cagE as a biomarker of the pathogenicity of Helicobacter pylori
Introduction Helicobacter pylori infection is associated with gastro-duodenal diseases. Genes related to pathogenicity have been described for H. pylori and some of them appear to be associated with more severe clinical outcomes of the infection. The present study investigates the role of cagE as a...
Published in: | Revista da Sociedade Brasileira de Medicina Tropical |
---|---|
Main Authors: | , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Sociedade Brasileira de Medicina Tropical (SBMT)
2013
|
Subjects: | |
Online Access: | https://doi.org/10.1590/0037-8682-0054-2012 https://doaj.org/article/3d1d92c9c47e454383210d33b134a24b |
id |
ftdoajarticles:oai:doaj.org/article:3d1d92c9c47e454383210d33b134a24b |
---|---|
record_format |
openpolar |
spelling |
ftdoajarticles:oai:doaj.org/article:3d1d92c9c47e454383210d33b134a24b 2023-05-15T15:12:18+02:00 cagE as a biomarker of the pathogenicity of Helicobacter pylori Ivy Bastos Ramis Júlia Silveira Vianna Lande Vieira da Silva Junior Andrea Von Groll Pedro Eduardo Almeida da Silva 2013-04-01T00:00:00Z https://doi.org/10.1590/0037-8682-0054-2012 https://doaj.org/article/3d1d92c9c47e454383210d33b134a24b EN eng Sociedade Brasileira de Medicina Tropical (SBMT) http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822013000200185&lng=en&tlng=en https://doaj.org/toc/1678-9849 1678-9849 doi:10.1590/0037-8682-0054-2012 https://doaj.org/article/3d1d92c9c47e454383210d33b134a24b Revista da Sociedade Brasileira de Medicina Tropical, Vol 46, Iss 2, Pp 185-189 (2013) Helicobacter pylori Pathogenicity genes Endoscopic diagnosis Arctic medicine. Tropical medicine RC955-962 article 2013 ftdoajarticles https://doi.org/10.1590/0037-8682-0054-2012 2022-12-31T02:57:09Z Introduction Helicobacter pylori infection is associated with gastro-duodenal diseases. Genes related to pathogenicity have been described for H. pylori and some of them appear to be associated with more severe clinical outcomes of the infection. The present study investigates the role of cagE as a pathogenicity biomarker of H. pylori compare it to cagA, vacA, iceA and babA2 genes and correlate with endoscopic diagnoses. Methods Were collected biopsy samples of 144 dyspeptic patients at the Hospital of the Federal University of Rio Grande, Rio Grande do Sul, Brazil. After collection, the samples were sent for histological examination, DNA extraction and detection of all putative pathogenicity genes by PCR. Results Of the 144 patients undergoing endoscopy, 57 (39.6%) presented H. pylori by histological examination and PCR by detection of the ureA gene. Based on the endoscopic diagnoses, 45.6% (26/57) of the patients had erosive gastritis, while 54.4% (31/57) had enanthematous gastritis. The genes cagA, cagE, vacAs1/m1, vacAs1/m2 and iceA1 were related to erosive gastritis, while the genes vacAs2/m2, iceA2 and babA2 were associated to enanthematous gastritis. We found a statistically significant association between the presence of cagE and the endoscopic diagnosis. However, we detect no statistically significant association between the endoscopic diagnosis and the presence of cagA, vacA, iceA and babA2, although a biological association has been suggested. Conclusions Thus, cagE could be a risk biomarker for gastric lesions and may contribute to a better evaluation of the H. pylori pathogenic potential and to the prognosis of infection evolution in the gastric mucosa. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Revista da Sociedade Brasileira de Medicina Tropical 46 2 185 189 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Helicobacter pylori Pathogenicity genes Endoscopic diagnosis Arctic medicine. Tropical medicine RC955-962 |
