Induction of cell migration and activation in mice by the freshwater sponge Drulia uruguayensis Bonetto & Ezcurra de Drago, 1968 (Porifera: Metaniidae)
Freshwater sponges are abundant in the Amazon region and they have been known to cause dermatitis (acute inflammation) since the beginning of the 20th century. To determine whether additional constituents, besides their body spicules, cause dermatological reactions in humans, an experimental study w...
Published in: | Journal of Venomous Animals and Toxins including Tropical Diseases |
---|---|
Main Authors: | , , , , , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
SciELO
2011
|
Subjects: | |
Online Access: | https://doi.org/10.1590/S1678-91992011000100009 https://doaj.org/article/3c93d0196db34794b59c17f084d0ba2d |
id |
ftdoajarticles:oai:doaj.org/article:3c93d0196db34794b59c17f084d0ba2d |
---|---|
record_format |
openpolar |
spelling |
ftdoajarticles:oai:doaj.org/article:3c93d0196db34794b59c17f084d0ba2d 2023-05-15T15:10:31+02:00 Induction of cell migration and activation in mice by the freshwater sponge Drulia uruguayensis Bonetto & Ezcurra de Drago, 1968 (Porifera: Metaniidae) AO Magalhães C Volkmer-Ribeiro LBM Fujimoto MF Barbosa JL Cardoso JFM Barcellos CC Silva RR Campos MCF Cunha AP Freitas-Lemos MC Dos-Santos 2011-01-01T00:00:00Z https://doi.org/10.1590/S1678-91992011000100009 https://doaj.org/article/3c93d0196db34794b59c17f084d0ba2d EN eng SciELO http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992011000100009 https://doaj.org/toc/1678-9199 doi:10.1590/S1678-91992011000100009 1678-9199 https://doaj.org/article/3c93d0196db34794b59c17f084d0ba2d Journal of Venomous Animals and Toxins including Tropical Diseases, Vol 17, Iss 1, Pp 66-73 (2011) dermatitis Amazonia cauixi freshwater sponges Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 article 2011 ftdoajarticles https://doi.org/10.1590/S1678-91992011000100009 2022-12-31T04:02:16Z Freshwater sponges are abundant in the Amazon region and they have been known to cause dermatitis (acute inflammation) since the beginning of the 20th century. To determine whether additional constituents, besides their body spicules, cause dermatological reactions in humans, an experimental study was developed and carried out using mice and Drulia uruguayensis prepared in three different forms: intact sponges (IS), macerated sponges (MS) or isolated spicules - megascleres (ISM). The cells most commonly involved in inflammatory reactions (mast cells, eosinophils and neutrophils), as well as intraepithelial lymphocytes and degranulated mast cells, were counted so that they could be used as parameters to determine which of the sponge preparations induced the greatest reaction. The effects of the sponge on the skin were then determined by histological analysis. The results obtained showed that IS caused the greatest inflammatory reaction (p = 0.000005), activating mainly mast cells (p = 0.0018). The histopathological analysis revealed a slight loss of continuity of the epidermis when ISM or IS were applied. These findings allow us to conclude that a structurally intact sponge can cause a greater inflammatory reaction in the first contact because of its ability to perforate the skin and allow inflammatory agents to enter. Other proteins present in dried sponge bodies could induce allergic but not toxic responses (in contact with the entire sponge, a large number of pharmacologically inert proteins may be introduced, with a potential allergen). Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Ezcurra ENVELOPE(-58.535,-58.535,-62.162,-62.162) Drago ENVELOPE(-61.767,-61.767,-64.133,-64.133) Journal of Venomous Animals and Toxins including Tropical Diseases 17 1 66 73 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
dermatitis Amazonia cauixi freshwater sponges Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 |
spellingShingle |
dermatitis Amazonia cauixi freshwater sponges Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 AO Magalhães C Volkmer-Ribeiro LBM Fujimoto MF Barbosa JL Cardoso JFM Barcellos CC Silva RR Campos MCF Cunha AP Freitas-Lemos MC Dos-Santos Induction of cell migration and activation in mice by the freshwater sponge Drulia uruguayensis Bonetto & Ezcurra de Drago, 1968 (Porifera: Metaniidae) |
