Bitter gourd extract improves glucose homeostasis and lipid profile via enhancing insulin signaling in the liver and skeletal muscles of diabetic rats
Objective: To investigate the modulatory effects of bitter gourd extract on the insulin signaling pathway in the liver and skeletal muscle tissues of diabetic rats. Methods: The ethanolic extract of bitter gourd was prepared and its contents of total polyphenols and flavonoids were assayed. A neonat...
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Wolters Kluwer Medknow Publications
2021
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ftdoajarticles:oai:doaj.org/article:3cd2ca0a6eb84455935fad95eec72fda 2023-05-15T15:15:56+02:00 Bitter gourd extract improves glucose homeostasis and lipid profile via enhancing insulin signaling in the liver and skeletal muscles of diabetic rats Saber Mohamed Eweda Mennatallah Ahmed Ali Hala Mohamed Abd El-Bary Nahed Hussein El-Sokkary Madiha Hassan Helmy Maher Abdel-Nabi Kamel 2021-01-01T00:00:00Z https://doi.org/10.4103/2221-1691.319569 https://doaj.org/article/3cd2ca0a6eb84455935fad95eec72fda EN eng Wolters Kluwer Medknow Publications http://www.apjtb.org/article.asp?issn=2221-1691;year=2021;volume=11;issue=8;spage=344;epage=352;aulast=Eweda https://doaj.org/toc/2221-1691 https://doaj.org/toc/2588-9222 2221-1691 2588-9222 doi:10.4103/2221-1691.319569 https://doaj.org/article/3cd2ca0a6eb84455935fad95eec72fda Asian Pacific Journal of Tropical Biomedicine, Vol 11, Iss 8, Pp 344-352 (2021) neonatal streptozotocin diabetic sulfonylurea bitter gourd phosphorylated insulin receptor protein kinase c irs-1 glut2/glut4 insulin signaling Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 article 2021 ftdoajarticles https://doi.org/10.4103/2221-1691.319569 2022-12-31T00:28:41Z Objective: To investigate the modulatory effects of bitter gourd extract on the insulin signaling pathway in the liver and skeletal muscle tissues of diabetic rats. Methods: The ethanolic extract of bitter gourd was prepared and its contents of total polyphenols and flavonoids were assayed. A neonatal streptozotocin-induced diabetic rat model was established and the diabetic rats were assigned into different groups and were treated with different doses of bitter gourd extract (100, 200, 400, or 600 mg/kg) or with glibenclamide (0.1 mg/kg) for 30 d. Fasting blood glucose, insulin, and lipid profile were evaluated and the insulin signaling pathway in the liver and skeletal muscle of rats was investigated. The correlations between homeostasis model assessment (HOMA) and the components of insulin signaling pathway were also evaluated. Results: Different doses of bitter gourd extract significantly ameliorated fasting blood glucose level and HOMA index for insulin resistance. Moreover, bitter gourd extract increased serum insulin and improved disrupted serum lipid profile. The levels of insulin receptor substrate-1 (IRS-1), p-insulin receptor β (p-IR-β), protein kinase C (PKC), GLUT2, and GLUT4 were improved by treatment with bitter gourd extract. The best results were obtained with 400 mg/kg dose of the extract, the effect of which was equivalent to that of glibenclamide. HOMA in the bitter gourd treated rats was negatively correlated with p-IR-β, IRS-1 and PKC in hepatic and skeletal muscle. HOMA was also negatively correlated with skeletal muscle GLUT4. Conclusions: Bitter gourd extract improves glucose homeostasis and lipid profile in diabetic rats via enhancement of insulin secretion and sensitivity. Therefore, bitter gourd can be used as a potential pharmacological agent for the treatment of type 2 diabetes mellitus. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Asian Pacific Journal of Tropical Biomedicine 11 8 344 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English |
topic |
neonatal streptozotocin diabetic sulfonylurea bitter gourd phosphorylated insulin receptor protein kinase c irs-1 glut2/glut4 insulin signaling Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 |
spellingShingle |
neonatal streptozotocin diabetic sulfonylurea bitter gourd phosphorylated insulin receptor protein kinase c irs-1 glut2/glut4 insulin signaling Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 Saber Mohamed Eweda Mennatallah Ahmed Ali Hala Mohamed Abd El-Bary Nahed Hussein El-Sokkary Madiha Hassan Helmy Maher Abdel-Nabi Kamel Bitter gourd extract improves glucose homeostasis and lipid profile via enhancing insulin signaling in the liver and skeletal muscles of diabetic rats |
