Conus vexillum venom induces oxidative stress in Ehrlich's ascites carcinoma cells: an insight into the mechanism of induction
Background It is estimated that venoms of marine cone snails (genus Conus) contain more than 100,000 different small peptides with a wide range of pharmacological and biological actions. Some of these peptides were developed into potential therapeutic agents and as molecular tools to understand biol...
Published in: | Journal of Venomous Animals and Toxins including Tropical Diseases |
---|---|
Main Authors: | , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
SciELO
2013
|
Subjects: | |
Online Access: | https://doi.org/10.1186/1678-9199-19-10 https://doaj.org/article/3ceeb2011a6f4fc8be8a91f73efd6b32 |
id |
ftdoajarticles:oai:doaj.org/article:3ceeb2011a6f4fc8be8a91f73efd6b32 |
---|---|
record_format |
openpolar |
spelling |
ftdoajarticles:oai:doaj.org/article:3ceeb2011a6f4fc8be8a91f73efd6b32 2023-05-15T15:13:48+02:00 Conus vexillum venom induces oxidative stress in Ehrlich's ascites carcinoma cells: an insight into the mechanism of induction Mohamed A Abdel-Rahman Ismail M Abdel-Nabi Mohamed S El-Naggar Osama A Abbas Peter N Strong 2013-05-01T00:00:00Z https://doi.org/10.1186/1678-9199-19-10 https://doaj.org/article/3ceeb2011a6f4fc8be8a91f73efd6b32 EN eng SciELO http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992013000100307&lng=en&tlng=en https://doaj.org/toc/1678-9199 1678-9199 doi:10.1186/1678-9199-19-10 https://doaj.org/article/3ceeb2011a6f4fc8be8a91f73efd6b32 Journal of Venomous Animals and Toxins including Tropical Diseases, Vol 19, Iss 0 (2013) Conus vexillum venom Ehrlich’s cells Oxidative stress Cancer Egypt Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 article 2013 ftdoajarticles https://doi.org/10.1186/1678-9199-19-10 2022-12-31T12:29:50Z Background It is estimated that venoms of marine cone snails (genus Conus) contain more than 100,000 different small peptides with a wide range of pharmacological and biological actions. Some of these peptides were developed into potential therapeutic agents and as molecular tools to understand biological functions of nervous and cardiovascular systems. In this study we examined the cytotoxic and anticancer properties of the marine vermivorous cone snail Conus vexillum (collected from Hurgada and Sharm El-Shaikh, Red Sea, Egypt) and suggest the possible mechanisms involved. The in vitro cytotoxic effects of Conus venom were assessed against Ehrlich’s ascites carcinoma (EAC) cells. Results Conus venom treatment resulted in concentration-dependent cytotoxicity as indicated by a lactate dehydrogenase leakage assay. Apoptotic effects were measured in vivo by measuring levels of reactive oxygen species and oxidative defense agents in albino mice injected with EAC cells. Conus venom (1.25 mg/kg) induced a significant increase ( p < 0.05) in several oxidative stress biomarkers (lipid peroxidation, protein carbonyl content and reactive nitrogen intermediates) of EAC cells after 3, 6, 9 and 12 hours of venom injection. Conus venom significantly reduced ( p < 0.05) the activities of oxidative defense enzymes (catalase and superoxide dismutase) as well as the total antioxidant capacity of EAC cells, as evidenced by lowered levels of reduced glutathione. Conclusions These results demonstrate the cytotoxic potential of C. vexillum venom by inducing oxidative stress mediated mechanisms in tumor cells and suggest that the venom contains novel molecules with potential anticancer activity. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Journal of Venomous Animals and Toxins including Tropical Diseases 19 1 10 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Conus vexillum venom Ehrlich’s cells Oxidative stress Cancer Egypt Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 |
spellingShingle |