topic_facet |
dermatitis Amazonia cauixi freshwater sponges Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 |
description |
Freshwater sponges are abundant in the Amazon region and they have been known to cause dermatitis (acute inflammation) since the beginning of the 20th century. To determine whether additional constituents, besides their body spicules, cause dermatological reactions in humans, an experimental study was developed and carried out using mice and Drulia uruguayensis prepared in three different forms: intact sponges (IS), macerated sponges (MS) or isolated spicules - megascleres (ISM). The cells most commonly involved in inflammatory reactions (mast cells, eosinophils and neutrophils), as well as intraepithelial lymphocytes and degranulated mast cells, were counted so that they could be used as parameters to determine which of the sponge preparations induced the greatest reaction. The effects of the sponge on the skin were then determined by histological analysis. The results obtained showed that IS caused the greatest inflammatory reaction (p = 0.000005), activating mainly mast cells (p = 0.0018). The histopathological analysis revealed a slight loss of continuity of the epidermis when ISM or IS were applied. These findings allow us to conclude that a structurally intact sponge can cause a greater inflammatory reaction in the first contact because of its ability to perforate the skin and allow inflammatory agents to enter. Other proteins present in dried sponge bodies could induce allergic but not toxic responses (in contact with the entire sponge, a large number of pharmacologically inert proteins may be introduced, with a potential allergen). |
format |
Article in Journal/Newspaper |
author |
AO Magalhães C Volkmer-Ribeiro LBM Fujimoto MF Barbosa JL Cardoso JFM Barcellos CC Silva RR Campos MCF Cunha AP Freitas-Lemos MC Dos-Santos |
author_facet |
AO Magalhães C Volkmer-Ribeiro LBM Fujimoto MF Barbosa JL Cardoso JFM Barcellos CC Silva RR Campos MCF Cunha AP Freitas-Lemos MC Dos-Santos |
author_sort |
AO Magalhães |
title |
Induction of cell migration and activation in mice by the freshwater sponge Drulia uruguayensis Bonetto & Ezcurra de Drago, 1968 (Porifera: Metaniidae) |
title_short |
Induction of cell migration and activation in mice by the freshwater sponge Drulia uruguayensis Bonetto & Ezcurra de Drago, 1968 (Porifera: Metaniidae) |
title_full |
Induction of cell migration and activation in mice by the freshwater sponge Drulia uruguayensis Bonetto & Ezcurra de Drago, 1968 (Porifera: Metaniidae) |
title_fullStr |
Induction of cell migration and activation in mice by the freshwater sponge Drulia uruguayensis Bonetto & Ezcurra de Drago, 1968 (Porifera: Metaniidae) |
title_full_unstemmed |
Induction of cell migration and activation in mice by the freshwater sponge Drulia uruguayensis Bonetto & Ezcurra de Drago, 1968 (Porifera: Metaniidae) |
title_sort |
induction of cell migration and activation in mice by the freshwater sponge drulia uruguayensis bonetto & ezcurra de drago, 1968 (porifera: metaniidae) |
publisher |
SciELO |
publishDate |
2011 |
url |
https://doi.org/10.1590/S1678-91992011000100009 https://doaj.org/article/3c93d0196db34794b59c17f084d0ba2d |
long_lat |
ENVELOPE(-58.535,-58.535,-62.162,-62.162) ENVELOPE(-61.767,-61.767,-64.133,-64.133) |
geographic |
Arctic Ezcurra Drago |
geographic_facet |
Arctic Ezcurra Drago |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Journal of Venomous Animals and Toxins including Tropical Diseases, Vol 17, Iss 1, Pp 66-73 (2011) |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992011000100009 https://doaj.org/toc/1678-9199 doi:10.1590/S1678-91992011000100009 1678-9199 https://doaj.org/article/3c93d0196db34794b59c17f084d0ba2d |
op_doi |
https://doi.org/10.1590/S1678-91992011000100009 |
container_title |
Journal of Venomous Animals and Toxins including Tropical Diseases |
container_volume |
17 |
container_issue |
1 |
container_start_page |
66 |
op_container_end_page |
73 |
_version_ |
1766341537063501824 |