Human milk extracellular vesicle miRNA expression and associations with maternal characteristics in a population-based cohort from the Faroe Islands
Abstract Human milk plays a critical role in infant development and health, particularly in cognitive, immune, and cardiometabolic functions. Milk contains extracellular vesicles (EVs) that can transport biologically relevant cargo from mother to infant, including microRNAs (miRNAs). We aimed to cha...
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ftdoajarticles:oai:doaj.org/article:3c3110bf9770408fa6bbe24d05b2019c 2023-05-15T16:10:39+02:00 Human milk extracellular vesicle miRNA expression and associations with maternal characteristics in a population-based cohort from the Faroe Islands Allison Kupsco Diddier Prada Damaskini Valvi Lisa Hu Maria Skaalum Petersen Brent Coull Philippe Grandjean Pal Weihe Andrea A. Baccarelli 2021-03-01T00:00:00Z https://doi.org/10.1038/s41598-021-84809-2 https://doaj.org/article/3c3110bf9770408fa6bbe24d05b2019c EN eng Nature Portfolio https://doi.org/10.1038/s41598-021-84809-2 https://doaj.org/toc/2045-2322 doi:10.1038/s41598-021-84809-2 2045-2322 https://doaj.org/article/3c3110bf9770408fa6bbe24d05b2019c Scientific Reports, Vol 11, Iss 1, Pp 1-17 (2021) Medicine R Science Q article 2021 ftdoajarticles https://doi.org/10.1038/s41598-021-84809-2 2022-12-31T07:11:24Z Abstract Human milk plays a critical role in infant development and health, particularly in cognitive, immune, and cardiometabolic functions. Milk contains extracellular vesicles (EVs) that can transport biologically relevant cargo from mother to infant, including microRNAs (miRNAs). We aimed to characterize milk EV-miRNA profiles in a human population cohort, assess potential pathways and ontology, and investigate associations with maternal characteristics. We conducted the first study to describe the EV miRNA profile of human milk in 364 mothers from a population-based mother-infant cohort in the Faroe Islands using small RNA sequencing. We detected 1523 miRNAs with ≥ one read in 70% of samples. Using hierarchical clustering, we determined five EV-miRNA clusters, the top three consisting of 15, 27 and 67 miRNAs. Correlation coefficients indicated that the expression of many miRNAs within the top three clusters was highly correlated. Top-cluster human milk EV-miRNAs were involved in pathways enriched for the endocrine system, cellular community, neurodevelopment, and cancers. miRNA expression was associated with time to milk collection post-delivery, maternal body mass index, and maternal smoking, but not maternal parity. Future studies investigating determinants of human EV-miRNAs and associated health outcomes are needed to elucidate the role of human milk EV-miRNAs in health and disease. Article in Journal/Newspaper Faroe Islands Directory of Open Access Journals: DOAJ Articles Faroe Islands Scientific Reports 11 1 |
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Directory of Open Access Journals: DOAJ Articles |
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English |
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Medicine R Science Q |
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Medicine R Science Q Allison Kupsco Diddier Prada Damaskini Valvi Lisa Hu Maria Skaalum Petersen Brent Coull Philippe Grandjean Pal Weihe Andrea A. Baccarelli Human milk extracellular vesicle miRNA expression and associations with maternal characteristics in a population-based cohort from the Faroe Islands |
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Medicine R Science Q |
description |
Abstract Human milk plays a critical role in infant development and health, particularly in cognitive, immune, and cardiometabolic functions. Milk contains extracellular vesicles (EVs) that can transport biologically relevant cargo from mother to infant, including microRNAs (miRNAs). We aimed to characterize milk EV-miRNA profiles in a human population cohort, assess potential pathways and ontology, and investigate associations with maternal characteristics. We conducted the first study to describe the EV miRNA profile of human milk in 364 mothers from a population-based mother-infant cohort in the Faroe Islands using small RNA sequencing. We detected 1523 miRNAs with ≥ one read in 70% of samples. Using hierarchical clustering, we determined five EV-miRNA clusters, the top three consisting of 15, 27 and 67 miRNAs. Correlation coefficients indicated that the expression of many miRNAs within the top three clusters was highly correlated. Top-cluster human milk EV-miRNAs were involved in pathways enriched for the endocrine system, cellular community, neurodevelopment, and cancers. miRNA expression was associated with time to milk collection post-delivery, maternal body mass index, and maternal smoking, but not maternal parity. Future studies investigating determinants of human EV-miRNAs and associated health outcomes are needed to elucidate the role of human milk EV-miRNAs in health and disease. |
format |
Article in Journal/Newspaper |
author |
Allison Kupsco Diddier Prada Damaskini Valvi Lisa Hu Maria Skaalum Petersen Brent Coull Philippe Grandjean Pal Weihe Andrea A. Baccarelli |
author_facet |
Allison Kupsco Diddier Prada Damaskini Valvi Lisa Hu Maria Skaalum Petersen Brent Coull Philippe Grandjean Pal Weihe Andrea A. Baccarelli |
author_sort |
Allison Kupsco |
title |
Human milk extracellular vesicle miRNA expression and associations with maternal characteristics in a population-based cohort from the Faroe Islands |
title_short |
Human milk extracellular vesicle miRNA expression and associations with maternal characteristics in a population-based cohort from the Faroe Islands |
title_full |
Human milk extracellular vesicle miRNA expression and associations with maternal characteristics in a population-based cohort from the Faroe Islands |
title_fullStr |
Human milk extracellular vesicle miRNA expression and associations with maternal characteristics in a population-based cohort from the Faroe Islands |
title_full_unstemmed |
Human milk extracellular vesicle miRNA expression and associations with maternal characteristics in a population-based cohort from the Faroe Islands |
title_sort |
human milk extracellular vesicle mirna expression and associations with maternal characteristics in a population-based cohort from the faroe islands |
publisher |
Nature Portfolio |
publishDate |
2021 |
url |
https://doi.org/10.1038/s41598-021-84809-2 https://doaj.org/article/3c3110bf9770408fa6bbe24d05b2019c |
geographic |
Faroe Islands |
geographic_facet |
Faroe Islands |
genre |
Faroe Islands |
genre_facet |
Faroe Islands |
op_source |
Scientific Reports, Vol 11, Iss 1, Pp 1-17 (2021) |
op_relation |
https://doi.org/10.1038/s41598-021-84809-2 https://doaj.org/toc/2045-2322 doi:10.1038/s41598-021-84809-2 2045-2322 https://doaj.org/article/3c3110bf9770408fa6bbe24d05b2019c |
op_doi |
https://doi.org/10.1038/s41598-021-84809-2 |
container_title |
Scientific Reports |
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11 |
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1 |
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1765995816178155520 |