Antivenomics as a tool to improve the neutralizing capacity of the crotalic antivenom: a study with crotamine
Abstract Background Snakebite treatment requires administration of an appropriate antivenom that should contain antibodies capable of neutralizing the venom. To achieve this goal, antivenom production must start from a suitable immunization protocol and proper venom mixtures. In Brazil, antivenom ag...
Published in: | Journal of Venomous Animals and Toxins including Tropical Diseases |
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ftdoajarticles:oai:doaj.org/article:3c21cf9986944e58888cfe311972b3a8 2023-05-15T15:11:30+02:00 Antivenomics as a tool to improve the neutralizing capacity of the crotalic antivenom: a study with crotamine Ricardo Teixeira-Araújo Patrícia Castanheira Leonora Brazil-Más Francisco Pontes Moema Leitão de Araújo Maria Lucia Machado Alves Russolina Benedeta Zingali Carlos Correa-Netto 2017-05-01T00:00:00Z https://doi.org/10.1186/s40409-017-0118-7 https://doaj.org/article/3c21cf9986944e58888cfe311972b3a8 EN eng SciELO http://link.springer.com/article/10.1186/s40409-017-0118-7 https://doaj.org/toc/1678-9199 doi:10.1186/s40409-017-0118-7 1678-9199 https://doaj.org/article/3c21cf9986944e58888cfe311972b3a8 Journal of Venomous Animals and Toxins including Tropical Diseases, Vol 23, Iss 1, Pp 1-8 (2017) Antivenom production Antivenomics Crotalus durissus Crotamine Crotoxin Geographic venom variation Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 article 2017 ftdoajarticles https://doi.org/10.1186/s40409-017-0118-7 2022-12-31T14:00:25Z Abstract Background Snakebite treatment requires administration of an appropriate antivenom that should contain antibodies capable of neutralizing the venom. To achieve this goal, antivenom production must start from a suitable immunization protocol and proper venom mixtures. In Brazil, antivenom against South American rattlesnake (Crotalus durissus terrificus) bites is produced by public institutions based on the guidelines defined by the regulatory agency of the Brazilian Ministry of Health, ANVISA. However, each institution uses its own mixture of rattlesnake venom antigens. Previous works have shown that crotamine, a toxin found in Crolatus durissus venom, shows marked individual and populational variation. In addition, serum produced from crotamine-negative venoms fails to recognize this molecule. Methods In this work, we used an antivenomics approach to assess the cross-reactivity of crotalic antivenom manufactured by IVB towards crotamine-negative venom and a mixture of crotamine-negative/crotamine-positive venoms. Results We show that the venom mixture containing 20% crotamine and 57% crotoxin produced a strong immunogenic response in horses. Antivenom raised against this venom mixture reacted with most venom components including crotamine and crotoxin, in contrast to the antivenom raised against crotamine-negative venom. Conclusions These results indicate that venomic databases and antivenomics analysis provide a useful approach for choosing the better venom mixture for antibody production and for the subsequent screening of antivenom cross-reactivity with relevant snake venom components. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Journal of Venomous Animals and Toxins including Tropical Diseases 23 1 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English |
topic |
Antivenom production Antivenomics Crotalus durissus Crotamine Crotoxin Geographic venom variation Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 |
spellingShingle |
Antivenom production Antivenomics Crotalus durissus Crotamine Crotoxin Geographic venom variation Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 Ricardo Teixeira-Araújo Patrícia Castanheira Leonora Brazil-Más Francisco Pontes Moema Leitão de Araújo Maria Lucia Machado Alves Russolina Benedeta Zingali Carlos Correa-Netto Antivenomics as a tool to improve the neutralizing capacity of the crotalic antivenom: a study with crotamine |
topic_facet |
Antivenom production Antivenomics Crotalus durissus Crotamine Crotoxin Geographic venom variation Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 |
description |
Abstract Background Snakebite treatment requires administration of an appropriate antivenom that should contain antibodies capable of neutralizing the venom. To achieve this goal, antivenom production must start from a suitable immunization protocol and proper venom mixtures. In Brazil, antivenom against South American rattlesnake (Crotalus durissus terrificus) bites is produced by public institutions based on the guidelines defined by the regulatory agency of the Brazilian Ministry of Health, ANVISA. However, each institution uses its own mixture of rattlesnake venom antigens. Previous works have shown that crotamine, a toxin found in Crolatus durissus venom, shows marked individual and populational variation. In addition, serum produced from crotamine-negative venoms fails to recognize this molecule. Methods In this work, we used an antivenomics approach to assess the cross-reactivity of crotalic antivenom manufactured by IVB towards crotamine-negative venom and a mixture of crotamine-negative/crotamine-positive venoms. Results We show that the venom mixture containing 20% crotamine and 57% crotoxin produced a strong immunogenic response in horses. Antivenom raised against this venom mixture reacted with most venom components including crotamine and crotoxin, in contrast to the antivenom raised against crotamine-negative venom. Conclusions These results indicate that venomic databases and antivenomics analysis provide a useful approach for choosing the better venom mixture for antibody production and for the subsequent screening of antivenom cross-reactivity with relevant snake venom components. |
format |
Article in Journal/Newspaper |
author |
Ricardo Teixeira-Araújo Patrícia Castanheira Leonora Brazil-Más Francisco Pontes Moema Leitão de Araújo Maria Lucia Machado Alves Russolina Benedeta Zingali Carlos Correa-Netto |
author_facet |
Ricardo Teixeira-Araújo Patrícia Castanheira Leonora Brazil-Más Francisco Pontes Moema Leitão de Araújo Maria Lucia Machado Alves Russolina Benedeta Zingali Carlos Correa-Netto |
author_sort |
Ricardo Teixeira-Araújo |
title |
Antivenomics as a tool to improve the neutralizing capacity of the crotalic antivenom: a study with crotamine |
title_short |
Antivenomics as a tool to improve the neutralizing capacity of the crotalic antivenom: a study with crotamine |
title_full |
Antivenomics as a tool to improve the neutralizing capacity of the crotalic antivenom: a study with crotamine |
title_fullStr |
Antivenomics as a tool to improve the neutralizing capacity of the crotalic antivenom: a study with crotamine |
title_full_unstemmed |
Antivenomics as a tool to improve the neutralizing capacity of the crotalic antivenom: a study with crotamine |
title_sort |
antivenomics as a tool to improve the neutralizing capacity of the crotalic antivenom: a study with crotamine |
publisher |
SciELO |
publishDate |
2017 |
url |
https://doi.org/10.1186/s40409-017-0118-7 https://doaj.org/article/3c21cf9986944e58888cfe311972b3a8 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Journal of Venomous Animals and Toxins including Tropical Diseases, Vol 23, Iss 1, Pp 1-8 (2017) |
op_relation |
http://link.springer.com/article/10.1186/s40409-017-0118-7 https://doaj.org/toc/1678-9199 doi:10.1186/s40409-017-0118-7 1678-9199 https://doaj.org/article/3c21cf9986944e58888cfe311972b3a8 |
op_doi |
https://doi.org/10.1186/s40409-017-0118-7 |
container_title |
Journal of Venomous Animals and Toxins including Tropical Diseases |
container_volume |
23 |
container_issue |
1 |
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1766342345250308096 |