Severe South American ocular toxoplasmosis is associated with decreased Ifn-γ/Il-17a and increased Il-6/Il-13 intraocular levels.
In a cross sectional study, 19 French and 23 Colombian cases of confirmed active ocular toxoplasmosis (OT) were evaluated. The objective was to compare clinical, parasitological and immunological responses and relate them to the infecting strains. A complete ocular examination was performed in each...
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ftdoajarticles:oai:doaj.org/article:3cb184ea2d72493781383585c07fe05c 2023-05-15T15:09:18+02:00 Severe South American ocular toxoplasmosis is associated with decreased Ifn-γ/Il-17a and increased Il-6/Il-13 intraocular levels. Alejandra de-la-Torre Arnaud Sauer Alexander W Pfaff Tristan Bourcier Julie Brunet Claude Speeg-Schatz Laurent Ballonzoli Odile Villard Daniel Ajzenberg Natarajan Sundar Michael E Grigg Jorge E Gomez-Marin Ermanno Candolfi 2013-11-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0002541 https://doaj.org/article/3cb184ea2d72493781383585c07fe05c EN eng Public Library of Science (PLoS) https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24278490/?tool=EBI https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0002541 https://doaj.org/article/3cb184ea2d72493781383585c07fe05c PLoS Neglected Tropical Diseases, Vol 7, Iss 11, p e2541 (2013) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2013 ftdoajarticles https://doi.org/10.1371/journal.pntd.0002541 2022-12-31T05:48:02Z In a cross sectional study, 19 French and 23 Colombian cases of confirmed active ocular toxoplasmosis (OT) were evaluated. The objective was to compare clinical, parasitological and immunological responses and relate them to the infecting strains. A complete ocular examination was performed in each patient. The infecting strain was characterized by genotyping when intraocular Toxoplasma DNA was detectable, as well as by peptide-specific serotyping for each patient. To characterize the immune response, we assessed Toxoplasma protein recognition patterns by intraocular antibodies and the intraocular profile of cytokines, chemokines and growth factors. Significant differences were found for size of active lesions, unilateral macular involvement, unilateral visual impairment, vitreous inflammation, synechiae, and vasculitis, with higher values observed throughout for Colombian patients. Multilocus PCR-DNA sequence genotyping was only successful in three Colombian patients revealing one type I and two atypical strains. The Colombian OT patients possessed heterogeneous atypical serotypes whereas the French were uniformly reactive to type II strain peptides. The protein patterns recognized by intraocular antibodies and the cytokine patterns were strikingly different between the two populations. Intraocular IFN-γ and IL-17 expression was lower, while higher levels of IL-13 and IL-6 were detected in aqueous humor of Colombian patients. Our results are consistent with the hypothesis that South American strains may cause more severe OT due to an inhibition of the protective effect of IFN-γ. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLoS Neglected Tropical Diseases 7 11 e2541 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
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English |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Alejandra de-la-Torre Arnaud Sauer Alexander W Pfaff Tristan Bourcier Julie Brunet Claude Speeg-Schatz Laurent Ballonzoli Odile Villard Daniel Ajzenberg Natarajan Sundar Michael E Grigg Jorge E Gomez-Marin Ermanno Candolfi Severe South American ocular toxoplasmosis is associated with decreased Ifn-γ/Il-17a and increased Il-6/Il-13 intraocular levels. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
In a cross sectional study, 19 French and 23 Colombian cases of confirmed active ocular toxoplasmosis (OT) were evaluated. The objective was to compare clinical, parasitological and immunological responses and relate them to the infecting strains. A complete ocular examination was performed in each patient. The infecting strain was characterized by genotyping when intraocular Toxoplasma DNA was detectable, as well as by peptide-specific serotyping for each patient. To characterize the immune response, we assessed Toxoplasma protein recognition patterns by intraocular antibodies and the intraocular profile of cytokines, chemokines and growth factors. Significant differences were found for size of active lesions, unilateral macular involvement, unilateral visual impairment, vitreous inflammation, synechiae, and vasculitis, with higher values observed throughout for Colombian patients. Multilocus PCR-DNA sequence genotyping was only successful in three Colombian patients revealing one type I and two atypical strains. The Colombian OT patients possessed heterogeneous atypical serotypes whereas the French were uniformly reactive to type II strain peptides. The protein patterns recognized by intraocular antibodies and the cytokine patterns were strikingly different between the two populations. Intraocular IFN-γ and IL-17 expression was lower, while higher levels of IL-13 and IL-6 were detected in aqueous humor of Colombian patients. Our results are consistent with the hypothesis that South American strains may cause more severe OT due to an inhibition of the protective effect of IFN-γ. |
format |
Article in Journal/Newspaper |
author |
Alejandra de-la-Torre Arnaud Sauer Alexander W Pfaff Tristan Bourcier Julie Brunet Claude Speeg-Schatz Laurent Ballonzoli Odile Villard Daniel Ajzenberg Natarajan Sundar Michael E Grigg Jorge E Gomez-Marin Ermanno Candolfi |
author_facet |
Alejandra de-la-Torre Arnaud Sauer Alexander W Pfaff Tristan Bourcier Julie Brunet Claude Speeg-Schatz Laurent Ballonzoli Odile Villard Daniel Ajzenberg Natarajan Sundar Michael E Grigg Jorge E Gomez-Marin Ermanno Candolfi |
author_sort |
Alejandra de-la-Torre |
title |
Severe South American ocular toxoplasmosis is associated with decreased Ifn-γ/Il-17a and increased Il-6/Il-13 intraocular levels. |
title_short |
Severe South American ocular toxoplasmosis is associated with decreased Ifn-γ/Il-17a and increased Il-6/Il-13 intraocular levels. |
title_full |
Severe South American ocular toxoplasmosis is associated with decreased Ifn-γ/Il-17a and increased Il-6/Il-13 intraocular levels. |
title_fullStr |
Severe South American ocular toxoplasmosis is associated with decreased Ifn-γ/Il-17a and increased Il-6/Il-13 intraocular levels. |
title_full_unstemmed |
Severe South American ocular toxoplasmosis is associated with decreased Ifn-γ/Il-17a and increased Il-6/Il-13 intraocular levels. |
title_sort |
severe south american ocular toxoplasmosis is associated with decreased ifn-γ/il-17a and increased il-6/il-13 intraocular levels. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2013 |
url |
https://doi.org/10.1371/journal.pntd.0002541 https://doaj.org/article/3cb184ea2d72493781383585c07fe05c |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 7, Iss 11, p e2541 (2013) |
op_relation |
https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24278490/?tool=EBI https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0002541 https://doaj.org/article/3cb184ea2d72493781383585c07fe05c |
op_doi |
https://doi.org/10.1371/journal.pntd.0002541 |
container_title |
PLoS Neglected Tropical Diseases |
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7 |
container_issue |
11 |
container_start_page |
e2541 |
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