topic_facet |
neonatal streptozotocin diabetic sulfonylurea bitter gourd phosphorylated insulin receptor protein kinase c irs-1 glut2/glut4 insulin signaling Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 |
description |
Objective: To investigate the modulatory effects of bitter gourd extract on the insulin signaling pathway in the liver and skeletal muscle tissues of diabetic rats. Methods: The ethanolic extract of bitter gourd was prepared and its contents of total polyphenols and flavonoids were assayed. A neonatal streptozotocin-induced diabetic rat model was established and the diabetic rats were assigned into different groups and were treated with different doses of bitter gourd extract (100, 200, 400, or 600 mg/kg) or with glibenclamide (0.1 mg/kg) for 30 d. Fasting blood glucose, insulin, and lipid profile were evaluated and the insulin signaling pathway in the liver and skeletal muscle of rats was investigated. The correlations between homeostasis model assessment (HOMA) and the components of insulin signaling pathway were also evaluated. Results: Different doses of bitter gourd extract significantly ameliorated fasting blood glucose level and HOMA index for insulin resistance. Moreover, bitter gourd extract increased serum insulin and improved disrupted serum lipid profile. The levels of insulin receptor substrate-1 (IRS-1), p-insulin receptor β (p-IR-β), protein kinase C (PKC), GLUT2, and GLUT4 were improved by treatment with bitter gourd extract. The best results were obtained with 400 mg/kg dose of the extract, the effect of which was equivalent to that of glibenclamide. HOMA in the bitter gourd treated rats was negatively correlated with p-IR-β, IRS-1 and PKC in hepatic and skeletal muscle. HOMA was also negatively correlated with skeletal muscle GLUT4. Conclusions: Bitter gourd extract improves glucose homeostasis and lipid profile in diabetic rats via enhancement of insulin secretion and sensitivity. Therefore, bitter gourd can be used as a potential pharmacological agent for the treatment of type 2 diabetes mellitus. |
format |
Article in Journal/Newspaper |
author |
Saber Mohamed Eweda Mennatallah Ahmed Ali Hala Mohamed Abd El-Bary Nahed Hussein El-Sokkary Madiha Hassan Helmy Maher Abdel-Nabi Kamel |
author_facet |
Saber Mohamed Eweda Mennatallah Ahmed Ali Hala Mohamed Abd El-Bary Nahed Hussein El-Sokkary Madiha Hassan Helmy Maher Abdel-Nabi Kamel |
author_sort |
Saber Mohamed Eweda |
title |
Bitter gourd extract improves glucose homeostasis and lipid profile via enhancing insulin signaling in the liver and skeletal muscles of diabetic rats |
title_short |
Bitter gourd extract improves glucose homeostasis and lipid profile via enhancing insulin signaling in the liver and skeletal muscles of diabetic rats |
title_full |
Bitter gourd extract improves glucose homeostasis and lipid profile via enhancing insulin signaling in the liver and skeletal muscles of diabetic rats |
title_fullStr |
Bitter gourd extract improves glucose homeostasis and lipid profile via enhancing insulin signaling in the liver and skeletal muscles of diabetic rats |
title_full_unstemmed |
Bitter gourd extract improves glucose homeostasis and lipid profile via enhancing insulin signaling in the liver and skeletal muscles of diabetic rats |
title_sort |
bitter gourd extract improves glucose homeostasis and lipid profile via enhancing insulin signaling in the liver and skeletal muscles of diabetic rats |
publisher |
Wolters Kluwer Medknow Publications |
publishDate |
2021 |
url |
https://doi.org/10.4103/2221-1691.319569 https://doaj.org/article/3cd2ca0a6eb84455935fad95eec72fda |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Asian Pacific Journal of Tropical Biomedicine, Vol 11, Iss 8, Pp 344-352 (2021) |
op_relation |
http://www.apjtb.org/article.asp?issn=2221-1691;year=2021;volume=11;issue=8;spage=344;epage=352;aulast=Eweda https://doaj.org/toc/2221-1691 https://doaj.org/toc/2588-9222 2221-1691 2588-9222 doi:10.4103/2221-1691.319569 https://doaj.org/article/3cd2ca0a6eb84455935fad95eec72fda |
op_doi |
https://doi.org/10.4103/2221-1691.319569 |
container_title |
Asian Pacific Journal of Tropical Biomedicine |
container_volume |
11 |
container_issue |
8 |
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344 |
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1766346266081492992 |