Conus vexillum venom Ehrlich’s cells Oxidative stress Cancer Egypt Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 Mohamed A Abdel-Rahman Ismail M Abdel-Nabi Mohamed S El-Naggar Osama A Abbas Peter N Strong Conus vexillum venom induces oxidative stress in Ehrlich's ascites carcinoma cells: an insight into the mechanism of induction |
topic_facet |
Conus vexillum venom Ehrlich’s cells Oxidative stress Cancer Egypt Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 |
description |
Background It is estimated that venoms of marine cone snails (genus Conus) contain more than 100,000 different small peptides with a wide range of pharmacological and biological actions. Some of these peptides were developed into potential therapeutic agents and as molecular tools to understand biological functions of nervous and cardiovascular systems. In this study we examined the cytotoxic and anticancer properties of the marine vermivorous cone snail Conus vexillum (collected from Hurgada and Sharm El-Shaikh, Red Sea, Egypt) and suggest the possible mechanisms involved. The in vitro cytotoxic effects of Conus venom were assessed against Ehrlich’s ascites carcinoma (EAC) cells. Results Conus venom treatment resulted in concentration-dependent cytotoxicity as indicated by a lactate dehydrogenase leakage assay. Apoptotic effects were measured in vivo by measuring levels of reactive oxygen species and oxidative defense agents in albino mice injected with EAC cells. Conus venom (1.25 mg/kg) induced a significant increase ( p < 0.05) in several oxidative stress biomarkers (lipid peroxidation, protein carbonyl content and reactive nitrogen intermediates) of EAC cells after 3, 6, 9 and 12 hours of venom injection. Conus venom significantly reduced ( p < 0.05) the activities of oxidative defense enzymes (catalase and superoxide dismutase) as well as the total antioxidant capacity of EAC cells, as evidenced by lowered levels of reduced glutathione. Conclusions These results demonstrate the cytotoxic potential of C. vexillum venom by inducing oxidative stress mediated mechanisms in tumor cells and suggest that the venom contains novel molecules with potential anticancer activity. |
format |
Article in Journal/Newspaper |
author |
Mohamed A Abdel-Rahman Ismail M Abdel-Nabi Mohamed S El-Naggar Osama A Abbas Peter N Strong |
author_facet |
Mohamed A Abdel-Rahman Ismail M Abdel-Nabi Mohamed S El-Naggar Osama A Abbas Peter N Strong |
author_sort |
Mohamed A Abdel-Rahman |
title |
Conus vexillum venom induces oxidative stress in Ehrlich's ascites carcinoma cells: an insight into the mechanism of induction |
title_short |
Conus vexillum venom induces oxidative stress in Ehrlich's ascites carcinoma cells: an insight into the mechanism of induction |
title_full |
Conus vexillum venom induces oxidative stress in Ehrlich's ascites carcinoma cells: an insight into the mechanism of induction |
title_fullStr |
Conus vexillum venom induces oxidative stress in Ehrlich's ascites carcinoma cells: an insight into the mechanism of induction |
title_full_unstemmed |
Conus vexillum venom induces oxidative stress in Ehrlich's ascites carcinoma cells: an insight into the mechanism of induction |
title_sort |
conus vexillum venom induces oxidative stress in ehrlich's ascites carcinoma cells: an insight into the mechanism of induction |
publisher |
SciELO |
publishDate |
2013 |
url |
https://doi.org/10.1186/1678-9199-19-10 https://doaj.org/article/3ceeb2011a6f4fc8be8a91f73efd6b32 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Journal of Venomous Animals and Toxins including Tropical Diseases, Vol 19, Iss 0 (2013) |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992013000100307&lng=en&tlng=en https://doaj.org/toc/1678-9199 1678-9199 doi:10.1186/1678-9199-19-10 https://doaj.org/article/3ceeb2011a6f4fc8be8a91f73efd6b32 |
op_doi |
https://doi.org/10.1186/1678-9199-19-10 |
container_title |
Journal of Venomous Animals and Toxins including Tropical Diseases |
container_volume |
19 |
container_issue |
1 |
container_start_page |
10 |
_version_ |
1766344326360596480 |