spellingShingle |
Helicobacter pylori Pathogenicity genes Endoscopic diagnosis Arctic medicine. Tropical medicine RC955-962 Ivy Bastos Ramis Júlia Silveira Vianna Lande Vieira da Silva Junior Andrea Von Groll Pedro Eduardo Almeida da Silva cagE as a biomarker of the pathogenicity of Helicobacter pylori |
topic_facet |
Helicobacter pylori Pathogenicity genes Endoscopic diagnosis Arctic medicine. Tropical medicine RC955-962 |
description |
Introduction Helicobacter pylori infection is associated with gastro-duodenal diseases. Genes related to pathogenicity have been described for H. pylori and some of them appear to be associated with more severe clinical outcomes of the infection. The present study investigates the role of cagE as a pathogenicity biomarker of H. pylori compare it to cagA, vacA, iceA and babA2 genes and correlate with endoscopic diagnoses. Methods Were collected biopsy samples of 144 dyspeptic patients at the Hospital of the Federal University of Rio Grande, Rio Grande do Sul, Brazil. After collection, the samples were sent for histological examination, DNA extraction and detection of all putative pathogenicity genes by PCR. Results Of the 144 patients undergoing endoscopy, 57 (39.6%) presented H. pylori by histological examination and PCR by detection of the ureA gene. Based on the endoscopic diagnoses, 45.6% (26/57) of the patients had erosive gastritis, while 54.4% (31/57) had enanthematous gastritis. The genes cagA, cagE, vacAs1/m1, vacAs1/m2 and iceA1 were related to erosive gastritis, while the genes vacAs2/m2, iceA2 and babA2 were associated to enanthematous gastritis. We found a statistically significant association between the presence of cagE and the endoscopic diagnosis. However, we detect no statistically significant association between the endoscopic diagnosis and the presence of cagA, vacA, iceA and babA2, although a biological association has been suggested. Conclusions Thus, cagE could be a risk biomarker for gastric lesions and may contribute to a better evaluation of the H. pylori pathogenic potential and to the prognosis of infection evolution in the gastric mucosa. |
format |
Article in Journal/Newspaper |
author |
Ivy Bastos Ramis Júlia Silveira Vianna Lande Vieira da Silva Junior Andrea Von Groll Pedro Eduardo Almeida da Silva |
author_facet |
Ivy Bastos Ramis Júlia Silveira Vianna Lande Vieira da Silva Junior Andrea Von Groll Pedro Eduardo Almeida da Silva |
author_sort |
Ivy Bastos Ramis |
title |
cagE as a biomarker of the pathogenicity of Helicobacter pylori |
title_short |
cagE as a biomarker of the pathogenicity of Helicobacter pylori |
title_full |
cagE as a biomarker of the pathogenicity of Helicobacter pylori |
title_fullStr |
cagE as a biomarker of the pathogenicity of Helicobacter pylori |
title_full_unstemmed |
cagE as a biomarker of the pathogenicity of Helicobacter pylori |
title_sort |
cage as a biomarker of the pathogenicity of helicobacter pylori |
publisher |
Sociedade Brasileira de Medicina Tropical (SBMT) |
publishDate |
2013 |
url |
https://doi.org/10.1590/0037-8682-0054-2012 https://doaj.org/article/3d1d92c9c47e454383210d33b134a24b |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Revista da Sociedade Brasileira de Medicina Tropical, Vol 46, Iss 2, Pp 185-189 (2013) |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822013000200185&lng=en&tlng=en https://doaj.org/toc/1678-9849 1678-9849 doi:10.1590/0037-8682-0054-2012 https://doaj.org/article/3d1d92c9c47e454383210d33b134a24b |
op_doi |
https://doi.org/10.1590/0037-8682-0054-2012 |
container_title |
Revista da Sociedade Brasileira de Medicina Tropical |
container_volume |
46 |
container_issue |
2 |
container_start_page |
185 |
op_container_end_page |
189 |
_version_ |
1766342999090921472 |