (CALB) was covalently immobilized in the interior of each structure. DESs were found to have a negligible effect on the activity and stability of CALB, and the system proved highly stable and reusable in the presence of DESs for the hydrolysis of p-nitrophenyl butyrate (p-NPB). A kinetic study under flow conditions revealed an enhancement of substrate accessibility in the presence of Betaine: Glycerol (Bet:Gly) DES, while the system was not severely affected by diffusion limitations. Incubation of microreactors in 100% Bet:Gly preserved the enzyme activity by 53% for 30 days of storage at 60 °C, while the buffer-stored sample had already been deactivated. The microfluidic enzyme reactor was efficiently used for the trans-esterification of ethyl ferulate (EF) with glycerol towards the production of glyceryl ferulate (GF), known for its antioxidant potential. The biocatalytic process under continuous flow conditions exhibited 23 times higher productivity than the batch reaction system. This study featured an effective and robust biocatalytic system with immobilized lipase that can be used both in hydrolytic and synthetic applications, while further optimization is expected to upgrade the microreactor system performance. |
format |
Article in Journal/Newspaper |
author |
Myrto G. Bellou Elena Gkantzou Anastasia Skonta Dimitrios Moschovas Konstantinos Spyrou Apostolos Avgeropoulos Dimitrios Gournis Haralambos Stamatis |
author_facet |
Myrto G. Bellou Elena Gkantzou Anastasia Skonta Dimitrios Moschovas Konstantinos Spyrou Apostolos Avgeropoulos Dimitrios Gournis Haralambos Stamatis |
author_sort |
Myrto G. Bellou |
title |
Development of 3D Printed Enzymatic Microreactors for Lipase-Catalyzed Reactions in Deep Eutectic Solvent-Based Media |
title_short |
Development of 3D Printed Enzymatic Microreactors for Lipase-Catalyzed Reactions in Deep Eutectic Solvent-Based Media |
title_full |
Development of 3D Printed Enzymatic Microreactors for Lipase-Catalyzed Reactions in Deep Eutectic Solvent-Based Media |
title_fullStr |
Development of 3D Printed Enzymatic Microreactors for Lipase-Catalyzed Reactions in Deep Eutectic Solvent-Based Media |
title_full_unstemmed |
Development of 3D Printed Enzymatic Microreactors for Lipase-Catalyzed Reactions in Deep Eutectic Solvent-Based Media |
title_sort |
development of 3d printed enzymatic microreactors for lipase-catalyzed reactions in deep eutectic solvent-based media |
publisher |
MDPI AG |
publishDate |
2022 |
url |
https://doi.org/10.3390/mi13111954 https://doaj.org/article/3d54868b3a154dd98bb6f4f797d52448 |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_source |
Micromachines, Vol 13, Iss 1954, p 1954 (2022) |
op_relation |
https://www.mdpi.com/2072-666X/13/11/1954 https://doaj.org/toc/2072-666X doi:10.3390/mi13111954 2072-666X https://doaj.org/article/3d54868b3a154dd98bb6f4f797d52448 |
op_doi |
https://doi.org/10.3390/mi13111954 |
container_title |
Micromachines |
container_volume |
13 |
container_issue |
11 |
container_start_page |
1954 |
_version_ |
1766262637092405248 |